Antimicrobial Potential of Bacteriophages JG005 and JG024 Against <i>Pseudomonas aeruginosa</i> Isolates from Canine Otitis
Canine otitis externa caused by <i>Pseudomonas aeruginosa</i> is a relevant disease in veterinary medicine. Given <i>P. aeruginosa’s</i> high priority status for the development of new antimicrobials, innovative strategies like bacteriophage therapy are essential. Lytic bacte...
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2025-07-01
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| author | Maura R. Lourenço Eva Cunha Luís Tavares Manuela Oliveira |
| author_facet | Maura R. Lourenço Eva Cunha Luís Tavares Manuela Oliveira |
| author_sort | Maura R. Lourenço |
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| description | Canine otitis externa caused by <i>Pseudomonas aeruginosa</i> is a relevant disease in veterinary medicine. Given <i>P. aeruginosa’s</i> high priority status for the development of new antimicrobials, innovative strategies like bacteriophage therapy are essential. Lytic bacteriophages are viruses with high specificity for their bacterial hosts, making them a promising therapeutic choice in both human and veterinary medicine. This study aimed to evaluate the antimicrobial potential of bacteriophages JG005 and JG024, first characterized in terms of their biofilm-forming ability and antimicrobial susceptibility profile, against <i>P. aeruginosa</i> isolates obtained from dogs with otitis externa,. Bacteriophages titer, host range, and activity were assessed against <i>P. aeruginosa</i> biofilms via microtiter assays using crystal violet and Alamar Blue. JG024 showed lytic activity against 61.2% (n = 30/49) of the isolates, while JG005 showed lytic activity against 38.8% (n = 19/49) of the isolates. Crystal violet quantification showed that JG005 can promote strong microbial suppression of 60% (n = 6/10) and 50% (n = 5/10) of the isolates at a multiplicity of infection (MOI) of 10 and 100, respectively. JG024 presented strong microbial suppression of 20% (n = 2/10) of the isolates regardless of the MOI level tested. These phages show promising potential as an innovative treatment for canine otitis externa caused by <i>P. aeruginosa</i>, but further studies are needed before future clinical use. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-07-01 |
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| spelling | doaj-art-a38e3fd486734165bdeeb3fdd9d0cdfa2025-08-20T03:32:28ZengMDPI AGVeterinary Sciences2306-73812025-07-0112764610.3390/vetsci12070646Antimicrobial Potential of Bacteriophages JG005 and JG024 Against <i>Pseudomonas aeruginosa</i> Isolates from Canine OtitisMaura R. Lourenço0Eva Cunha1Luís Tavares2Manuela Oliveira3CIISA—Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, PortugalCIISA—Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, PortugalCIISA—Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, PortugalCIISA—Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, PortugalCanine otitis externa caused by <i>Pseudomonas aeruginosa</i> is a relevant disease in veterinary medicine. Given <i>P. aeruginosa’s</i> high priority status for the development of new antimicrobials, innovative strategies like bacteriophage therapy are essential. Lytic bacteriophages are viruses with high specificity for their bacterial hosts, making them a promising therapeutic choice in both human and veterinary medicine. This study aimed to evaluate the antimicrobial potential of bacteriophages JG005 and JG024, first characterized in terms of their biofilm-forming ability and antimicrobial susceptibility profile, against <i>P. aeruginosa</i> isolates obtained from dogs with otitis externa,. Bacteriophages titer, host range, and activity were assessed against <i>P. aeruginosa</i> biofilms via microtiter assays using crystal violet and Alamar Blue. JG024 showed lytic activity against 61.2% (n = 30/49) of the isolates, while JG005 showed lytic activity against 38.8% (n = 19/49) of the isolates. Crystal violet quantification showed that JG005 can promote strong microbial suppression of 60% (n = 6/10) and 50% (n = 5/10) of the isolates at a multiplicity of infection (MOI) of 10 and 100, respectively. JG024 presented strong microbial suppression of 20% (n = 2/10) of the isolates regardless of the MOI level tested. These phages show promising potential as an innovative treatment for canine otitis externa caused by <i>P. aeruginosa</i>, but further studies are needed before future clinical use.https://www.mdpi.com/2306-7381/12/7/646antimicrobial resistancebacteriophagebiofilmdogotitis externa<i>Pseudomonas aeruginosa</i> |
| spellingShingle | Maura R. Lourenço Eva Cunha Luís Tavares Manuela Oliveira Antimicrobial Potential of Bacteriophages JG005 and JG024 Against <i>Pseudomonas aeruginosa</i> Isolates from Canine Otitis Veterinary Sciences antimicrobial resistance bacteriophage biofilm dog otitis externa <i>Pseudomonas aeruginosa</i> |
| title | Antimicrobial Potential of Bacteriophages JG005 and JG024 Against <i>Pseudomonas aeruginosa</i> Isolates from Canine Otitis |
| title_full | Antimicrobial Potential of Bacteriophages JG005 and JG024 Against <i>Pseudomonas aeruginosa</i> Isolates from Canine Otitis |
| title_fullStr | Antimicrobial Potential of Bacteriophages JG005 and JG024 Against <i>Pseudomonas aeruginosa</i> Isolates from Canine Otitis |
| title_full_unstemmed | Antimicrobial Potential of Bacteriophages JG005 and JG024 Against <i>Pseudomonas aeruginosa</i> Isolates from Canine Otitis |
| title_short | Antimicrobial Potential of Bacteriophages JG005 and JG024 Against <i>Pseudomonas aeruginosa</i> Isolates from Canine Otitis |
| title_sort | antimicrobial potential of bacteriophages jg005 and jg024 against i pseudomonas aeruginosa i isolates from canine otitis |
| topic | antimicrobial resistance bacteriophage biofilm dog otitis externa <i>Pseudomonas aeruginosa</i> |
| url | https://www.mdpi.com/2306-7381/12/7/646 |
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