Isolation and characterization of plant growth promoting rhizobacteria from cacti root under drought condition
Plant growth-promoting rhizobia (PGPR) helps plants grow and develop by protecting them from abiotic and biotic stresses, increasing the synthesis of chemicals that promote growth, and enabling the uptake of nutrients. Drought is one of the biggest problems throughout the world. The search for novel...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Current Research in Microbial Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666517424001020 |
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| author | Kumar Shreshtha Aman Prakash Prashant Kumar Pandey Arun Kumar Pal Jyotsna Singh Pooja Tripathi Debasis Mitra Durgesh Kumar Jaiswal Sergio de los Santos-Villalobos Vijay Tripathi |
| author_facet | Kumar Shreshtha Aman Prakash Prashant Kumar Pandey Arun Kumar Pal Jyotsna Singh Pooja Tripathi Debasis Mitra Durgesh Kumar Jaiswal Sergio de los Santos-Villalobos Vijay Tripathi |
| author_sort | Kumar Shreshtha |
| collection | DOAJ |
| description | Plant growth-promoting rhizobia (PGPR) helps plants grow and develop by protecting them from abiotic and biotic stresses, increasing the synthesis of chemicals that promote growth, and enabling the uptake of nutrients. Drought is one of the biggest problems throughout the world. The search for novel and efficient drought-resistant microorganisms that reduce the adverse effects executed by drought is a significant alternative. This study aimed to isolate and characterize PGPR strains from the Opuntia Ficus-Indica cactus plant's rhizosphere, cultivated in the semi-arid Shankargarh district of Uttar Pradesh, India. Tests for plant growth-promoting activity, such as the generation of indole acetic acid (IAA), phosphate solubilization, ammonia, carboxymethyl cellulase, and protease activity, were performed on all bacterial isolates. There were 246 bacterial strains isolated from the rhizospheric zone, and only 16.6 % showed drought resistance and various plant growth-promoting traits. The Bacillus sp. strain promoted the growth promotion of Capsicum annum L. under water stress (30 % field capacity). Additionally, Bacillus sp. isolates, with their potential for drought tolerance and plant growth promotion, could be applied in sustainable agriculture to enhance crop yield and resilience to water scarcity. |
| format | Article |
| id | doaj-art-a3a2106968db43df92bc35372b6b0bfe |
| institution | Kabale University |
| issn | 2666-5174 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Microbial Sciences |
| spelling | doaj-art-a3a2106968db43df92bc35372b6b0bfe2025-08-20T03:46:49ZengElsevierCurrent Research in Microbial Sciences2666-51742025-01-01810031910.1016/j.crmicr.2024.100319Isolation and characterization of plant growth promoting rhizobacteria from cacti root under drought conditionKumar Shreshtha0Aman Prakash1Prashant Kumar Pandey2Arun Kumar Pal3Jyotsna Singh4Pooja Tripathi5Debasis Mitra6Durgesh Kumar Jaiswal7Sergio de los Santos-Villalobos8Vijay Tripathi9Department of Molecular and Cellular Engineering, Jacob Institute of Biotechnology and Bioengineering, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, U.P., 211007, IndiaDepartment of Molecular and Cellular Engineering, Jacob Institute of Biotechnology and Bioengineering, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, U.P., 211007, IndiaDepartment of Molecular and Cellular Engineering, Jacob Institute of Biotechnology and Bioengineering, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, U.P., 211007, IndiaDepartment of Molecular and Cellular Engineering, Jacob Institute of Biotechnology and Bioengineering, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, U.P., 211007, IndiaDepartment of Molecular and Cellular Engineering, Jacob Institute of Biotechnology and Bioengineering, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, U.P., 211007, IndiaDepartment of Computational Biology and Bioinformatics, Jacob Institute of Biotechnology and Bioengineering, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, U.P., 211007, IndiaDepartment of Microbiology, Graphic Era Deemed to be University, Clement Town, Dehradun, U.K., 248002, IndiaDepartment of Biotechnology, Graphic Era Deemed to be University, Clement Town, Dehradun, U.K., 248002, IndiaInstituto Tecnológico de Sonora, 5 de febrero 818 sur, 85000 Ciudad Obregón, Sonora, MexicoDepartment of Molecular and Cellular Engineering, Jacob Institute of Biotechnology and Bioengineering, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, U.P., 211007, India; Department of Microbiology, Graphic Era Deemed to be University, Clement Town, Dehradun, U.K., 248002, India; Corresponding author at: Department of Microbiology, Graphic Era Deemed to be University, Clement Town, Dehradun, U.K., 248002, India.Plant growth-promoting rhizobia (PGPR) helps plants grow and develop by protecting them from abiotic and biotic stresses, increasing the synthesis of chemicals that promote growth, and enabling the uptake of nutrients. Drought is one of the biggest problems throughout the world. The search for novel and efficient drought-resistant microorganisms that reduce the adverse effects executed by drought is a significant alternative. This study aimed to isolate and characterize PGPR strains from the Opuntia Ficus-Indica cactus plant's rhizosphere, cultivated in the semi-arid Shankargarh district of Uttar Pradesh, India. Tests for plant growth-promoting activity, such as the generation of indole acetic acid (IAA), phosphate solubilization, ammonia, carboxymethyl cellulase, and protease activity, were performed on all bacterial isolates. There were 246 bacterial strains isolated from the rhizospheric zone, and only 16.6 % showed drought resistance and various plant growth-promoting traits. The Bacillus sp. strain promoted the growth promotion of Capsicum annum L. under water stress (30 % field capacity). Additionally, Bacillus sp. isolates, with their potential for drought tolerance and plant growth promotion, could be applied in sustainable agriculture to enhance crop yield and resilience to water scarcity.http://www.sciencedirect.com/science/article/pii/S2666517424001020CactiDroughtPlant-associated bacteriaPlant growth-promoting bacteria16S rRNA geneWater stress |
| spellingShingle | Kumar Shreshtha Aman Prakash Prashant Kumar Pandey Arun Kumar Pal Jyotsna Singh Pooja Tripathi Debasis Mitra Durgesh Kumar Jaiswal Sergio de los Santos-Villalobos Vijay Tripathi Isolation and characterization of plant growth promoting rhizobacteria from cacti root under drought condition Current Research in Microbial Sciences Cacti Drought Plant-associated bacteria Plant growth-promoting bacteria 16S rRNA gene Water stress |
| title | Isolation and characterization of plant growth promoting rhizobacteria from cacti root under drought condition |
| title_full | Isolation and characterization of plant growth promoting rhizobacteria from cacti root under drought condition |
| title_fullStr | Isolation and characterization of plant growth promoting rhizobacteria from cacti root under drought condition |
| title_full_unstemmed | Isolation and characterization of plant growth promoting rhizobacteria from cacti root under drought condition |
| title_short | Isolation and characterization of plant growth promoting rhizobacteria from cacti root under drought condition |
| title_sort | isolation and characterization of plant growth promoting rhizobacteria from cacti root under drought condition |
| topic | Cacti Drought Plant-associated bacteria Plant growth-promoting bacteria 16S rRNA gene Water stress |
| url | http://www.sciencedirect.com/science/article/pii/S2666517424001020 |
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