SPECTRAL PROPERTIES OF TWO WATER-SOLUBLE MELANINS
Aim. The work was purposed to study the spectral properties of two types of water-soluble melanins to clarify the nature of the optical absorption and emission centers in them. Materials and methods. Studied are the spectral properties of two types of water-soluble melanins: melanin obtained fro...
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| Format: | Article |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2024-12-01
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| Series: | Biotechnologia Acta |
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| Online Access: | https://biotechnology.kiev.ua/images/BTA/2024/6_2024/Kravchenko_6_2024.pdf |
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| author | V.M. Kravchenko T.V. Beregova M.Yu. Lositskyi T.O. Kondratyuk S.A. Davydenko E.S. Davydenko S.S. Davydenko L.P. Buchatskyi V.M. Yashchuk |
| author_facet | V.M. Kravchenko T.V. Beregova M.Yu. Lositskyi T.O. Kondratyuk S.A. Davydenko E.S. Davydenko S.S. Davydenko L.P. Buchatskyi V.M. Yashchuk |
| author_sort | V.M. Kravchenko |
| collection | DOAJ |
| description | Aim. The work was purposed to study the spectral properties of two types of water-soluble melanins to clarify the nature of the optical absorption and emission centers in them.
Materials and methods. Studied are the spectral properties of two types of water-soluble melanins: melanin obtained from black yeast-like fungi Pseudonadsoniella brunnea 470 FCKU, dissolved in water with pH 11, and plant melanin obtained from black tea according to German patent DE102004003801A1, which is added to drinking water for medicinal purposes. In the spectral region of 200–900 nm, optical absorption spectra at room temperature, fluorescence spectra, and fluorescence excitation at room temperature and liquid nitrogen temperature (78 K) are measured.
Results. The optical density of both studied samples monotonically decreases with increasing radiation wavelength. Fluorescence spectra represent one broadband, the peak position of which depends on the excitation wavelength. The fluorescence excitation spectrum consists of at least two bands and does not coincide with the absorption spectrum.
Conclusions. The shape of the absorption spectra may indicate that the aqueous solutions of melanins under study contain many absorption centers with different resonance frequencies. Fluorescence spectra represent a superposition of several elementary bands with varying spectra of excitation. The different excitation spectra corresponding to fluorescence at different wavelengths most likely indicate that each of the melanin solutions under study contains several of its species. The spectral range of absorption, as well as the low fluorescence intensity, make further studies of water-soluble melanins as components of complex nanosystems for photoacoustics and photothermal therapy promising. The use of producer microorganisms for melanin production provides an economically viable biotechnological process. |
| format | Article |
| id | doaj-art-a3a263a1ce984eea9e9630c8696d6bee |
| institution | DOAJ |
| issn | 2410-7751 2410-776X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj-art-a3a263a1ce984eea9e9630c8696d6bee2025-08-20T02:47:58ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2024-12-01176283410.15407/biotech17.06.028SPECTRAL PROPERTIES OF TWO WATER-SOLUBLE MELANINSV.M. Kravchenko0T.V. Beregova1M.Yu. Lositskyi2T.O. Kondratyuk3S.A. Davydenko4E.S. Davydenko5S.S. Davydenko6 L.P. Buchatskyi7 V.M. Yashchuk8Taras Shevchenko National University of Kyiv, UkraineTaras Shevchenko National University of Kyiv, UkraineTaras Shevchenko National University of Kyiv, UkraineTaras Shevchenko National University of Kyiv, UkraineLLC “Plant Polymer Melanin”, Kyiv, UkraineLLC “Plant Polymer Melanin”, Kyiv, UkraineLLC “Plant Polymer Melanin”, Kyiv, UkraineTaras Shevchenko National University of Kyiv, UkraineTaras Shevchenko National University of Kyiv, UkraineAim. The work was purposed to study the spectral properties of two types of water-soluble melanins to clarify the nature of the optical absorption and emission centers in them. Materials and methods. Studied are the spectral properties of two types of water-soluble melanins: melanin obtained from black yeast-like fungi Pseudonadsoniella brunnea 470 FCKU, dissolved in water with pH 11, and plant melanin obtained from black tea according to German patent DE102004003801A1, which is added to drinking water for medicinal purposes. In the spectral region of 200–900 nm, optical absorption spectra at room temperature, fluorescence spectra, and fluorescence excitation at room temperature and liquid nitrogen temperature (78 K) are measured. Results. The optical density of both studied samples monotonically decreases with increasing radiation wavelength. Fluorescence spectra represent one broadband, the peak position of which depends on the excitation wavelength. The fluorescence excitation spectrum consists of at least two bands and does not coincide with the absorption spectrum. Conclusions. The shape of the absorption spectra may indicate that the aqueous solutions of melanins under study contain many absorption centers with different resonance frequencies. Fluorescence spectra represent a superposition of several elementary bands with varying spectra of excitation. The different excitation spectra corresponding to fluorescence at different wavelengths most likely indicate that each of the melanin solutions under study contains several of its species. The spectral range of absorption, as well as the low fluorescence intensity, make further studies of water-soluble melanins as components of complex nanosystems for photoacoustics and photothermal therapy promising. The use of producer microorganisms for melanin production provides an economically viable biotechnological process.https://biotechnology.kiev.ua/images/BTA/2024/6_2024/Kravchenko_6_2024.pdfmelaninaqueous solutionoptical absorptionfluorescencespectrum. |
| spellingShingle | V.M. Kravchenko T.V. Beregova M.Yu. Lositskyi T.O. Kondratyuk S.A. Davydenko E.S. Davydenko S.S. Davydenko L.P. Buchatskyi V.M. Yashchuk SPECTRAL PROPERTIES OF TWO WATER-SOLUBLE MELANINS Biotechnologia Acta melanin aqueous solution optical absorption fluorescence spectrum. |
| title | SPECTRAL PROPERTIES OF TWO WATER-SOLUBLE MELANINS |
| title_full | SPECTRAL PROPERTIES OF TWO WATER-SOLUBLE MELANINS |
| title_fullStr | SPECTRAL PROPERTIES OF TWO WATER-SOLUBLE MELANINS |
| title_full_unstemmed | SPECTRAL PROPERTIES OF TWO WATER-SOLUBLE MELANINS |
| title_short | SPECTRAL PROPERTIES OF TWO WATER-SOLUBLE MELANINS |
| title_sort | spectral properties of two water soluble melanins |
| topic | melanin aqueous solution optical absorption fluorescence spectrum. |
| url | https://biotechnology.kiev.ua/images/BTA/2024/6_2024/Kravchenko_6_2024.pdf |
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