Effects of different Lys/Met ratios on the antioxidant capacity, tissue morphology, and fatty acid composition of subcutaneous fat in Tibetan sheep on low-protein diets: a lipidomic analysis

IntroductionThis study employed lipidomics to investigate the effects of varying lysine (Lys)- to-methionine (Met) ratios on the antioxidant capacity, tissue morphology, and fatty acid composition of subcutaneous fat in Tibetan sheep fed a low-protein diet.MethodsNinety healthy male Tibetan sheep of...

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Main Authors: Rengeerli Sa, Fengshuo Zhang, Xianhua Zhang, Wei Gao, Yu Zhang, Jiacheng Gan, Shengzhen Hou, Linsheng Gui
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-01-01
Series:Frontiers in Veterinary Science
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Online Access:https://www.frontiersin.org/articles/10.3389/fvets.2024.1528331/full
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author Rengeerli Sa
Fengshuo Zhang
Xianhua Zhang
Wei Gao
Yu Zhang
Jiacheng Gan
Shengzhen Hou
Linsheng Gui
author_facet Rengeerli Sa
Fengshuo Zhang
Xianhua Zhang
Wei Gao
Yu Zhang
Jiacheng Gan
Shengzhen Hou
Linsheng Gui
author_sort Rengeerli Sa
collection DOAJ
description IntroductionThis study employed lipidomics to investigate the effects of varying lysine (Lys)- to-methionine (Met) ratios on the antioxidant capacity, tissue morphology, and fatty acid composition of subcutaneous fat in Tibetan sheep fed a low-protein diet.MethodsNinety healthy male Tibetan sheep of similar body weight were randomly allocated into three groups. These sheep were fed a low-protein diet containing Lys/Met ratios of 1:1, 2:1, and 3:1. Ultra-High Performance Liquid Chromatography–tandem Mass Spectrometry (UHPLC–MS/MS) was employed to explore the changes in various lipid subclasses in subcutaneous adipose tissue. The expression of genes associated with adipogenesis, antioxidant capacity, and fatty acid metabolism was also examined.ResultsThe results indicated that the 1:1 Lys/Met group exhibited significantly higher antioxidant capacity (glutathione peroxidase, GSH-Px), with more orderly adipocyte arrangement, uniform cell size, and a general increase in unsaturated fatty acid levels. Additionally, several lipid molecules associated with the phenotype (Antioxidant index and fatty acid content) were identified, namely, DG(38:3e) + Na, PE(17:1_22:2)-H, PI(17:0_20:3)-H, TG(33:0e) + NH4, Cer(d14:0_17:1) + H, and CL(81:13)-2H. Furthermore, the findings showed that the upregulation of PPARγ, FASN, FAD4, CPT1A, and GPX4 can enhance adipocyte differentiation and lipid accumulation, thereby improving metabolic function in subcutaneous adipose tissue via the regulation of lipid metabolism and oxidative defense mechanisms.DiscussionIn summary, this study provides a theoretical foundation for optimizing precision feeding strategies for Tibetan sheep, offering crucial data to support enhancements in production efficiency and meat quality.
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spelling doaj-art-a3d06c0e02624a65a50f46338f5381ba2025-01-30T17:24:37ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-01-011110.3389/fvets.2024.15283311528331Effects of different Lys/Met ratios on the antioxidant capacity, tissue morphology, and fatty acid composition of subcutaneous fat in Tibetan sheep on low-protein diets: a lipidomic analysisRengeerli SaFengshuo ZhangXianhua ZhangWei GaoYu ZhangJiacheng GanShengzhen HouLinsheng GuiIntroductionThis study employed lipidomics to investigate the effects of varying lysine (Lys)- to-methionine (Met) ratios on the antioxidant capacity, tissue morphology, and fatty acid composition of subcutaneous fat in Tibetan sheep fed a low-protein diet.MethodsNinety healthy male Tibetan sheep of similar body weight were randomly allocated into three groups. These sheep were fed a low-protein diet containing Lys/Met ratios of 1:1, 2:1, and 3:1. Ultra-High Performance Liquid Chromatography–tandem Mass Spectrometry (UHPLC–MS/MS) was employed to explore the changes in various lipid subclasses in subcutaneous adipose tissue. The expression of genes associated with adipogenesis, antioxidant capacity, and fatty acid metabolism was also examined.ResultsThe results indicated that the 1:1 Lys/Met group exhibited significantly higher antioxidant capacity (glutathione peroxidase, GSH-Px), with more orderly adipocyte arrangement, uniform cell size, and a general increase in unsaturated fatty acid levels. Additionally, several lipid molecules associated with the phenotype (Antioxidant index and fatty acid content) were identified, namely, DG(38:3e) + Na, PE(17:1_22:2)-H, PI(17:0_20:3)-H, TG(33:0e) + NH4, Cer(d14:0_17:1) + H, and CL(81:13)-2H. Furthermore, the findings showed that the upregulation of PPARγ, FASN, FAD4, CPT1A, and GPX4 can enhance adipocyte differentiation and lipid accumulation, thereby improving metabolic function in subcutaneous adipose tissue via the regulation of lipid metabolism and oxidative defense mechanisms.DiscussionIn summary, this study provides a theoretical foundation for optimizing precision feeding strategies for Tibetan sheep, offering crucial data to support enhancements in production efficiency and meat quality.https://www.frontiersin.org/articles/10.3389/fvets.2024.1528331/fulllipidomicssubcutaneous fatlow proteinamino acidsnutritional regulation
spellingShingle Rengeerli Sa
Fengshuo Zhang
Xianhua Zhang
Wei Gao
Yu Zhang
Jiacheng Gan
Shengzhen Hou
Linsheng Gui
Effects of different Lys/Met ratios on the antioxidant capacity, tissue morphology, and fatty acid composition of subcutaneous fat in Tibetan sheep on low-protein diets: a lipidomic analysis
Frontiers in Veterinary Science
lipidomics
subcutaneous fat
low protein
amino acids
nutritional regulation
title Effects of different Lys/Met ratios on the antioxidant capacity, tissue morphology, and fatty acid composition of subcutaneous fat in Tibetan sheep on low-protein diets: a lipidomic analysis
title_full Effects of different Lys/Met ratios on the antioxidant capacity, tissue morphology, and fatty acid composition of subcutaneous fat in Tibetan sheep on low-protein diets: a lipidomic analysis
title_fullStr Effects of different Lys/Met ratios on the antioxidant capacity, tissue morphology, and fatty acid composition of subcutaneous fat in Tibetan sheep on low-protein diets: a lipidomic analysis
title_full_unstemmed Effects of different Lys/Met ratios on the antioxidant capacity, tissue morphology, and fatty acid composition of subcutaneous fat in Tibetan sheep on low-protein diets: a lipidomic analysis
title_short Effects of different Lys/Met ratios on the antioxidant capacity, tissue morphology, and fatty acid composition of subcutaneous fat in Tibetan sheep on low-protein diets: a lipidomic analysis
title_sort effects of different lys met ratios on the antioxidant capacity tissue morphology and fatty acid composition of subcutaneous fat in tibetan sheep on low protein diets a lipidomic analysis
topic lipidomics
subcutaneous fat
low protein
amino acids
nutritional regulation
url https://www.frontiersin.org/articles/10.3389/fvets.2024.1528331/full
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