Extraction and composition analysis of flavonoids in peony seed shells

Objective: To extract natural flavonoid compounds (MDZK) from peony seed husks for composition analysis and antioxidant research. Methods: The total flavonoids of peony seed shell were extracted by solvent reflux extraction method. The ultraviolet-visible spectrophotometer method was used to determi...

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Main Authors: ZOU Ping, ZHANG Ying-yang, ZOU Lin-ling
Format: Article
Language:English
Published: The Editorial Office of Food and Machinery 2023-06-01
Series:Shipin yu jixie
Subjects:
Online Access:http://www.ifoodmm.com/spyjxen/article/abstract/20230408
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author ZOU Ping
ZHANG Ying-yang
ZOU Lin-ling
author_facet ZOU Ping
ZHANG Ying-yang
ZOU Lin-ling
author_sort ZOU Ping
collection DOAJ
description Objective: To extract natural flavonoid compounds (MDZK) from peony seed husks for composition analysis and antioxidant research. Methods: The total flavonoids of peony seed shell were extracted by solvent reflux extraction method. The ultraviolet-visible spectrophotometer method was used to determine the MDZK content and antioxidant properties; The total flavonoids extracted were analyzed by LC-MS method. Results: When the solvent was 70% ethanol, the extraction temperature was 70 ℃, the feed-to-liquid ratio was 1∶25 (g/mL), and the extraction time was 60 min, the MDZK yield was the highest, which was (10.54±0.13)%. When the concentration of MDZK reached 120 μg/mL, the highest clearance rates of DPPH radicals, O<sub>2</sub> radicals and ·OH radicals were 58.54%, 55.72% and 49.07%, respectively, which showed that MDZK had certain antioxidant effects and good effects. More than 20 flavonoid compounds and their derivatives were identified by comparison with the database, of which the most abundant content were Luteoloside, Eriodictyol Kaempfero, Apigenin and Neohesperidin. Conclusion: The flavonoid compounds in the shell of peony seeds have good antioxidant activity and can be used as antioxidant products in food industry or medicine, and have wide application prospects.
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spelling doaj-art-a3b6e8d6b02f46dc8513a3f59cf16db52025-08-20T02:21:49ZengThe Editorial Office of Food and MachineryShipin yu jixie1003-57882023-06-01394445010.13652/j.spjx.1003.5788.2022.80598Extraction and composition analysis of flavonoids in peony seed shellsZOU Ping0ZHANG Ying-yang1ZOU Lin-ling2 School of Food, Changzhou University, Changzhou, Jiangsu 213164 , China School of Food, Changzhou University, Changzhou, Jiangsu 213164 , China School of Food, Changzhou University, Changzhou, Jiangsu 213164 , China Objective: To extract natural flavonoid compounds (MDZK) from peony seed husks for composition analysis and antioxidant research. Methods: The total flavonoids of peony seed shell were extracted by solvent reflux extraction method. The ultraviolet-visible spectrophotometer method was used to determine the MDZK content and antioxidant properties; The total flavonoids extracted were analyzed by LC-MS method. Results: When the solvent was 70% ethanol, the extraction temperature was 70 ℃, the feed-to-liquid ratio was 1∶25 (g/mL), and the extraction time was 60 min, the MDZK yield was the highest, which was (10.54±0.13)%. When the concentration of MDZK reached 120 μg/mL, the highest clearance rates of DPPH radicals, O<sub>2</sub> radicals and ·OH radicals were 58.54%, 55.72% and 49.07%, respectively, which showed that MDZK had certain antioxidant effects and good effects. More than 20 flavonoid compounds and their derivatives were identified by comparison with the database, of which the most abundant content were Luteoloside, Eriodictyol Kaempfero, Apigenin and Neohesperidin. Conclusion: The flavonoid compounds in the shell of peony seeds have good antioxidant activity and can be used as antioxidant products in food industry or medicine, and have wide application prospects.http://www.ifoodmm.com/spyjxen/article/abstract/20230408 peony seed shell flavonoids antioxidant activity lc-ms
spellingShingle ZOU Ping
ZHANG Ying-yang
ZOU Lin-ling
Extraction and composition analysis of flavonoids in peony seed shells
Shipin yu jixie
peony seed shell
flavonoids
antioxidant activity
lc-ms
title Extraction and composition analysis of flavonoids in peony seed shells
title_full Extraction and composition analysis of flavonoids in peony seed shells
title_fullStr Extraction and composition analysis of flavonoids in peony seed shells
title_full_unstemmed Extraction and composition analysis of flavonoids in peony seed shells
title_short Extraction and composition analysis of flavonoids in peony seed shells
title_sort extraction and composition analysis of flavonoids in peony seed shells
topic peony seed shell
flavonoids
antioxidant activity
lc-ms
url http://www.ifoodmm.com/spyjxen/article/abstract/20230408
work_keys_str_mv AT zouping extractionandcompositionanalysisofflavonoidsinpeonyseedshells
AT zhangyingyang extractionandcompositionanalysisofflavonoidsinpeonyseedshells
AT zoulinling extractionandcompositionanalysisofflavonoidsinpeonyseedshells