Direct transdifferentiation of tumorigenic melanoma cells induces tumor cell reversion

Abstract Melanoma is an aggressive skin cancer and highly lethal at advanced stages due to its high tumorigenicity and metastatic capacity. Changing the phenotype of cancer cells from one lineage to another, a process called transdifferentiation, leads to tumor cell reversion, which goes along with...

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Main Authors: Yiman Wang, Ke Liu, Yuxin Zhang, Daniel Novak, Aniello Federico, Cai Xu, Sandra Horschitz, Marlene Vierthaler, Qian Sun, Nina Wang, Juliane Poelchen, Tamara Steinfass, Laura Hüser, Moritz Mall, Viktor Umansky, Jochen Utikal
Format: Article
Language:English
Published: Nature Publishing Group 2025-07-01
Series:Cell Death and Disease
Online Access:https://doi.org/10.1038/s41419-025-07863-y
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author Yiman Wang
Ke Liu
Yuxin Zhang
Daniel Novak
Aniello Federico
Cai Xu
Sandra Horschitz
Marlene Vierthaler
Qian Sun
Nina Wang
Juliane Poelchen
Tamara Steinfass
Laura Hüser
Moritz Mall
Viktor Umansky
Jochen Utikal
author_facet Yiman Wang
Ke Liu
Yuxin Zhang
Daniel Novak
Aniello Federico
Cai Xu
Sandra Horschitz
Marlene Vierthaler
Qian Sun
Nina Wang
Juliane Poelchen
Tamara Steinfass
Laura Hüser
Moritz Mall
Viktor Umansky
Jochen Utikal
author_sort Yiman Wang
collection DOAJ
description Abstract Melanoma is an aggressive skin cancer and highly lethal at advanced stages due to its high tumorigenicity and metastatic capacity. Changing the phenotype of cancer cells from one lineage to another, a process called transdifferentiation, leads to tumor cell reversion, which goes along with a drastic reduction of their tumorigenicity. Via ectopic overexpression of four neuronal transcription factors, we transdifferentiated melanoma cells into neuron-like cells expressing neuronal markers and showing a neuron-like morphology. Moreover, the tumorigenic and metastatic potential of transdifferentiated cells in vitro and in vivo was significantly reduced. Transdifferentiated cells were also more sensitive to radiotherapy compared with their parental counterparts. We conclude that transdifferentiation of cancer cells into terminally differentiated neuron-like cells might represent a prospective new therapeutic approach for the treatment of melanoma.
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series Cell Death and Disease
spelling doaj-art-a3d49f87252e41d294154ea7983ee81d2025-08-20T03:06:09ZengNature Publishing GroupCell Death and Disease2041-48892025-07-0116111210.1038/s41419-025-07863-yDirect transdifferentiation of tumorigenic melanoma cells induces tumor cell reversionYiman Wang0Ke Liu1Yuxin Zhang2Daniel Novak3Aniello Federico4Cai Xu5Sandra Horschitz6Marlene Vierthaler7Qian Sun8Nina Wang9Juliane Poelchen10Tamara Steinfass11Laura Hüser12Moritz Mall13Viktor Umansky14Jochen Utikal15Skin Cancer Unit, German Cancer Research Center (DKFZ)Skin Cancer Unit, German Cancer Research Center (DKFZ)Skin Cancer Unit, German Cancer Research Center (DKFZ)Skin Cancer Unit, German Cancer Research Center (DKFZ)Skin Cancer Unit, German Cancer Research Center (DKFZ)Radiooncology/Radiobiology Department, German Cancer Research Center (DKFZ)Department of Translational Brain Research, Central Institute of Mental Health (ZI), University of Heidelberg/Medical FacultySkin Cancer Unit, German Cancer Research Center (DKFZ)Skin Cancer Unit, German Cancer Research Center (DKFZ)Skin Cancer Unit, German Cancer Research Center (DKFZ)Skin Cancer Unit, German Cancer Research Center (DKFZ)Skin Cancer Unit, German Cancer Research Center (DKFZ)Skin Cancer Unit, German Cancer Research Center (DKFZ)Cell Fate Engineering and Disease Modeling Group, German Cancer Research Center (DKFZ) and DKFZ-ZMBH AllianceSkin Cancer Unit, German Cancer Research Center (DKFZ)Skin Cancer Unit, German Cancer Research Center (DKFZ)Abstract Melanoma is an aggressive skin cancer and highly lethal at advanced stages due to its high tumorigenicity and metastatic capacity. Changing the phenotype of cancer cells from one lineage to another, a process called transdifferentiation, leads to tumor cell reversion, which goes along with a drastic reduction of their tumorigenicity. Via ectopic overexpression of four neuronal transcription factors, we transdifferentiated melanoma cells into neuron-like cells expressing neuronal markers and showing a neuron-like morphology. Moreover, the tumorigenic and metastatic potential of transdifferentiated cells in vitro and in vivo was significantly reduced. Transdifferentiated cells were also more sensitive to radiotherapy compared with their parental counterparts. We conclude that transdifferentiation of cancer cells into terminally differentiated neuron-like cells might represent a prospective new therapeutic approach for the treatment of melanoma.https://doi.org/10.1038/s41419-025-07863-y
spellingShingle Yiman Wang
Ke Liu
Yuxin Zhang
Daniel Novak
Aniello Federico
Cai Xu
Sandra Horschitz
Marlene Vierthaler
Qian Sun
Nina Wang
Juliane Poelchen
Tamara Steinfass
Laura Hüser
Moritz Mall
Viktor Umansky
Jochen Utikal
Direct transdifferentiation of tumorigenic melanoma cells induces tumor cell reversion
Cell Death and Disease
title Direct transdifferentiation of tumorigenic melanoma cells induces tumor cell reversion
title_full Direct transdifferentiation of tumorigenic melanoma cells induces tumor cell reversion
title_fullStr Direct transdifferentiation of tumorigenic melanoma cells induces tumor cell reversion
title_full_unstemmed Direct transdifferentiation of tumorigenic melanoma cells induces tumor cell reversion
title_short Direct transdifferentiation of tumorigenic melanoma cells induces tumor cell reversion
title_sort direct transdifferentiation of tumorigenic melanoma cells induces tumor cell reversion
url https://doi.org/10.1038/s41419-025-07863-y
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