Pharmacological properties of biomimetic synthesized silver nanoparticles from endophytic fungus Coniothyrium chaingmaiense: KUMBMDBT-25
Abstract In this study, the endophytic fungus Coniothyrium chaingmaiense-KUMBMDBT-25 was isolated from the healthy stem of Euphorbia tirucalli, mass cultivated by submerged fermentation, and extracted using ethyl acetate as a solvent. The extract was subjected to GC-MS analysis. The synthesized Con-...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-024-76475-x |
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| Summary: | Abstract In this study, the endophytic fungus Coniothyrium chaingmaiense-KUMBMDBT-25 was isolated from the healthy stem of Euphorbia tirucalli, mass cultivated by submerged fermentation, and extracted using ethyl acetate as a solvent. The extract was subjected to GC-MS analysis. The synthesized Con-AgNPs were characterized through various bioanalytical methods. The synthesis was confirmed by Bio- spectrophotometry, which showed an absorption peak at 404 nm. FTIR analysis verified the reduction and capping of Con-AgNPs, displaying peaks corresponding to various functional groups. SEM-EDAX and HR-TEM examinations revealed that the Con-AgNPs were spherical, and EDAX analysis confirmed the presence of silver atoms at 3 keV. XRD studies revealed the crystalline structure of Con-AgNPs. DLS and Zeta potential tests determined the size and stability of the synthesized Con-AgNPs, which were 65.81 nm. The Con-AgNPs demonstrated strong antibacterial activity against P. aeruginosa (14.06 ± 0.11 mm, 10 mg/mL) and effective antifungal activity against A. flavus (13.03 ± 0.05 mm, 10 mg/mL). Con-AgNPs exhibited notable biological attributes, including a cytotoxic effect of up to 38.82% and 19.15% at 200 µg/mL in an MTT assay measuring cell viability. Additionally, the nanoparticles demonstrated significant anti-inflammatory effects in both in vitro and in vivo studies, validating the biological and pharmacological potential of Con-AgNPs. |
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| ISSN: | 2045-2322 |