Automated Mycoplasma genitalium molecular macrolide resistance detection and nucleic acid target semi-quantitation: patient demographic considerations
ABSTRACT Recent work has optimized parameters of a real-time reverse transcriptase PCR-based laboratory-developed test on the Panther Fusion system which detects Mycoplasma genitalium-specific macrolide resistance-associated mutations LDT (MRM-LDT) from primary swab and urine specimens. In this stud...
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American Society for Microbiology
2025-07-01
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| Series: | Microbiology Spectrum |
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| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.00738-25 |
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| author | Josephine Moore Trinity Krueger Amanda Zapp Stephen C. Lavey Kimber L. Munson Irene A. Stafford Michael E. Newcomb Brian Mustanski Erik Munson |
| author_facet | Josephine Moore Trinity Krueger Amanda Zapp Stephen C. Lavey Kimber L. Munson Irene A. Stafford Michael E. Newcomb Brian Mustanski Erik Munson |
| author_sort | Josephine Moore |
| collection | DOAJ |
| description | ABSTRACT Recent work has optimized parameters of a real-time reverse transcriptase PCR-based laboratory-developed test on the Panther Fusion system which detects Mycoplasma genitalium-specific macrolide resistance-associated mutations LDT (MRM-LDT) from primary swab and urine specimens. In this study, MRM-LDT was applied to a large multi-demographic study set to further characterize M. genitalium resistance in the United States. A total of 2,145 primary clinical specimens testing positive for M. genitalium 16S rRNA by transcription-mediated amplification (TMA) were initially titered by the same assay using serial 10-fold dilutions to determine relative target nucleic acid burden. Specimens were then processed for MRM-LDT. Findings were stratified by men who have sex with men (MSM; n = 3 settings), community care (n = 2), and university student (n = 3) populations. The mean log10 target nucleic acid titer of a TMA-positive specimen was 3.46 (median 3; range 0–10). A 43.2% MRM-LDT detection rate was found in specimens derived from community care settings. Analogous assessments of university student and MSM demographics revealed 60.8% and 62.0% detection, respectively (P ≤ 0.0004 versus community care). Within the university student demographic, differences existed in target nucleic acid titer for two settings (P = 0.01); similar differences were encountered between a local MSM cohort and two that were nationally based (P ≤ 0.01). Within the university cohort, MRM-LDT detection rate was increased in symptomatic patients (P = 0.005). M. genitalium macrolide resistance rates among multiple demographics, as determined by MRM-LDT, are high in the United States and are consistent with target nucleic acid burden within the primary specimen. However, caveats experienced within subgroupings of these demographics support reflex MRM-LDT on M. genitalium-positive specimens.IMPORTANCEData generated from a high-throughput, automated system and presented in this report expand upon knowledge of Mycoplasma genitalium-specific macrolide resistance in the United States and may further inform providers on population- or demographic-based considerations for macrolide resistance mutation determination in M. genitalium. |
| format | Article |
| id | doaj-art-a3ba4a90485641a2b4f1bc2ba48d5e41 |
| institution | Kabale University |
| issn | 2165-0497 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | American Society for Microbiology |
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| series | Microbiology Spectrum |
| spelling | doaj-art-a3ba4a90485641a2b4f1bc2ba48d5e412025-08-20T03:28:26ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972025-07-0113710.1128/spectrum.00738-25Automated Mycoplasma genitalium molecular macrolide resistance detection and nucleic acid target semi-quantitation: patient demographic considerationsJosephine Moore0Trinity Krueger1Amanda Zapp2Stephen C. Lavey3Kimber L. Munson4Irene A. Stafford5Michael E. Newcomb6Brian Mustanski7Erik Munson8Department of Medical Laboratory Science, Marquette University, Milwaukee, Wisconsin, USADepartment of Medical Laboratory Science, Marquette University, Milwaukee, Wisconsin, USADepartment of Medical Laboratory Science, Marquette University, Milwaukee, Wisconsin, USALoyola University Parkinson School of Health Sciences and Public Health, Maywood, Illinois, USADepartment of Medical Laboratory Science, Marquette University, Milwaukee, Wisconsin, USADepartment of Obstetrics and Gynecology, McGovern Medical School at UTHealth, Houston, Texas, USADivision of Infectious Diseases, Northwestern University, Chicago, Illinois, USADivision of Infectious Diseases, Northwestern University, Chicago, Illinois, USAWisconsin Clinical Laboratory Network Laboratory Technical Advisory Group, Madison, Wisconsin, USAABSTRACT Recent work has optimized parameters of a real-time reverse transcriptase PCR-based laboratory-developed test on the Panther Fusion system which detects Mycoplasma genitalium-specific macrolide resistance-associated mutations LDT (MRM-LDT) from primary swab and urine specimens. In this study, MRM-LDT was applied to a large multi-demographic study set to further characterize M. genitalium resistance in the United States. A total of 2,145 primary clinical specimens testing positive for M. genitalium 16S rRNA by transcription-mediated amplification (TMA) were initially titered by the same assay using serial 10-fold dilutions to determine relative target nucleic acid burden. Specimens were then processed for MRM-LDT. Findings were stratified by men who have sex with men (MSM; n = 3 settings), community care (n = 2), and university student (n = 3) populations. The mean log10 target nucleic acid titer of a TMA-positive specimen was 3.46 (median 3; range 0–10). A 43.2% MRM-LDT detection rate was found in specimens derived from community care settings. Analogous assessments of university student and MSM demographics revealed 60.8% and 62.0% detection, respectively (P ≤ 0.0004 versus community care). Within the university student demographic, differences existed in target nucleic acid titer for two settings (P = 0.01); similar differences were encountered between a local MSM cohort and two that were nationally based (P ≤ 0.01). Within the university cohort, MRM-LDT detection rate was increased in symptomatic patients (P = 0.005). M. genitalium macrolide resistance rates among multiple demographics, as determined by MRM-LDT, are high in the United States and are consistent with target nucleic acid burden within the primary specimen. However, caveats experienced within subgroupings of these demographics support reflex MRM-LDT on M. genitalium-positive specimens.IMPORTANCEData generated from a high-throughput, automated system and presented in this report expand upon knowledge of Mycoplasma genitalium-specific macrolide resistance in the United States and may further inform providers on population- or demographic-based considerations for macrolide resistance mutation determination in M. genitalium.https://journals.asm.org/doi/10.1128/spectrum.00738-25Mycoplasmoides genitaliumtranscription-mediated amplificationmacrolide resistancesemi-quantitation |
| spellingShingle | Josephine Moore Trinity Krueger Amanda Zapp Stephen C. Lavey Kimber L. Munson Irene A. Stafford Michael E. Newcomb Brian Mustanski Erik Munson Automated Mycoplasma genitalium molecular macrolide resistance detection and nucleic acid target semi-quantitation: patient demographic considerations Microbiology Spectrum Mycoplasmoides genitalium transcription-mediated amplification macrolide resistance semi-quantitation |
| title | Automated Mycoplasma genitalium molecular macrolide resistance detection and nucleic acid target semi-quantitation: patient demographic considerations |
| title_full | Automated Mycoplasma genitalium molecular macrolide resistance detection and nucleic acid target semi-quantitation: patient demographic considerations |
| title_fullStr | Automated Mycoplasma genitalium molecular macrolide resistance detection and nucleic acid target semi-quantitation: patient demographic considerations |
| title_full_unstemmed | Automated Mycoplasma genitalium molecular macrolide resistance detection and nucleic acid target semi-quantitation: patient demographic considerations |
| title_short | Automated Mycoplasma genitalium molecular macrolide resistance detection and nucleic acid target semi-quantitation: patient demographic considerations |
| title_sort | automated mycoplasma genitalium molecular macrolide resistance detection and nucleic acid target semi quantitation patient demographic considerations |
| topic | Mycoplasmoides genitalium transcription-mediated amplification macrolide resistance semi-quantitation |
| url | https://journals.asm.org/doi/10.1128/spectrum.00738-25 |
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