In Vivo Assessment of Healing Potential of Ointments Containing Bee Products, Vegetal Extracts, and Polymers on Skin Lesions
<b>Background/Objectives:</b> The present experiment aimed to formulate four ointments that included mixtures of plant extracts (<i>Hippophae rhamnoides</i>, <i>Calendula officinalis</i>, <i>Arctium lappa</i>, and <i>Achillea millefolium</i>...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Pharmaceuticals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8247/18/1/65 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832587728480370688 |
|---|---|
| author | Calin Vasile Andritoiu Cristina Lungu Camelia Elena Iurciuc (Tincu) Corina Elena Andriescu Corneliu Havarneanu Marcel Popa Magdalena Cuciureanu Liliana Mititelu Tarţău Bianca Ivanescu |
| author_facet | Calin Vasile Andritoiu Cristina Lungu Camelia Elena Iurciuc (Tincu) Corina Elena Andriescu Corneliu Havarneanu Marcel Popa Magdalena Cuciureanu Liliana Mititelu Tarţău Bianca Ivanescu |
| author_sort | Calin Vasile Andritoiu |
| collection | DOAJ |
| description | <b>Background/Objectives:</b> The present experiment aimed to formulate four ointments that included mixtures of plant extracts (<i>Hippophae rhamnoides</i>, <i>Calendula officinalis</i>, <i>Arctium lappa</i>, and <i>Achillea millefolium</i>), apitherapy products (honey, propolis, and apilarnil) and natural polymers (collagen, chitosan, and the lyophilisate of egg white) in an ointment base. <b>Methods</b>: In order to investigate the therapeutic properties of the ointments, experimental in vivo injury models (linear incision, circular excision, and thermal burns) were performed on laboratory animals, namely Wistar rats. The treatment was applied topically, once a day, for 21 days. Clinical and macroscopic evaluation, determination of lesion shrinkage rate, re-epithelialization period, and histopathological examination were performed. <b>Results</b>: The results demonstrate that the tested ointments have a significant effect in healing skin lesions. On the ninth day of treatment, the wound contraction rate was 98.17 ± 0.15% for the mixed ointment group, compared to the negative control group’s rate of 14.85 ± 2.95%. At day 21, dermal collagenization and restoration of histological structure occurred for all treated groups. <b>Conclusions</b>: The tested ointments exerted in vivo wound healing and re-epithelialization effects on incision, excision, and thermal burn injuries. |
| format | Article |
| id | doaj-art-a3dd2a6e94334a9c8fdfddb565960c1e |
| institution | Kabale University |
| issn | 1424-8247 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj-art-a3dd2a6e94334a9c8fdfddb565960c1e2025-01-24T13:45:15ZengMDPI AGPharmaceuticals1424-82472025-01-011816510.3390/ph18010065In Vivo Assessment of Healing Potential of Ointments Containing Bee Products, Vegetal Extracts, and Polymers on Skin LesionsCalin Vasile Andritoiu0Cristina Lungu1Camelia Elena Iurciuc (Tincu)2Corina Elena Andriescu3Corneliu Havarneanu4Marcel Popa5Magdalena Cuciureanu6Liliana Mititelu Tarţău7Bianca Ivanescu8Apitherapy Medical Center, Balaneşti, 217036 Gorj, RomaniaDepartment of Pharmaceutical Botany, Faculty of Pharmacy, “Grigore T. Popa” University of Medicine and Pharmacy, 700115 Iaşi, RomaniaDepartment of Natural and Synthetic Polymers, Cristofor Simionescu Faculty of Chemical Engineering and Environmental Protection, Gheorghe Asachi Technical University of Iaşi, 700050 Iaşi, RomaniaDepartment of Pathology, Sf. Spiridon Emergency County Hospital, 700111 Iaşi, RomaniaFaculty of Psychology and Education Sciences, Alexandru Ioan Cuza University, 700554 Iaşi, RomaniaDepartment of Natural and Synthetic Polymers, Cristofor Simionescu Faculty of Chemical Engineering and Environmental Protection, Gheorghe Asachi Technical University of Iaşi, 700050 Iaşi, RomaniaDepartment of Pharmacology, Grigore T. Popa University of Medicine and Pharmacy, 700115 Iaşi, RomaniaDepartment of Pharmacology, Grigore T. Popa University of Medicine and Pharmacy, 700115 Iaşi, RomaniaDepartment of Pharmaceutical Botany, Faculty of Pharmacy, “Grigore T. Popa” University of Medicine and Pharmacy, 700115 Iaşi, Romania<b>Background/Objectives:</b> The present experiment aimed to formulate four ointments that included mixtures of plant extracts (<i>Hippophae rhamnoides</i>, <i>Calendula officinalis</i>, <i>Arctium lappa</i>, and <i>Achillea millefolium</i>), apitherapy products (honey, propolis, and apilarnil) and natural polymers (collagen, chitosan, and the lyophilisate of egg white) in an ointment base. <b>Methods</b>: In order to investigate the therapeutic properties of the ointments, experimental in vivo injury models (linear incision, circular excision, and thermal burns) were performed on laboratory animals, namely Wistar rats. The treatment was applied topically, once a day, for 21 days. Clinical and macroscopic evaluation, determination of lesion shrinkage rate, re-epithelialization period, and histopathological examination were performed. <b>Results</b>: The results demonstrate that the tested ointments have a significant effect in healing skin lesions. On the ninth day of treatment, the wound contraction rate was 98.17 ± 0.15% for the mixed ointment group, compared to the negative control group’s rate of 14.85 ± 2.95%. At day 21, dermal collagenization and restoration of histological structure occurred for all treated groups. <b>Conclusions</b>: The tested ointments exerted in vivo wound healing and re-epithelialization effects on incision, excision, and thermal burn injuries.https://www.mdpi.com/1424-8247/18/1/65wound healingapitherapy productsnatural polymersvegetal extracts |
| spellingShingle | Calin Vasile Andritoiu Cristina Lungu Camelia Elena Iurciuc (Tincu) Corina Elena Andriescu Corneliu Havarneanu Marcel Popa Magdalena Cuciureanu Liliana Mititelu Tarţău Bianca Ivanescu In Vivo Assessment of Healing Potential of Ointments Containing Bee Products, Vegetal Extracts, and Polymers on Skin Lesions Pharmaceuticals wound healing apitherapy products natural polymers vegetal extracts |
| title | In Vivo Assessment of Healing Potential of Ointments Containing Bee Products, Vegetal Extracts, and Polymers on Skin Lesions |
| title_full | In Vivo Assessment of Healing Potential of Ointments Containing Bee Products, Vegetal Extracts, and Polymers on Skin Lesions |
| title_fullStr | In Vivo Assessment of Healing Potential of Ointments Containing Bee Products, Vegetal Extracts, and Polymers on Skin Lesions |
| title_full_unstemmed | In Vivo Assessment of Healing Potential of Ointments Containing Bee Products, Vegetal Extracts, and Polymers on Skin Lesions |
| title_short | In Vivo Assessment of Healing Potential of Ointments Containing Bee Products, Vegetal Extracts, and Polymers on Skin Lesions |
| title_sort | in vivo assessment of healing potential of ointments containing bee products vegetal extracts and polymers on skin lesions |
| topic | wound healing apitherapy products natural polymers vegetal extracts |
| url | https://www.mdpi.com/1424-8247/18/1/65 |
| work_keys_str_mv | AT calinvasileandritoiu invivoassessmentofhealingpotentialofointmentscontainingbeeproductsvegetalextractsandpolymersonskinlesions AT cristinalungu invivoassessmentofhealingpotentialofointmentscontainingbeeproductsvegetalextractsandpolymersonskinlesions AT cameliaelenaiurciuctincu invivoassessmentofhealingpotentialofointmentscontainingbeeproductsvegetalextractsandpolymersonskinlesions AT corinaelenaandriescu invivoassessmentofhealingpotentialofointmentscontainingbeeproductsvegetalextractsandpolymersonskinlesions AT corneliuhavarneanu invivoassessmentofhealingpotentialofointmentscontainingbeeproductsvegetalextractsandpolymersonskinlesions AT marcelpopa invivoassessmentofhealingpotentialofointmentscontainingbeeproductsvegetalextractsandpolymersonskinlesions AT magdalenacuciureanu invivoassessmentofhealingpotentialofointmentscontainingbeeproductsvegetalextractsandpolymersonskinlesions AT lilianamititelutartau invivoassessmentofhealingpotentialofointmentscontainingbeeproductsvegetalextractsandpolymersonskinlesions AT biancaivanescu invivoassessmentofhealingpotentialofointmentscontainingbeeproductsvegetalextractsandpolymersonskinlesions |