Causal relationship between gut microbiota, plasma metabolites, inflammatory cytokines and abdominal aortic aneurysm: a Mendelian randomization study

Background Complex interconnections are evident among gut microbiota, circulating metabolites, inflammatory cytokines, and the pathogenesis of abdominal aortic aneurysms (AAA), with the causal dynamics yet to be comprehensively elucidated. The primary objective of this study was to elucidate the pot...

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Main Authors: Chaozhong Li, Zhengqing Liu, Siqian Yang, Wanrong Li, Bo Liang, Haoyu Chen, Yujia Ye, Fang Wang, Xiaoqing Liu, Yongliang Jiang, Haiying Wu, Yunzhu Peng, Zhaohui Meng
Format: Article
Language:English
Published: Taylor & Francis Group 2024-12-01
Series:Clinical and Experimental Hypertension
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Online Access:https://www.tandfonline.com/doi/10.1080/10641963.2024.2390419
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author Chaozhong Li
Zhengqing Liu
Siqian Yang
Wanrong Li
Bo Liang
Haoyu Chen
Yujia Ye
Fang Wang
Xiaoqing Liu
Yongliang Jiang
Haiying Wu
Yunzhu Peng
Zhaohui Meng
author_facet Chaozhong Li
Zhengqing Liu
Siqian Yang
Wanrong Li
Bo Liang
Haoyu Chen
Yujia Ye
Fang Wang
Xiaoqing Liu
Yongliang Jiang
Haiying Wu
Yunzhu Peng
Zhaohui Meng
author_sort Chaozhong Li
collection DOAJ
description Background Complex interconnections are evident among gut microbiota, circulating metabolites, inflammatory cytokines, and the pathogenesis of abdominal aortic aneurysms (AAA), with the causal dynamics yet to be comprehensively elucidated. The primary objective of this study was to elucidate the potential causal relationships involving gut microbiota-mediated plasma metabolites, inflammatory cytokines, and AAA.Methods We utilized data from genome-wide association studies predominantly comprising individuals of European ancestry, encompassing four major gut microbiota signatures, 233 plasma metabolite signatures (N = 136,016), 91 inflammatory cytokine signatures (N = 14,824), and AAA signatures (N = 1,458,875). Mendelian randomization (MR), employed in a two-sample format, was utilized as a tool to investigate the potential causal pathways from gut microbiota to the development of AAA. Additionally, a two-step MR approach was employed to dissect the impact of plasma metabolites and inflammatory cytokines on the relationship between gut microbiota and AAA and to ascertain the mediated fractions.Results Our findings indicate that five phylum or family-identical bacteria, 175 plasma metabolites, and seven inflammatory factors are causally associated with AAA. Among them, five bacterial species from the same phylum or family, identified from different GWAS data, were strongly associated with AAA. Of these, two exhibited negative causality and three exhibited positive causality. We found that the phylum Firmicutes and the families Oscillospiraceae might reduce the risk of AAA, whereas the families Prevotellaceae, Sutterellaceae, and Aminobacteriaceae might increase the risk of AAA. Further screening indicated that phylum Firmicutes id.1672 (GCST90017114) may confer a protective effect against AAA by reducing triglyceride levels in medium/small high-density lipoprotein (HDL).Conclusion MR analysis has delineated a causal pathway from gut microbiota, through plasma circulating metabolites and inflammatory cytokines, to the pathogenesis of AAA. The role of intestinal flora and certain biomarkers may provide a reference for the diagnosis of AAA, and contribute to the prevention, diagnosis, and treatment of AAA disease.
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spelling doaj-art-a39a736e19ec473e8efee6b28528f5f02025-08-20T02:49:05ZengTaylor & Francis GroupClinical and Experimental Hypertension1064-19631525-60062024-12-0146110.1080/10641963.2024.2390419Causal relationship between gut microbiota, plasma metabolites, inflammatory cytokines and abdominal aortic aneurysm: a Mendelian randomization studyChaozhong Li0Zhengqing Liu1Siqian Yang2Wanrong Li3Bo Liang4Haoyu Chen5Yujia Ye6Fang Wang7Xiaoqing Liu8Yongliang Jiang9Haiying Wu10Yunzhu Peng11Zhaohui Meng12Department of Cardiology, The First Affiliated Hospital of Kunming Medical University, Kunming, ChinaDepartment of Cardiology, The First Affiliated Hospital of Kunming Medical University, Kunming, ChinaDepartment of Cardiology, The First Affiliated Hospital of Kunming Medical University, Kunming, ChinaDepartment of Intensive Care Unit, The People’s Hospital of Yunnan Chuxiong Yi Autonomous Prefecture, Chuxiong, ChinaDepartment of Gynecology, Sheng Ai Hospital of TCM, Kunming, ChinaDepartment of Gynecology, Sheng Ai Hospital of TCM, Kunming, ChinaDepartment of Cardiology, The First Affiliated Hospital of Kunming Medical University, Kunming, ChinaDepartment of Cardiology, The First Affiliated Hospital of Kunming Medical University, Kunming, ChinaDepartment of Cardiology, The First Affiliated Hospital of Kunming Medical University, Kunming, ChinaDepartment of Yunnan Key Laboratory of Stem Cell and Regenerative Medicine, Kunming Medical University, Kunming, ChinaDepartment of Cardiology, The First Affiliated Hospital of Kunming Medical University, Kunming, ChinaDepartment of Cardiology, The First Affiliated Hospital of Kunming Medical University, Kunming, ChinaDepartment of Cardiology, The First Affiliated Hospital of Kunming Medical University, Kunming, ChinaBackground Complex interconnections are evident among gut microbiota, circulating metabolites, inflammatory cytokines, and the pathogenesis of abdominal aortic aneurysms (AAA), with the causal dynamics yet to be comprehensively elucidated. The primary objective of this study was to elucidate the potential causal relationships involving gut microbiota-mediated plasma metabolites, inflammatory cytokines, and AAA.Methods We utilized data from genome-wide association studies predominantly comprising individuals of European ancestry, encompassing four major gut microbiota signatures, 233 plasma metabolite signatures (N = 136,016), 91 inflammatory cytokine signatures (N = 14,824), and AAA signatures (N = 1,458,875). Mendelian randomization (MR), employed in a two-sample format, was utilized as a tool to investigate the potential causal pathways from gut microbiota to the development of AAA. Additionally, a two-step MR approach was employed to dissect the impact of plasma metabolites and inflammatory cytokines on the relationship between gut microbiota and AAA and to ascertain the mediated fractions.Results Our findings indicate that five phylum or family-identical bacteria, 175 plasma metabolites, and seven inflammatory factors are causally associated with AAA. Among them, five bacterial species from the same phylum or family, identified from different GWAS data, were strongly associated with AAA. Of these, two exhibited negative causality and three exhibited positive causality. We found that the phylum Firmicutes and the families Oscillospiraceae might reduce the risk of AAA, whereas the families Prevotellaceae, Sutterellaceae, and Aminobacteriaceae might increase the risk of AAA. Further screening indicated that phylum Firmicutes id.1672 (GCST90017114) may confer a protective effect against AAA by reducing triglyceride levels in medium/small high-density lipoprotein (HDL).Conclusion MR analysis has delineated a causal pathway from gut microbiota, through plasma circulating metabolites and inflammatory cytokines, to the pathogenesis of AAA. The role of intestinal flora and certain biomarkers may provide a reference for the diagnosis of AAA, and contribute to the prevention, diagnosis, and treatment of AAA disease.https://www.tandfonline.com/doi/10.1080/10641963.2024.2390419Abdominal aortic aeurysmgut microbiotaplasma metabolitesinflammatory cytokinesMendelian randomizationmediation analyses
spellingShingle Chaozhong Li
Zhengqing Liu
Siqian Yang
Wanrong Li
Bo Liang
Haoyu Chen
Yujia Ye
Fang Wang
Xiaoqing Liu
Yongliang Jiang
Haiying Wu
Yunzhu Peng
Zhaohui Meng
Causal relationship between gut microbiota, plasma metabolites, inflammatory cytokines and abdominal aortic aneurysm: a Mendelian randomization study
Clinical and Experimental Hypertension
Abdominal aortic aeurysm
gut microbiota
plasma metabolites
inflammatory cytokines
Mendelian randomization
mediation analyses
title Causal relationship between gut microbiota, plasma metabolites, inflammatory cytokines and abdominal aortic aneurysm: a Mendelian randomization study
title_full Causal relationship between gut microbiota, plasma metabolites, inflammatory cytokines and abdominal aortic aneurysm: a Mendelian randomization study
title_fullStr Causal relationship between gut microbiota, plasma metabolites, inflammatory cytokines and abdominal aortic aneurysm: a Mendelian randomization study
title_full_unstemmed Causal relationship between gut microbiota, plasma metabolites, inflammatory cytokines and abdominal aortic aneurysm: a Mendelian randomization study
title_short Causal relationship between gut microbiota, plasma metabolites, inflammatory cytokines and abdominal aortic aneurysm: a Mendelian randomization study
title_sort causal relationship between gut microbiota plasma metabolites inflammatory cytokines and abdominal aortic aneurysm a mendelian randomization study
topic Abdominal aortic aeurysm
gut microbiota
plasma metabolites
inflammatory cytokines
Mendelian randomization
mediation analyses
url https://www.tandfonline.com/doi/10.1080/10641963.2024.2390419
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