Impact of Interleukin-10 Promoter Region Polymorphisms on Recurrent Miscarriage: A Case–Control Approach
Background: Recurrent miscarriage (RM), defined as two or more consecutive miscarriages prior to the 20th week of gestation is characterised by multifactorial aetiology. The prevalence of RM varies from 0.8% to 13.5% amongst women of reproductive age. The aetiological basis of RM has been traced to...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-04-01
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| Series: | Journal of Human Reproductive Sciences |
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| Online Access: | https://journals.lww.com/10.4103/jhrs.jhrs_43_23 |
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| author | Neha Sudhir Mandeep Kaur Sukhjashanpreet Singh Archana Beri Tajinder Kaur Anupam Kaur |
| author_facet | Neha Sudhir Mandeep Kaur Sukhjashanpreet Singh Archana Beri Tajinder Kaur Anupam Kaur |
| author_sort | Neha Sudhir |
| collection | DOAJ |
| description | Background:
Recurrent miscarriage (RM), defined as two or more consecutive miscarriages prior to the 20th week of gestation is characterised by multifactorial aetiology. The prevalence of RM varies from 0.8% to 13.5% amongst women of reproductive age. The aetiological basis of RM has been traced to chromosomal, anatomic, hormonal and immunologic factors while half of the cases remain idiopathic.
Aims:
This study aimed to investigate the association of interleukin-10 (IL-10) polymorphisms with RM amongst the Indian population.
Settings and Design:
The present study included a total of 414 individuals including RM women (n = 199) with two or more pregnancy losses and healthy women (n = 215) without any previous history of pregnancy loss were taken as the control group.
Materials and Methods:
Demographic features and reproductive history of women with RM and healthy women were taken. Genotype analysis of IL-10 polymorphisms rs1800872 and rs1800896 was performed using the polymerase chain reaction (PCR) restriction fragment length polymorphism and amplification mutation refractory system PCR, respectively.
Statistical Analysis Used:
Student's t-test was used to compare the demographic features and reproductive history amongst both groups. Pearson's Chi-square was used to calculate the Hardy–Weinberg equilibrium, allelic and genotypic frequencies. All the statistical analyses were performed using the SPSS (version 21, IBM SPSS, NY, USA).
Results:
Our results suggested that the genotypic and allelic frequency of rs1800872 polymorphism did not differ significantly between RM cases and control women (P = 0.07 and P = 0.23, respectively). The GG genotype (P = 0.007) and G allele (P = 0.003) of rs1800896 were significantly associated with an increased risk of RM. A statistically significant difference was also found for the distribution of genetic models (dominant and co-dominant model) between both groups for rs1800896. However, haplotype analysis revealed that none of the haplotypes provides a risk for the progression of RM.
Conclusion:
The study is the first of its kind from our region and provides baseline data on the genetics of RM. |
| format | Article |
| id | doaj-art-a3a246d588574c0e98984b8187cdcd58 |
| institution | Kabale University |
| issn | 0974-1208 1998-4766 |
| language | English |
| publishDate | 2023-04-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Human Reproductive Sciences |
| spelling | doaj-art-a3a246d588574c0e98984b8187cdcd582025-02-10T07:44:28ZengWolters Kluwer Medknow PublicationsJournal of Human Reproductive Sciences0974-12081998-47662023-04-0116215616510.4103/jhrs.jhrs_43_23Impact of Interleukin-10 Promoter Region Polymorphisms on Recurrent Miscarriage: A Case–Control ApproachNeha SudhirMandeep KaurSukhjashanpreet SinghArchana BeriTajinder KaurAnupam KaurBackground: Recurrent miscarriage (RM), defined as two or more consecutive miscarriages prior to the 20th week of gestation is characterised by multifactorial aetiology. The prevalence of RM varies from 0.8% to 13.5% amongst women of reproductive age. The aetiological basis of RM has been traced to chromosomal, anatomic, hormonal and immunologic factors while half of the cases remain idiopathic. Aims: This study aimed to investigate the association of interleukin-10 (IL-10) polymorphisms with RM amongst the Indian population. Settings and Design: The present study included a total of 414 individuals including RM women (n = 199) with two or more pregnancy losses and healthy women (n = 215) without any previous history of pregnancy loss were taken as the control group. Materials and Methods: Demographic features and reproductive history of women with RM and healthy women were taken. Genotype analysis of IL-10 polymorphisms rs1800872 and rs1800896 was performed using the polymerase chain reaction (PCR) restriction fragment length polymorphism and amplification mutation refractory system PCR, respectively. Statistical Analysis Used: Student's t-test was used to compare the demographic features and reproductive history amongst both groups. Pearson's Chi-square was used to calculate the Hardy–Weinberg equilibrium, allelic and genotypic frequencies. All the statistical analyses were performed using the SPSS (version 21, IBM SPSS, NY, USA). Results: Our results suggested that the genotypic and allelic frequency of rs1800872 polymorphism did not differ significantly between RM cases and control women (P = 0.07 and P = 0.23, respectively). The GG genotype (P = 0.007) and G allele (P = 0.003) of rs1800896 were significantly associated with an increased risk of RM. A statistically significant difference was also found for the distribution of genetic models (dominant and co-dominant model) between both groups for rs1800896. However, haplotype analysis revealed that none of the haplotypes provides a risk for the progression of RM. Conclusion: The study is the first of its kind from our region and provides baseline data on the genetics of RM.https://journals.lww.com/10.4103/jhrs.jhrs_43_23cytokineinterleukin-10polymorphismsrecurrent miscarriage |
| spellingShingle | Neha Sudhir Mandeep Kaur Sukhjashanpreet Singh Archana Beri Tajinder Kaur Anupam Kaur Impact of Interleukin-10 Promoter Region Polymorphisms on Recurrent Miscarriage: A Case–Control Approach Journal of Human Reproductive Sciences cytokine interleukin-10 polymorphisms recurrent miscarriage |
| title | Impact of Interleukin-10 Promoter Region Polymorphisms on Recurrent Miscarriage: A Case–Control Approach |
| title_full | Impact of Interleukin-10 Promoter Region Polymorphisms on Recurrent Miscarriage: A Case–Control Approach |
| title_fullStr | Impact of Interleukin-10 Promoter Region Polymorphisms on Recurrent Miscarriage: A Case–Control Approach |
| title_full_unstemmed | Impact of Interleukin-10 Promoter Region Polymorphisms on Recurrent Miscarriage: A Case–Control Approach |
| title_short | Impact of Interleukin-10 Promoter Region Polymorphisms on Recurrent Miscarriage: A Case–Control Approach |
| title_sort | impact of interleukin 10 promoter region polymorphisms on recurrent miscarriage a case control approach |
| topic | cytokine interleukin-10 polymorphisms recurrent miscarriage |
| url | https://journals.lww.com/10.4103/jhrs.jhrs_43_23 |
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