In Vivo Assessment of Healing Potential of Ointments Containing Bee Products, Vegetal Extracts, and Polymers on Skin Lesions

<b>Background/Objectives:</b> The present experiment aimed to formulate four ointments that included mixtures of plant extracts (<i>Hippophae rhamnoides</i>, <i>Calendula officinalis</i>, <i>Arctium lappa</i>, and <i>Achillea millefolium</i>...

Full description

Saved in:
Bibliographic Details
Main Authors: Calin Vasile Andritoiu, Cristina Lungu, Camelia Elena Iurciuc (Tincu), Corina Elena Andriescu, Corneliu Havarneanu, Marcel Popa, Magdalena Cuciureanu, Liliana Mititelu Tarţău, Bianca Ivanescu
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Pharmaceuticals
Subjects:
Online Access:https://www.mdpi.com/1424-8247/18/1/65
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832587728480370688
author Calin Vasile Andritoiu
Cristina Lungu
Camelia Elena Iurciuc (Tincu)
Corina Elena Andriescu
Corneliu Havarneanu
Marcel Popa
Magdalena Cuciureanu
Liliana Mititelu Tarţău
Bianca Ivanescu
author_facet Calin Vasile Andritoiu
Cristina Lungu
Camelia Elena Iurciuc (Tincu)
Corina Elena Andriescu
Corneliu Havarneanu
Marcel Popa
Magdalena Cuciureanu
Liliana Mititelu Tarţău
Bianca Ivanescu
author_sort Calin Vasile Andritoiu
collection DOAJ
description <b>Background/Objectives:</b> The present experiment aimed to formulate four ointments that included mixtures of plant extracts (<i>Hippophae rhamnoides</i>, <i>Calendula officinalis</i>, <i>Arctium lappa</i>, and <i>Achillea millefolium</i>), apitherapy products (honey, propolis, and apilarnil) and natural polymers (collagen, chitosan, and the lyophilisate of egg white) in an ointment base. <b>Methods</b>: In order to investigate the therapeutic properties of the ointments, experimental in vivo injury models (linear incision, circular excision, and thermal burns) were performed on laboratory animals, namely Wistar rats. The treatment was applied topically, once a day, for 21 days. Clinical and macroscopic evaluation, determination of lesion shrinkage rate, re-epithelialization period, and histopathological examination were performed. <b>Results</b>: The results demonstrate that the tested ointments have a significant effect in healing skin lesions. On the ninth day of treatment, the wound contraction rate was 98.17 ± 0.15% for the mixed ointment group, compared to the negative control group’s rate of 14.85 ± 2.95%. At day 21, dermal collagenization and restoration of histological structure occurred for all treated groups. <b>Conclusions</b>: The tested ointments exerted in vivo wound healing and re-epithelialization effects on incision, excision, and thermal burn injuries.
format Article
id doaj-art-a3dd2a6e94334a9c8fdfddb565960c1e
institution Kabale University
issn 1424-8247
language English
publishDate 2025-01-01
publisher MDPI AG
record_format Article
series Pharmaceuticals
spelling doaj-art-a3dd2a6e94334a9c8fdfddb565960c1e2025-01-24T13:45:15ZengMDPI AGPharmaceuticals1424-82472025-01-011816510.3390/ph18010065In Vivo Assessment of Healing Potential of Ointments Containing Bee Products, Vegetal Extracts, and Polymers on Skin LesionsCalin Vasile Andritoiu0Cristina Lungu1Camelia Elena Iurciuc (Tincu)2Corina Elena Andriescu3Corneliu Havarneanu4Marcel Popa5Magdalena Cuciureanu6Liliana Mititelu Tarţău7Bianca Ivanescu8Apitherapy Medical Center, Balaneşti, 217036 Gorj, RomaniaDepartment of Pharmaceutical Botany, Faculty of Pharmacy, “Grigore T. Popa” University of Medicine and Pharmacy, 700115 Iaşi, RomaniaDepartment of Natural and Synthetic Polymers, Cristofor Simionescu Faculty of Chemical Engineering and Environmental Protection, Gheorghe Asachi Technical University of Iaşi, 700050 Iaşi, RomaniaDepartment of Pathology, Sf. Spiridon Emergency County Hospital, 700111 Iaşi, RomaniaFaculty of Psychology and Education Sciences, Alexandru Ioan Cuza University, 700554 Iaşi, RomaniaDepartment of Natural and Synthetic Polymers, Cristofor Simionescu Faculty of Chemical Engineering and Environmental Protection, Gheorghe Asachi Technical University of Iaşi, 700050 Iaşi, RomaniaDepartment of Pharmacology, Grigore T. Popa University of Medicine and Pharmacy, 700115 Iaşi, RomaniaDepartment of Pharmacology, Grigore T. Popa University of Medicine and Pharmacy, 700115 Iaşi, RomaniaDepartment of Pharmaceutical Botany, Faculty of Pharmacy, “Grigore T. Popa” University of Medicine and Pharmacy, 700115 Iaşi, Romania<b>Background/Objectives:</b> The present experiment aimed to formulate four ointments that included mixtures of plant extracts (<i>Hippophae rhamnoides</i>, <i>Calendula officinalis</i>, <i>Arctium lappa</i>, and <i>Achillea millefolium</i>), apitherapy products (honey, propolis, and apilarnil) and natural polymers (collagen, chitosan, and the lyophilisate of egg white) in an ointment base. <b>Methods</b>: In order to investigate the therapeutic properties of the ointments, experimental in vivo injury models (linear incision, circular excision, and thermal burns) were performed on laboratory animals, namely Wistar rats. The treatment was applied topically, once a day, for 21 days. Clinical and macroscopic evaluation, determination of lesion shrinkage rate, re-epithelialization period, and histopathological examination were performed. <b>Results</b>: The results demonstrate that the tested ointments have a significant effect in healing skin lesions. On the ninth day of treatment, the wound contraction rate was 98.17 ± 0.15% for the mixed ointment group, compared to the negative control group’s rate of 14.85 ± 2.95%. At day 21, dermal collagenization and restoration of histological structure occurred for all treated groups. <b>Conclusions</b>: The tested ointments exerted in vivo wound healing and re-epithelialization effects on incision, excision, and thermal burn injuries.https://www.mdpi.com/1424-8247/18/1/65wound healingapitherapy productsnatural polymersvegetal extracts
spellingShingle Calin Vasile Andritoiu
Cristina Lungu
Camelia Elena Iurciuc (Tincu)
Corina Elena Andriescu
Corneliu Havarneanu
Marcel Popa
Magdalena Cuciureanu
Liliana Mititelu Tarţău
Bianca Ivanescu
In Vivo Assessment of Healing Potential of Ointments Containing Bee Products, Vegetal Extracts, and Polymers on Skin Lesions
Pharmaceuticals
wound healing
apitherapy products
natural polymers
vegetal extracts
title In Vivo Assessment of Healing Potential of Ointments Containing Bee Products, Vegetal Extracts, and Polymers on Skin Lesions
title_full In Vivo Assessment of Healing Potential of Ointments Containing Bee Products, Vegetal Extracts, and Polymers on Skin Lesions
title_fullStr In Vivo Assessment of Healing Potential of Ointments Containing Bee Products, Vegetal Extracts, and Polymers on Skin Lesions
title_full_unstemmed In Vivo Assessment of Healing Potential of Ointments Containing Bee Products, Vegetal Extracts, and Polymers on Skin Lesions
title_short In Vivo Assessment of Healing Potential of Ointments Containing Bee Products, Vegetal Extracts, and Polymers on Skin Lesions
title_sort in vivo assessment of healing potential of ointments containing bee products vegetal extracts and polymers on skin lesions
topic wound healing
apitherapy products
natural polymers
vegetal extracts
url https://www.mdpi.com/1424-8247/18/1/65
work_keys_str_mv AT calinvasileandritoiu invivoassessmentofhealingpotentialofointmentscontainingbeeproductsvegetalextractsandpolymersonskinlesions
AT cristinalungu invivoassessmentofhealingpotentialofointmentscontainingbeeproductsvegetalextractsandpolymersonskinlesions
AT cameliaelenaiurciuctincu invivoassessmentofhealingpotentialofointmentscontainingbeeproductsvegetalextractsandpolymersonskinlesions
AT corinaelenaandriescu invivoassessmentofhealingpotentialofointmentscontainingbeeproductsvegetalextractsandpolymersonskinlesions
AT corneliuhavarneanu invivoassessmentofhealingpotentialofointmentscontainingbeeproductsvegetalextractsandpolymersonskinlesions
AT marcelpopa invivoassessmentofhealingpotentialofointmentscontainingbeeproductsvegetalextractsandpolymersonskinlesions
AT magdalenacuciureanu invivoassessmentofhealingpotentialofointmentscontainingbeeproductsvegetalextractsandpolymersonskinlesions
AT lilianamititelutartau invivoassessmentofhealingpotentialofointmentscontainingbeeproductsvegetalextractsandpolymersonskinlesions
AT biancaivanescu invivoassessmentofhealingpotentialofointmentscontainingbeeproductsvegetalextractsandpolymersonskinlesions