POLE2 silencing inhibits the progression of colorectal carcinoma cells via wnt signaling axis
Colorectal cancer (CRC) is one of the most common malignant carcinoma worldwide. DNA polymerase epsilon 2, accessory subunit (POLE2) participates in DNA replication, repair, and cell cycle control, but its association with CRC development remains unclear. In the present study, the differentially exp...
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Taylor & Francis Group
2024-12-01
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| Series: | Cancer Biology & Therapy |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/15384047.2024.2392339 |
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| author | Weihua Jian Lei Zhang |
| author_facet | Weihua Jian Lei Zhang |
| author_sort | Weihua Jian |
| collection | DOAJ |
| description | Colorectal cancer (CRC) is one of the most common malignant carcinoma worldwide. DNA polymerase epsilon 2, accessory subunit (POLE2) participates in DNA replication, repair, and cell cycle control, but its association with CRC development remains unclear. In the present study, the differentially expressed genes (DEGs) in CRC were screened from bioinformatics analysis based on GEO database. RT-qPCR was used to assess mRNA expression. CCK-8 and colony formation assays were applied for the evaluation of cell proliferation. Wound healing and transwell assays were used to detect cell migration and invasion. Protein levels were determined by Western blotting assay. We found that POLE2 was highly expressed in CRC tissues and cell lines. Inhibition of POLE2 suppressed the proliferation, migration and invasion of CRC cells. Mechanistically, Wnt/β-catenin signaling pathway was inactivated by inhibition of POLE2. Activation of Wnt/β-catenin pathway can reverse the function of POLE2 knockdown on CRC cells. In vivo studies demonstrated that POLE2 silencing could notably inhibit the growth of tumors, which was consistent with the results in vitro. In conclusion, we found POLE2 as a novel oncogene in CRC, providing a potential therapeutic or diagnostic target in CRC. |
| format | Article |
| id | doaj-art-a3d4be6af85e48d68052fd7f9448c2cf |
| institution | DOAJ |
| issn | 1538-4047 1555-8576 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
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| series | Cancer Biology & Therapy |
| spelling | doaj-art-a3d4be6af85e48d68052fd7f9448c2cf2025-08-20T03:22:00ZengTaylor & Francis GroupCancer Biology & Therapy1538-40471555-85762024-12-0125110.1080/15384047.2024.2392339POLE2 silencing inhibits the progression of colorectal carcinoma cells via wnt signaling axisWeihua Jian0Lei Zhang1Department of General Surgery, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, ChinaDepartment of Second General Surgery, Guangdong Second Provincial General Hospital, Guangzhou, ChinaColorectal cancer (CRC) is one of the most common malignant carcinoma worldwide. DNA polymerase epsilon 2, accessory subunit (POLE2) participates in DNA replication, repair, and cell cycle control, but its association with CRC development remains unclear. In the present study, the differentially expressed genes (DEGs) in CRC were screened from bioinformatics analysis based on GEO database. RT-qPCR was used to assess mRNA expression. CCK-8 and colony formation assays were applied for the evaluation of cell proliferation. Wound healing and transwell assays were used to detect cell migration and invasion. Protein levels were determined by Western blotting assay. We found that POLE2 was highly expressed in CRC tissues and cell lines. Inhibition of POLE2 suppressed the proliferation, migration and invasion of CRC cells. Mechanistically, Wnt/β-catenin signaling pathway was inactivated by inhibition of POLE2. Activation of Wnt/β-catenin pathway can reverse the function of POLE2 knockdown on CRC cells. In vivo studies demonstrated that POLE2 silencing could notably inhibit the growth of tumors, which was consistent with the results in vitro. In conclusion, we found POLE2 as a novel oncogene in CRC, providing a potential therapeutic or diagnostic target in CRC.https://www.tandfonline.com/doi/10.1080/15384047.2024.2392339Colorectal cancerPOLE2Wnt/β-cateninproliferationinvasion |
| spellingShingle | Weihua Jian Lei Zhang POLE2 silencing inhibits the progression of colorectal carcinoma cells via wnt signaling axis Cancer Biology & Therapy Colorectal cancer POLE2 Wnt/β-catenin proliferation invasion |
| title | POLE2 silencing inhibits the progression of colorectal carcinoma cells via wnt signaling axis |
| title_full | POLE2 silencing inhibits the progression of colorectal carcinoma cells via wnt signaling axis |
| title_fullStr | POLE2 silencing inhibits the progression of colorectal carcinoma cells via wnt signaling axis |
| title_full_unstemmed | POLE2 silencing inhibits the progression of colorectal carcinoma cells via wnt signaling axis |
| title_short | POLE2 silencing inhibits the progression of colorectal carcinoma cells via wnt signaling axis |
| title_sort | pole2 silencing inhibits the progression of colorectal carcinoma cells via wnt signaling axis |
| topic | Colorectal cancer POLE2 Wnt/β-catenin proliferation invasion |
| url | https://www.tandfonline.com/doi/10.1080/15384047.2024.2392339 |
| work_keys_str_mv | AT weihuajian pole2silencinginhibitstheprogressionofcolorectalcarcinomacellsviawntsignalingaxis AT leizhang pole2silencinginhibitstheprogressionofcolorectalcarcinomacellsviawntsignalingaxis |