Protocol for whole-mount preparation, clearing, and visualization of the adult zebrafish spinal cord structures
Summary: The vascular repair, a pivotal element of the spinal cord (SC) injury response, has been notably neglected in zebrafish research. Here, we detail the extraction and dissection of whole-mount zebrafish SCs and the optimized whole-mount immunofluorescence staining and clearing protocols for t...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724006567 |
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| author | Mariana Rebocho da Costa Ana Ribeiro Raquel Quitéria Dalila Neves-Silva Leonor Saúde |
| author_facet | Mariana Rebocho da Costa Ana Ribeiro Raquel Quitéria Dalila Neves-Silva Leonor Saúde |
| author_sort | Mariana Rebocho da Costa |
| collection | DOAJ |
| description | Summary: The vascular repair, a pivotal element of the spinal cord (SC) injury response, has been notably neglected in zebrafish research. Here, we detail the extraction and dissection of whole-mount zebrafish SCs and the optimized whole-mount immunofluorescence staining and clearing protocols for the visualization of SC structures, such as the vascular network. Additional downstream applications of these samples, from gelatin embedding to cryosectioning and tissue staining, can be done after clearing reversion and are also described in this work.For complete details on the use and execution of this protocol, please refer to Ribeiro et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-a3e75493502d410d887880713bd2ce8a |
| institution | OA Journals |
| issn | 2666-1667 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-a3e75493502d410d887880713bd2ce8a2025-08-20T02:39:03ZengElsevierSTAR Protocols2666-16672024-12-015410349110.1016/j.xpro.2024.103491Protocol for whole-mount preparation, clearing, and visualization of the adult zebrafish spinal cord structuresMariana Rebocho da Costa0Ana Ribeiro1Raquel Quitéria2Dalila Neves-Silva3Leonor Saúde4Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, Portugal; GIMM - Gulbenkian Institute for Molecular Medicina, Avenida Prof. Egas Moniz, 1649-035 Lisboa, Portugal; Corresponding authorInstituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, PortugalInstituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, PortugalInstituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, Portugal; GIMM - Gulbenkian Institute for Molecular Medicina, Avenida Prof. Egas Moniz, 1649-035 Lisboa, PortugalInstituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, Portugal; Instituto de Histologia e Biologia do Desenvolvimento, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, Portugal; GIMM - Gulbenkian Institute for Molecular Medicina, Avenida Prof. Egas Moniz, 1649-035 Lisboa, Portugal; Corresponding authorSummary: The vascular repair, a pivotal element of the spinal cord (SC) injury response, has been notably neglected in zebrafish research. Here, we detail the extraction and dissection of whole-mount zebrafish SCs and the optimized whole-mount immunofluorescence staining and clearing protocols for the visualization of SC structures, such as the vascular network. Additional downstream applications of these samples, from gelatin embedding to cryosectioning and tissue staining, can be done after clearing reversion and are also described in this work.For complete details on the use and execution of this protocol, please refer to Ribeiro et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724006567Cell BiologyMicroscopyModel OrganismsNeuroscience |
| spellingShingle | Mariana Rebocho da Costa Ana Ribeiro Raquel Quitéria Dalila Neves-Silva Leonor Saúde Protocol for whole-mount preparation, clearing, and visualization of the adult zebrafish spinal cord structures STAR Protocols Cell Biology Microscopy Model Organisms Neuroscience |
| title | Protocol for whole-mount preparation, clearing, and visualization of the adult zebrafish spinal cord structures |
| title_full | Protocol for whole-mount preparation, clearing, and visualization of the adult zebrafish spinal cord structures |
| title_fullStr | Protocol for whole-mount preparation, clearing, and visualization of the adult zebrafish spinal cord structures |
| title_full_unstemmed | Protocol for whole-mount preparation, clearing, and visualization of the adult zebrafish spinal cord structures |
| title_short | Protocol for whole-mount preparation, clearing, and visualization of the adult zebrafish spinal cord structures |
| title_sort | protocol for whole mount preparation clearing and visualization of the adult zebrafish spinal cord structures |
| topic | Cell Biology Microscopy Model Organisms Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724006567 |
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