Kinetic changes in microglia-related retinal transcripts in experimental autoimmune uveoretinitis (EAU) of B10.RIII mice
Abstract In this study the retinal transcriptome was investigated during the development of experimental autoimmune uveoretinitis (EAU) in mice. EAU was induced by immunizing B10.RIII mice with human interphotoreceptor retinoid binding protein (hIRBP) 161–180 peptide. Genome-wide transcriptional pro...
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BMC
2025-02-01
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| Series: | Journal of Neuroinflammation |
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| Online Access: | https://doi.org/10.1186/s12974-025-03358-x |
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| author | Maren Kasper Marcus Karlstetter Lena Wildschütz Rebecca Scholz Martin Busch Dirk Bauer Gerd Meyer zu Hörste Solon Thanos Thomas Langmann Arnd Heiligenhaus |
| author_facet | Maren Kasper Marcus Karlstetter Lena Wildschütz Rebecca Scholz Martin Busch Dirk Bauer Gerd Meyer zu Hörste Solon Thanos Thomas Langmann Arnd Heiligenhaus |
| author_sort | Maren Kasper |
| collection | DOAJ |
| description | Abstract In this study the retinal transcriptome was investigated during the development of experimental autoimmune uveoretinitis (EAU) in mice. EAU was induced by immunizing B10.RIII mice with human interphotoreceptor retinoid binding protein (hIRBP) 161–180 peptide. Genome-wide transcriptional profiles of EAU (day 7, 14 or 21 after immunization) and of control retinas were generated using DNA-microarrays and bioinformatic data mining. Microglia-associated transcripts were identified. Quantitative real-time polymerase chain reaction was performed to validate the expression of differentially expressed genes. Retinal transcript validation revealed that complement and interferon-related pathways, as well as gene clusters specific for antigen-processing and -presentation, and immunosuppression are involved during the course of the disease. Immunofluorescence analysis confirm that upregulated transcripts in EAU are also expressed by retinal microglia. Furthermore, the heterogenous expression patterns observed in retinal microglia, suggests the presence of different subpopulations of retinal microglia in EAU. This study expands our knowledge of the local immune processes involved in EAU pathology. |
| format | Article |
| id | doaj-art-a38a0cb02039415eb6dfdfaa4550fc36 |
| institution | DOAJ |
| issn | 1742-2094 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Neuroinflammation |
| spelling | doaj-art-a38a0cb02039415eb6dfdfaa4550fc362025-08-20T02:48:16ZengBMCJournal of Neuroinflammation1742-20942025-02-0122111110.1186/s12974-025-03358-xKinetic changes in microglia-related retinal transcripts in experimental autoimmune uveoretinitis (EAU) of B10.RIII miceMaren Kasper0Marcus Karlstetter1Lena Wildschütz2Rebecca Scholz3Martin Busch4Dirk Bauer5Gerd Meyer zu Hörste6Solon Thanos7Thomas Langmann8Arnd Heiligenhaus9Department of Ophthalmology at St. Franziskus Hospital, Ophtha-LabChair of Experimental Immunology of the Eye, Department of Ophthalmology, University of CologneDepartment of Ophthalmology at St. Franziskus Hospital, Ophtha-LabChair of Experimental Immunology of the Eye, Department of Ophthalmology, University of CologneDepartment of Ophthalmology at St. Franziskus Hospital, Ophtha-LabDepartment of Ophthalmology at St. Franziskus Hospital, Ophtha-LabDepartment of Neurology, University Hospital MünsterInstitute for Experimental Ophthalmology, Westfalian Wilhelms-University of MünsterChair of Experimental Immunology of the Eye, Department of Ophthalmology, University of CologneDepartment of Ophthalmology at St. Franziskus Hospital, Ophtha-LabAbstract In this study the retinal transcriptome was investigated during the development of experimental autoimmune uveoretinitis (EAU) in mice. EAU was induced by immunizing B10.RIII mice with human interphotoreceptor retinoid binding protein (hIRBP) 161–180 peptide. Genome-wide transcriptional profiles of EAU (day 7, 14 or 21 after immunization) and of control retinas were generated using DNA-microarrays and bioinformatic data mining. Microglia-associated transcripts were identified. Quantitative real-time polymerase chain reaction was performed to validate the expression of differentially expressed genes. Retinal transcript validation revealed that complement and interferon-related pathways, as well as gene clusters specific for antigen-processing and -presentation, and immunosuppression are involved during the course of the disease. Immunofluorescence analysis confirm that upregulated transcripts in EAU are also expressed by retinal microglia. Furthermore, the heterogenous expression patterns observed in retinal microglia, suggests the presence of different subpopulations of retinal microglia in EAU. This study expands our knowledge of the local immune processes involved in EAU pathology.https://doi.org/10.1186/s12974-025-03358-xRetinal transcriptomeEAUAutoimmune uveitisMicroglia |
| spellingShingle | Maren Kasper Marcus Karlstetter Lena Wildschütz Rebecca Scholz Martin Busch Dirk Bauer Gerd Meyer zu Hörste Solon Thanos Thomas Langmann Arnd Heiligenhaus Kinetic changes in microglia-related retinal transcripts in experimental autoimmune uveoretinitis (EAU) of B10.RIII mice Journal of Neuroinflammation Retinal transcriptome EAU Autoimmune uveitis Microglia |
| title | Kinetic changes in microglia-related retinal transcripts in experimental autoimmune uveoretinitis (EAU) of B10.RIII mice |
| title_full | Kinetic changes in microglia-related retinal transcripts in experimental autoimmune uveoretinitis (EAU) of B10.RIII mice |
| title_fullStr | Kinetic changes in microglia-related retinal transcripts in experimental autoimmune uveoretinitis (EAU) of B10.RIII mice |
| title_full_unstemmed | Kinetic changes in microglia-related retinal transcripts in experimental autoimmune uveoretinitis (EAU) of B10.RIII mice |
| title_short | Kinetic changes in microglia-related retinal transcripts in experimental autoimmune uveoretinitis (EAU) of B10.RIII mice |
| title_sort | kinetic changes in microglia related retinal transcripts in experimental autoimmune uveoretinitis eau of b10 riii mice |
| topic | Retinal transcriptome EAU Autoimmune uveitis Microglia |
| url | https://doi.org/10.1186/s12974-025-03358-x |
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