Arsenic Species Analysis at Trace Level by High Performance Liquid Chromatography with Inductively Coupled Plasma Mass Spectrometry

A sensitive and accurate simultaneous continuous analysis for six arsenic species including arsenobetaine (AsB), arsenocholine (AsC), monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), arsenite (AsIII), and arsenate (AsV) has been developed by high performance liquid chromatography with induc...

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Main Authors: Hongfang Hou, Wanjing Cui, Qing Xu, Zhanhui Tao, Yafei Guo, Tianlong Deng
Format: Article
Language:English
Published: Wiley 2019-01-01
Series:International Journal of Analytical Chemistry
Online Access:http://dx.doi.org/10.1155/2019/3280840
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author Hongfang Hou
Wanjing Cui
Qing Xu
Zhanhui Tao
Yafei Guo
Tianlong Deng
author_facet Hongfang Hou
Wanjing Cui
Qing Xu
Zhanhui Tao
Yafei Guo
Tianlong Deng
author_sort Hongfang Hou
collection DOAJ
description A sensitive and accurate simultaneous continuous analysis for six arsenic species including arsenobetaine (AsB), arsenocholine (AsC), monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), arsenite (AsIII), and arsenate (AsV) has been developed by high performance liquid chromatography with inductively coupled plasma mass spectrometry (HPLC-ICP-MS). An anion-exchange column of Hamilton PRP-X100 (Switzerland) was applied for separation of the six arsenic species with gradient elution of 1.25 mmol/L Na2HPO4 and 11.0 mmol/L KH2PO4 as the mobile phase A and 2.5 mmol/L Na2HPO4 and 22.0 mmol/L KH2PO4 as the mobile phase B. The linearity ranges for AsB, AsC, MMA, DMA, AsIII, and AsV were between 0.5 and 50.0 μg/L, and the detection limits of the six arsenic species were all within 0.01–0.35 ng/L. The relative standard deviations (RSDs) were within 2.26–3.68% and the recovery rates of samples ranged from 95 to 103%. The proposed method was applied for the arsenic speciation analysis of sediment pore-water samples, which were taken from the supernatant after centrifugation and filtration.
format Article
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institution Kabale University
issn 1687-8760
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language English
publishDate 2019-01-01
publisher Wiley
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series International Journal of Analytical Chemistry
spelling doaj-art-a367b188a04448a69848d488a2b69bdb2025-08-20T03:38:18ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792019-01-01201910.1155/2019/32808403280840Arsenic Species Analysis at Trace Level by High Performance Liquid Chromatography with Inductively Coupled Plasma Mass SpectrometryHongfang Hou0Wanjing Cui1Qing Xu2Zhanhui Tao3Yafei Guo4Tianlong Deng5Tianjin Key Laboratory of Marine Resources and Chemistry, Modern Analytical Technology Research Center, College of Chemical Engineering and Materials Science, Tianjin University of Science and Technology, Tianjin 300457, ChinaTianjin Key Laboratory of Marine Resources and Chemistry, Modern Analytical Technology Research Center, College of Chemical Engineering and Materials Science, Tianjin University of Science and Technology, Tianjin 300457, ChinaTianjin Key Laboratory of Marine Resources and Chemistry, Modern Analytical Technology Research Center, College of Chemical Engineering and Materials Science, Tianjin University of Science and Technology, Tianjin 300457, ChinaTianjin Key Laboratory of Marine Resources and Chemistry, Modern Analytical Technology Research Center, College of Chemical Engineering and Materials Science, Tianjin University of Science and Technology, Tianjin 300457, ChinaTianjin Key Laboratory of Marine Resources and Chemistry, Modern Analytical Technology Research Center, College of Chemical Engineering and Materials Science, Tianjin University of Science and Technology, Tianjin 300457, ChinaTianjin Key Laboratory of Marine Resources and Chemistry, Modern Analytical Technology Research Center, College of Chemical Engineering and Materials Science, Tianjin University of Science and Technology, Tianjin 300457, ChinaA sensitive and accurate simultaneous continuous analysis for six arsenic species including arsenobetaine (AsB), arsenocholine (AsC), monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), arsenite (AsIII), and arsenate (AsV) has been developed by high performance liquid chromatography with inductively coupled plasma mass spectrometry (HPLC-ICP-MS). An anion-exchange column of Hamilton PRP-X100 (Switzerland) was applied for separation of the six arsenic species with gradient elution of 1.25 mmol/L Na2HPO4 and 11.0 mmol/L KH2PO4 as the mobile phase A and 2.5 mmol/L Na2HPO4 and 22.0 mmol/L KH2PO4 as the mobile phase B. The linearity ranges for AsB, AsC, MMA, DMA, AsIII, and AsV were between 0.5 and 50.0 μg/L, and the detection limits of the six arsenic species were all within 0.01–0.35 ng/L. The relative standard deviations (RSDs) were within 2.26–3.68% and the recovery rates of samples ranged from 95 to 103%. The proposed method was applied for the arsenic speciation analysis of sediment pore-water samples, which were taken from the supernatant after centrifugation and filtration.http://dx.doi.org/10.1155/2019/3280840
spellingShingle Hongfang Hou
Wanjing Cui
Qing Xu
Zhanhui Tao
Yafei Guo
Tianlong Deng
Arsenic Species Analysis at Trace Level by High Performance Liquid Chromatography with Inductively Coupled Plasma Mass Spectrometry
International Journal of Analytical Chemistry
title Arsenic Species Analysis at Trace Level by High Performance Liquid Chromatography with Inductively Coupled Plasma Mass Spectrometry
title_full Arsenic Species Analysis at Trace Level by High Performance Liquid Chromatography with Inductively Coupled Plasma Mass Spectrometry
title_fullStr Arsenic Species Analysis at Trace Level by High Performance Liquid Chromatography with Inductively Coupled Plasma Mass Spectrometry
title_full_unstemmed Arsenic Species Analysis at Trace Level by High Performance Liquid Chromatography with Inductively Coupled Plasma Mass Spectrometry
title_short Arsenic Species Analysis at Trace Level by High Performance Liquid Chromatography with Inductively Coupled Plasma Mass Spectrometry
title_sort arsenic species analysis at trace level by high performance liquid chromatography with inductively coupled plasma mass spectrometry
url http://dx.doi.org/10.1155/2019/3280840
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AT wanjingcui arsenicspeciesanalysisattracelevelbyhighperformanceliquidchromatographywithinductivelycoupledplasmamassspectrometry
AT qingxu arsenicspeciesanalysisattracelevelbyhighperformanceliquidchromatographywithinductivelycoupledplasmamassspectrometry
AT zhanhuitao arsenicspeciesanalysisattracelevelbyhighperformanceliquidchromatographywithinductivelycoupledplasmamassspectrometry
AT yafeiguo arsenicspeciesanalysisattracelevelbyhighperformanceliquidchromatographywithinductivelycoupledplasmamassspectrometry
AT tianlongdeng arsenicspeciesanalysisattracelevelbyhighperformanceliquidchromatographywithinductivelycoupledplasmamassspectrometry