Novel Orthohantavirus Associated with Hantavirus Pulmonary Syndrome in Northern Argentina
In this work, we performed the genetic characterization of a new variant of orthohantavirus associated with a fatal case of hantavirus pulmonary syndrome, outside the known endemic region, in northwestern Argentina. We first confirmed an orthohantavirus infection by ELISA, testing for the detection...
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2025-05-01
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| Online Access: | https://www.mdpi.com/1999-4915/17/5/717 |
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| author | Carla M. Bellomo Sebastian Kehl Daniel Oscar Alonso Walter López Flavia Cassinelli Rocío María Coelho Gabriela Bravo Sara Aguirre Marcela Dib Natalia Periolo Concepción Toscano José Gil Francisco García Campos Ignacio Ferro Valeria Paula Martinez |
| author_facet | Carla M. Bellomo Sebastian Kehl Daniel Oscar Alonso Walter López Flavia Cassinelli Rocío María Coelho Gabriela Bravo Sara Aguirre Marcela Dib Natalia Periolo Concepción Toscano José Gil Francisco García Campos Ignacio Ferro Valeria Paula Martinez |
| author_sort | Carla M. Bellomo |
| collection | DOAJ |
| description | In this work, we performed the genetic characterization of a new variant of orthohantavirus associated with a fatal case of hantavirus pulmonary syndrome, outside the known endemic region, in northwestern Argentina. We first confirmed an orthohantavirus infection by ELISA, testing for the detection of IgM and IgG antibodies. Then, we extracted RNA from 100 microliters of serum, the only sample available, followed by RT-PCR. The amplicons were sequenced using Sanger and next-generation sequencing technology. We obtained partial sequences of 1253 bp, 799 bp and 1675 bp from the S-, M- and L-segments, respectively, showing low sequence identities with all the previously characterized hantaviruses (10.9%, 13.5% and 15.1% of the divergence, respectively). The phylogenetic analysis showed that this virus belongs to the <i>Orthohantavirus andesense</i> species (ANDV), and among the ANDV-like variants, it is more closely related to the Lechiguanas clade. Similar percentages of divergence were considered sufficient to distinguish AND-like variants in previous works. As the patient had no travel history before the onset of disease was reported, we conducted rodent surveys to confirm the presence of reservoirs. The rodent assemblage was compatible with the transitional zone among different ecoregions (Yungas, Chaco and Monte). Moreover, one of the species captured, <i>Oligoryzomys flavescens</i>, was previously described as a reservoir of hantavirus. This species may either host several variants across its range or encompass a species complex, as proposed by some authors. |
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| institution | OA Journals |
| issn | 1999-4915 |
| language | English |
| publishDate | 2025-05-01 |
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| record_format | Article |
| series | Viruses |
| spelling | doaj-art-a3d290ab30fe48569bcb71c5c0b2876a2025-08-20T01:56:45ZengMDPI AGViruses1999-49152025-05-0117571710.3390/v17050717Novel Orthohantavirus Associated with Hantavirus Pulmonary Syndrome in Northern ArgentinaCarla M. Bellomo0Sebastian Kehl1Daniel Oscar Alonso2Walter López3Flavia Cassinelli4Rocío María Coelho5Gabriela Bravo6Sara Aguirre7Marcela Dib8Natalia Periolo9Concepción Toscano10José Gil11Francisco García Campos12Ignacio Ferro13Valeria Paula Martinez14Instituto Nacional de Enfermedades Infecciosas (INEI)—Administración Nacional de Laboratorios e Institutos de Salud (ANLIS) Malbran, Buenos Aires C1282, ArgentinaInstituto Nacional de Enfermedades Infecciosas (INEI)—Administración Nacional de Laboratorios e Institutos de Salud (ANLIS) Malbran, Buenos Aires C1282, ArgentinaInstituto Nacional de Enfermedades Infecciosas (INEI)—Administración Nacional de Laboratorios e Institutos de Salud (ANLIS) Malbran, Buenos Aires C1282, ArgentinaInstituto de Investigaciones de Enfermedades Tropicales-IIET, Universidad Nacional de Salta (UNSa), Salta A4400, ArgentinaInstituto de Ecorregiones Andinas (INECOA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Jujuy (UNJu), San Salvador de Jujuy Y4600, ArgentinaInstituto Nacional de Enfermedades Infecciosas (INEI)—Administración Nacional de Laboratorios e Institutos de Salud (ANLIS) Malbran, Buenos Aires C1282, ArgentinaHospital Señor Del Milagro, Salta A4400, ArgentinaInstituto de Investigaciones en Energía No Convencional (INENCO), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Salta (UNSa), Salta A4400, ArgentinaHospital Señor Del Milagro, Salta A4400, ArgentinaInstituto Nacional de Enfermedades Infecciosas (INEI)—Administración Nacional de Laboratorios e Institutos de Salud (ANLIS) Malbran, Buenos Aires C1282, ArgentinaHospital Señor Del Milagro, Salta A4400, ArgentinaInstituto de Investigaciones en Energía No Convencional (INENCO), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Salta (UNSa), Salta A4400, ArgentinaDirección General de Coordinación Epidemiológica, Ministerio de Salud Pública de Salta, Salta A4400, ArgentinaInstituto de Ecorregiones Andinas (INECOA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Jujuy (UNJu), San Salvador de Jujuy Y4600, ArgentinaInstituto Nacional de Enfermedades Infecciosas (INEI)—Administración Nacional de Laboratorios e Institutos de Salud (ANLIS) Malbran, Buenos Aires C1282, ArgentinaIn this work, we performed the genetic characterization of a new variant of orthohantavirus associated with a fatal case of hantavirus pulmonary syndrome, outside the known endemic region, in northwestern Argentina. We first confirmed an orthohantavirus infection by ELISA, testing for the detection of IgM and IgG antibodies. Then, we extracted RNA from 100 microliters of serum, the only sample available, followed by RT-PCR. The amplicons were sequenced using Sanger and next-generation sequencing technology. We obtained partial sequences of 1253 bp, 799 bp and 1675 bp from the S-, M- and L-segments, respectively, showing low sequence identities with all the previously characterized hantaviruses (10.9%, 13.5% and 15.1% of the divergence, respectively). The phylogenetic analysis showed that this virus belongs to the <i>Orthohantavirus andesense</i> species (ANDV), and among the ANDV-like variants, it is more closely related to the Lechiguanas clade. Similar percentages of divergence were considered sufficient to distinguish AND-like variants in previous works. As the patient had no travel history before the onset of disease was reported, we conducted rodent surveys to confirm the presence of reservoirs. The rodent assemblage was compatible with the transitional zone among different ecoregions (Yungas, Chaco and Monte). Moreover, one of the species captured, <i>Oligoryzomys flavescens</i>, was previously described as a reservoir of hantavirus. This species may either host several variants across its range or encompass a species complex, as proposed by some authors.https://www.mdpi.com/1999-4915/17/5/717orthohantavirushantavirus pulmonary syndromeSaltaArgentinazoonosisinfectious diseases |
| spellingShingle | Carla M. Bellomo Sebastian Kehl Daniel Oscar Alonso Walter López Flavia Cassinelli Rocío María Coelho Gabriela Bravo Sara Aguirre Marcela Dib Natalia Periolo Concepción Toscano José Gil Francisco García Campos Ignacio Ferro Valeria Paula Martinez Novel Orthohantavirus Associated with Hantavirus Pulmonary Syndrome in Northern Argentina Viruses orthohantavirus hantavirus pulmonary syndrome Salta Argentina zoonosis infectious diseases |
| title | Novel Orthohantavirus Associated with Hantavirus Pulmonary Syndrome in Northern Argentina |
| title_full | Novel Orthohantavirus Associated with Hantavirus Pulmonary Syndrome in Northern Argentina |
| title_fullStr | Novel Orthohantavirus Associated with Hantavirus Pulmonary Syndrome in Northern Argentina |
| title_full_unstemmed | Novel Orthohantavirus Associated with Hantavirus Pulmonary Syndrome in Northern Argentina |
| title_short | Novel Orthohantavirus Associated with Hantavirus Pulmonary Syndrome in Northern Argentina |
| title_sort | novel orthohantavirus associated with hantavirus pulmonary syndrome in northern argentina |
| topic | orthohantavirus hantavirus pulmonary syndrome Salta Argentina zoonosis infectious diseases |
| url | https://www.mdpi.com/1999-4915/17/5/717 |
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