Evaluation the Surface Antigen of the Salmonella typhimurium ATCC 14028 Ghosts Prepared by “SLRP”
Recently, bacterial ghosts (BGs) were prepared using a protocol based on critical chemical concentrations. It has been given the name “sponge like” (SL) protocol and used in its reduced form “sponge like reduced protocol” (SLRP). While specific antibody for Salmonella is available on the market und...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/840863 |
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| author | Amara A. Amro Ahmed J. Neama Ahmed Hussein Emad A. Hashish Salah A. Sheweita |
| author_facet | Amara A. Amro Ahmed J. Neama Ahmed Hussein Emad A. Hashish Salah A. Sheweita |
| author_sort | Amara A. Amro |
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| description | Recently, bacterial ghosts (BGs) were prepared using a protocol based on critical chemical concentrations. It has been given the name “sponge like” (SL) protocol and used in its reduced form “sponge like reduced protocol” (SLRP). While specific antibody for Salmonella is available on the market under the commercial names (of some kits) such as Febrile Antigen Kit (N.S. BIO-TEC), we used the described Kit to investigate the validity of the SLRP. In this study, using SLRP we succeeded to prepare STGs with correct surface antigens could interact with their specific antibodies. Additionally the study has included oral vaccination with STGs with challenge test. The rats serums have been evaluated against both of the O and H antigens. The antigen-antibody interaction (agglutination) results of both the SLRP and the animal experiments prove that we have correct STGs able to immunize the rats against viable Salmonella. STGs could be used as vaccine and as adjuvant and in the antibodies and in the diagnostic kits production. This study is an additional step for the establishment of correct BGs for immunological purposes. |
| format | Article |
| id | doaj-art-a3653d49ac264e8a921094e43abd4169 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-a3653d49ac264e8a921094e43abd41692025-08-20T03:38:18ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/840863840863Evaluation the Surface Antigen of the Salmonella typhimurium ATCC 14028 Ghosts Prepared by “SLRP”Amara A. Amro0Ahmed J. Neama1Ahmed Hussein2Emad A. Hashish3Salah A. Sheweita4Department of Protein Research, Genetic Engineering and Biotechnology Research Institute, City for Scientific Research and Technology Applications, New Borg Al-Arab, P.O. Box. 21934, Alexandria, EgyptZoonoses Research Unit, Faculty of Veterinary Medicine, Alqadisya University, Alqadisya, IraqBiotechnology Department, Institute of Graduate Studies & Research, Alexandria University, 163 El Horreya Avenu, P.O. Box 832, Alexandria 21526, EgyptDepartment of Clinical Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, EgyptBiotechnology Department, Institute of Graduate Studies & Research, Alexandria University, 163 El Horreya Avenu, P.O. Box 832, Alexandria 21526, EgyptRecently, bacterial ghosts (BGs) were prepared using a protocol based on critical chemical concentrations. It has been given the name “sponge like” (SL) protocol and used in its reduced form “sponge like reduced protocol” (SLRP). While specific antibody for Salmonella is available on the market under the commercial names (of some kits) such as Febrile Antigen Kit (N.S. BIO-TEC), we used the described Kit to investigate the validity of the SLRP. In this study, using SLRP we succeeded to prepare STGs with correct surface antigens could interact with their specific antibodies. Additionally the study has included oral vaccination with STGs with challenge test. The rats serums have been evaluated against both of the O and H antigens. The antigen-antibody interaction (agglutination) results of both the SLRP and the animal experiments prove that we have correct STGs able to immunize the rats against viable Salmonella. STGs could be used as vaccine and as adjuvant and in the antibodies and in the diagnostic kits production. This study is an additional step for the establishment of correct BGs for immunological purposes.http://dx.doi.org/10.1155/2014/840863 |
| spellingShingle | Amara A. Amro Ahmed J. Neama Ahmed Hussein Emad A. Hashish Salah A. Sheweita Evaluation the Surface Antigen of the Salmonella typhimurium ATCC 14028 Ghosts Prepared by “SLRP” The Scientific World Journal |
| title | Evaluation the Surface Antigen of the Salmonella typhimurium ATCC 14028 Ghosts Prepared by “SLRP” |
| title_full | Evaluation the Surface Antigen of the Salmonella typhimurium ATCC 14028 Ghosts Prepared by “SLRP” |
| title_fullStr | Evaluation the Surface Antigen of the Salmonella typhimurium ATCC 14028 Ghosts Prepared by “SLRP” |
| title_full_unstemmed | Evaluation the Surface Antigen of the Salmonella typhimurium ATCC 14028 Ghosts Prepared by “SLRP” |
| title_short | Evaluation the Surface Antigen of the Salmonella typhimurium ATCC 14028 Ghosts Prepared by “SLRP” |
| title_sort | evaluation the surface antigen of the salmonella typhimurium atcc 14028 ghosts prepared by slrp |
| url | http://dx.doi.org/10.1155/2014/840863 |
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