Antibacterial effects of nano-emulsified cumin oil on performance and carcass characteristics in weaning rabbits infected by Clostridium perfringens type A
Abstract Background This study aimed to evaluate the antibacterial effects and growth-promoting potential of nano-emulsified cumin oil (NECO) in vitro and in vivo trials using newly weaned Gabaly rabbits. NECO was tested as both a preventive and curative agent against Clostridium perfringens type A...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2025-07-01
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| Series: | Beni-Suef University Journal of Basic and Applied Sciences |
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| Online Access: | https://doi.org/10.1186/s43088-025-00659-1 |
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| author | Reda. R. Fathy Mohamed Abaza Zangabel Mohamed Aya. H. Tantawy Mona Abdallah Noura M. khalaf Sherein Mohamed |
| author_facet | Reda. R. Fathy Mohamed Abaza Zangabel Mohamed Aya. H. Tantawy Mona Abdallah Noura M. khalaf Sherein Mohamed |
| author_sort | Reda. R. Fathy |
| collection | DOAJ |
| description | Abstract Background This study aimed to evaluate the antibacterial effects and growth-promoting potential of nano-emulsified cumin oil (NECO) in vitro and in vivo trials using newly weaned Gabaly rabbits. NECO was tested as both a preventive and curative agent against Clostridium perfringens type A infection through dietary supplementation. Methods The study included two experimental phases: (1) an in vitro trial to determine the bacterial inhibition potential of different NECO concentrations (0.1, 0.2, 0.5, and 1 mL) and (2) an in vivo trial using 120 four-week-old newly weaned male Gabaly rabbits (0.544 kg average body weight) allocated into five groups (n = 24/group were divided into 3 replicates, 8 animals each). The groups were: G1 (negative control, no infection or supplement), G2 (NECO control, supplemented with 10 mL/kg diet), G3 (positive control, infected with C. perfringens type A, no supplement), G4 (preventive NECO, supplemented with 10 mL/kg diet before infection), and G5 (curative NECO, supplemented with 10 mL/kg diet after infection). NECO was formulated as a stable nano-emulsion using a high-shear homogenizer. Results The in vitro study demonstrated that NECO inhibited C. perfringens growth at all tested concentrations, with complete inhibition observed at 1 mL. Based on this, the 1 mL concentration was selected for in vivo trials. Infected rabbits (G3) exhibited clinical signs including anorexia, depression, severe diarrhea, and bloat, with a morbidity rate of 90% and a 35% mortality rate by the 5th day post-challenge. NECO supplementation significantly improved (p < 0.05) final body weight (FBW), total weight gain, and average daily gain (ADG) in G2 compared to G3. While G4 and G5 did not significantly differ in FBW or ADG from the control (G1), all NECO-supplemented groups had improved feed conversion ratio (FCR) compared to G3 (p < 0.05). The best FCR was observed in G2 (3.80), followed by G4 (4.51) and G5 (4.77), while the worst was in G3 (5.31). Histopathological and postmortem findings confirmed reduced disease severity with NECO supplementation. Conclusion These results suggest that NECO serves as a natural antibacterial alternative, effectively mitigating C. perfringens infection while enhancing growth performance. |
| format | Article |
| id | doaj-art-a3d5dbcb769c482db340cfcf5d4df4cb |
| institution | Kabale University |
| issn | 2314-8543 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Beni-Suef University Journal of Basic and Applied Sciences |
| spelling | doaj-art-a3d5dbcb769c482db340cfcf5d4df4cb2025-08-20T03:46:19ZengSpringerOpenBeni-Suef University Journal of Basic and Applied Sciences2314-85432025-07-0114111710.1186/s43088-025-00659-1Antibacterial effects of nano-emulsified cumin oil on performance and carcass characteristics in weaning rabbits infected by Clostridium perfringens type AReda. R. Fathy0Mohamed Abaza1Zangabel Mohamed2Aya. H. Tantawy3Mona Abdallah4Noura M. khalaf5Sherein Mohamed6Benha UniversityBenha UniversityBenha UniversityBenha UniversityBenha UniversityE Veterinary Serum and Vaccine Research InstituteBenha UniversityAbstract Background This study aimed to evaluate the antibacterial effects and growth-promoting potential of nano-emulsified cumin oil (NECO) in vitro and in vivo trials using newly weaned Gabaly rabbits. NECO was tested as both a preventive and curative agent against Clostridium perfringens type A infection through dietary supplementation. Methods The study included two experimental phases: (1) an in vitro trial to determine the bacterial inhibition potential of different NECO concentrations (0.1, 0.2, 0.5, and 1 mL) and (2) an in vivo trial using 120 four-week-old newly weaned male Gabaly rabbits (0.544 kg average body weight) allocated into five groups (n = 24/group were divided into 3 replicates, 8 animals each). The groups were: G1 (negative control, no infection or supplement), G2 (NECO control, supplemented with 10 mL/kg diet), G3 (positive control, infected with C. perfringens type A, no supplement), G4 (preventive NECO, supplemented with 10 mL/kg diet before infection), and G5 (curative NECO, supplemented with 10 mL/kg diet after infection). NECO was formulated as a stable nano-emulsion using a high-shear homogenizer. Results The in vitro study demonstrated that NECO inhibited C. perfringens growth at all tested concentrations, with complete inhibition observed at 1 mL. Based on this, the 1 mL concentration was selected for in vivo trials. Infected rabbits (G3) exhibited clinical signs including anorexia, depression, severe diarrhea, and bloat, with a morbidity rate of 90% and a 35% mortality rate by the 5th day post-challenge. NECO supplementation significantly improved (p < 0.05) final body weight (FBW), total weight gain, and average daily gain (ADG) in G2 compared to G3. While G4 and G5 did not significantly differ in FBW or ADG from the control (G1), all NECO-supplemented groups had improved feed conversion ratio (FCR) compared to G3 (p < 0.05). The best FCR was observed in G2 (3.80), followed by G4 (4.51) and G5 (4.77), while the worst was in G3 (5.31). Histopathological and postmortem findings confirmed reduced disease severity with NECO supplementation. Conclusion These results suggest that NECO serves as a natural antibacterial alternative, effectively mitigating C. perfringens infection while enhancing growth performance.https://doi.org/10.1186/s43088-025-00659-1CuminEssential oilNano-emulsionPerformanceRabbitClostridium |
| spellingShingle | Reda. R. Fathy Mohamed Abaza Zangabel Mohamed Aya. H. Tantawy Mona Abdallah Noura M. khalaf Sherein Mohamed Antibacterial effects of nano-emulsified cumin oil on performance and carcass characteristics in weaning rabbits infected by Clostridium perfringens type A Beni-Suef University Journal of Basic and Applied Sciences Cumin Essential oil Nano-emulsion Performance Rabbit Clostridium |
| title | Antibacterial effects of nano-emulsified cumin oil on performance and carcass characteristics in weaning rabbits infected by Clostridium perfringens type A |
| title_full | Antibacterial effects of nano-emulsified cumin oil on performance and carcass characteristics in weaning rabbits infected by Clostridium perfringens type A |
| title_fullStr | Antibacterial effects of nano-emulsified cumin oil on performance and carcass characteristics in weaning rabbits infected by Clostridium perfringens type A |
| title_full_unstemmed | Antibacterial effects of nano-emulsified cumin oil on performance and carcass characteristics in weaning rabbits infected by Clostridium perfringens type A |
| title_short | Antibacterial effects of nano-emulsified cumin oil on performance and carcass characteristics in weaning rabbits infected by Clostridium perfringens type A |
| title_sort | antibacterial effects of nano emulsified cumin oil on performance and carcass characteristics in weaning rabbits infected by clostridium perfringens type a |
| topic | Cumin Essential oil Nano-emulsion Performance Rabbit Clostridium |
| url | https://doi.org/10.1186/s43088-025-00659-1 |
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