A novel theoretical framework for simultaneous measurement of excitatory and inhibitory conductances.
The firing of neurons throughout the brain is determined by the precise relations between excitatory and inhibitory inputs, and disruption of their balance underlies many psychiatric diseases. Whether or not these inputs covary over time or between repeated stimuli remains unclear due to the lack of...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-12-01
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| Series: | PLoS Computational Biology |
| Online Access: | https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.1009725&type=printable |
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| author | Daniel Müller-Komorowska Ana Parabucki Gal Elyasaf Yonatan Katz Heinz Beck Ilan Lampl |
| author_facet | Daniel Müller-Komorowska Ana Parabucki Gal Elyasaf Yonatan Katz Heinz Beck Ilan Lampl |
| author_sort | Daniel Müller-Komorowska |
| collection | DOAJ |
| description | The firing of neurons throughout the brain is determined by the precise relations between excitatory and inhibitory inputs, and disruption of their balance underlies many psychiatric diseases. Whether or not these inputs covary over time or between repeated stimuli remains unclear due to the lack of experimental methods for measuring both inputs simultaneously. We developed a new analytical framework for instantaneous and simultaneous measurements of both the excitatory and inhibitory neuronal inputs during a single trial under current clamp recording. This can be achieved by injecting a current composed of two high frequency sinusoidal components followed by analytical extraction of the conductances. We demonstrate the ability of this method to measure both inputs in a single trial under realistic recording constraints and from morphologically realistic CA1 pyramidal model cells. Future experimental implementation of our new method will facilitate the understanding of fundamental questions about the health and disease of the nervous system. |
| format | Article |
| id | doaj-art-a3c7c3abde394a2a8feed236bd66699b |
| institution | DOAJ |
| issn | 1553-734X 1553-7358 |
| language | English |
| publishDate | 2021-12-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Computational Biology |
| spelling | doaj-art-a3c7c3abde394a2a8feed236bd66699b2025-08-20T02:46:24ZengPublic Library of Science (PLoS)PLoS Computational Biology1553-734X1553-73582021-12-011712e100972510.1371/journal.pcbi.1009725A novel theoretical framework for simultaneous measurement of excitatory and inhibitory conductances.Daniel Müller-KomorowskaAna ParabuckiGal ElyasafYonatan KatzHeinz BeckIlan LamplThe firing of neurons throughout the brain is determined by the precise relations between excitatory and inhibitory inputs, and disruption of their balance underlies many psychiatric diseases. Whether or not these inputs covary over time or between repeated stimuli remains unclear due to the lack of experimental methods for measuring both inputs simultaneously. We developed a new analytical framework for instantaneous and simultaneous measurements of both the excitatory and inhibitory neuronal inputs during a single trial under current clamp recording. This can be achieved by injecting a current composed of two high frequency sinusoidal components followed by analytical extraction of the conductances. We demonstrate the ability of this method to measure both inputs in a single trial under realistic recording constraints and from morphologically realistic CA1 pyramidal model cells. Future experimental implementation of our new method will facilitate the understanding of fundamental questions about the health and disease of the nervous system.https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.1009725&type=printable |
| spellingShingle | Daniel Müller-Komorowska Ana Parabucki Gal Elyasaf Yonatan Katz Heinz Beck Ilan Lampl A novel theoretical framework for simultaneous measurement of excitatory and inhibitory conductances. PLoS Computational Biology |
| title | A novel theoretical framework for simultaneous measurement of excitatory and inhibitory conductances. |
| title_full | A novel theoretical framework for simultaneous measurement of excitatory and inhibitory conductances. |
| title_fullStr | A novel theoretical framework for simultaneous measurement of excitatory and inhibitory conductances. |
| title_full_unstemmed | A novel theoretical framework for simultaneous measurement of excitatory and inhibitory conductances. |
| title_short | A novel theoretical framework for simultaneous measurement of excitatory and inhibitory conductances. |
| title_sort | novel theoretical framework for simultaneous measurement of excitatory and inhibitory conductances |
| url | https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.1009725&type=printable |
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