SARS-CoV-2 survival on skin and its transfer from contaminated surfaces.

Understanding the transmission dynamics of SARS-CoV-2, particularly its transfer from contaminated surfaces (fomites) to human skin, is crucial for mitigating the spread of COVID-19. While extensive research has examined the persistence of SARS-CoV-2 on various surfaces, there is limited understandi...

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Main Authors: Ana K Pitol, Samiksha Venkatesan, Siobhan Richards, Michael Hoptroff, Amitabha Majumdar, Grant L Hughes
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2025-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0325235
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author Ana K Pitol
Samiksha Venkatesan
Siobhan Richards
Michael Hoptroff
Amitabha Majumdar
Grant L Hughes
author_facet Ana K Pitol
Samiksha Venkatesan
Siobhan Richards
Michael Hoptroff
Amitabha Majumdar
Grant L Hughes
author_sort Ana K Pitol
collection DOAJ
description Understanding the transmission dynamics of SARS-CoV-2, particularly its transfer from contaminated surfaces (fomites) to human skin, is crucial for mitigating the spread of COVID-19. While extensive research has examined the persistence of SARS-CoV-2 on various surfaces, there is limited understanding of how efficiently it transfers to human skin, and how long it survives on the skin. This study investigates two key aspects of SARS-CoV-2 transmission: (1) the transfer efficiency of SARS-CoV-2 from non-porous (plastic and metal) and porous (cardboard) surfaces to a 3D human skin model (LabSkin), and (2) the survival of SARS-CoV-2 on the skin under different temperature conditions. First, we validated LabSkin as a suitable surrogate for human skin by comparing the transfer efficiency of the bacteriophage Phi 6 from surfaces to LabSkin and to human volunteers' fingers. No significant differences were observed, confirming LabSkin's suitability for these studies. Subsequently, the transfer of SARS-CoV-2 from surfaces to LabSkin was assessed, showing that plastic and metal surfaces had similar transfer efficiencies (~13%), while no transfer occurred from cardboard once the inoculum had dried on the surface. Finally, the survival of SARS-CoV-2 on skin was assessed, showing a rapid decay at higher temperatures, with a half-life ranging from 2.8 to 17.8 hours depending on the temperature. These findings enhance our understanding of viral transmission via fomites and inform public health strategies to reduce the risk of SARS-CoV-2 transmission through surface contact.
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spelling doaj-art-a374eacec23c40d9be9784393ac0939d2025-08-20T03:26:29ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01206e032523510.1371/journal.pone.0325235SARS-CoV-2 survival on skin and its transfer from contaminated surfaces.Ana K PitolSamiksha VenkatesanSiobhan RichardsMichael HoptroffAmitabha MajumdarGrant L HughesUnderstanding the transmission dynamics of SARS-CoV-2, particularly its transfer from contaminated surfaces (fomites) to human skin, is crucial for mitigating the spread of COVID-19. While extensive research has examined the persistence of SARS-CoV-2 on various surfaces, there is limited understanding of how efficiently it transfers to human skin, and how long it survives on the skin. This study investigates two key aspects of SARS-CoV-2 transmission: (1) the transfer efficiency of SARS-CoV-2 from non-porous (plastic and metal) and porous (cardboard) surfaces to a 3D human skin model (LabSkin), and (2) the survival of SARS-CoV-2 on the skin under different temperature conditions. First, we validated LabSkin as a suitable surrogate for human skin by comparing the transfer efficiency of the bacteriophage Phi 6 from surfaces to LabSkin and to human volunteers' fingers. No significant differences were observed, confirming LabSkin's suitability for these studies. Subsequently, the transfer of SARS-CoV-2 from surfaces to LabSkin was assessed, showing that plastic and metal surfaces had similar transfer efficiencies (~13%), while no transfer occurred from cardboard once the inoculum had dried on the surface. Finally, the survival of SARS-CoV-2 on skin was assessed, showing a rapid decay at higher temperatures, with a half-life ranging from 2.8 to 17.8 hours depending on the temperature. These findings enhance our understanding of viral transmission via fomites and inform public health strategies to reduce the risk of SARS-CoV-2 transmission through surface contact.https://doi.org/10.1371/journal.pone.0325235
spellingShingle Ana K Pitol
Samiksha Venkatesan
Siobhan Richards
Michael Hoptroff
Amitabha Majumdar
Grant L Hughes
SARS-CoV-2 survival on skin and its transfer from contaminated surfaces.
PLoS ONE
title SARS-CoV-2 survival on skin and its transfer from contaminated surfaces.
title_full SARS-CoV-2 survival on skin and its transfer from contaminated surfaces.
title_fullStr SARS-CoV-2 survival on skin and its transfer from contaminated surfaces.
title_full_unstemmed SARS-CoV-2 survival on skin and its transfer from contaminated surfaces.
title_short SARS-CoV-2 survival on skin and its transfer from contaminated surfaces.
title_sort sars cov 2 survival on skin and its transfer from contaminated surfaces
url https://doi.org/10.1371/journal.pone.0325235
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