Monitoring proliferation and material production of Cupriavidus necator H16 using cell count and volume measurement
Abstract Knallgas bacteria, including Cupriavidus necator H16, are promising cell factories for converting CO2 into high-value compounds under autotrophic conditions. C. necator H16 synthesizes polyhydroxyalkanoates (PHA), a class of biodegradable plastics. However, the trade-off between cell prolif...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-08-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-16567-4 |
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| _version_ | 1849226440642396160 |
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| author | Kouhei Kamasaka Naoki Abekawa Koji Takeda Kentaro Noi Mami Matsuda Keiji Matsumoto Noboru Yumoto Akihiko Kondo Tomohisa Hasunuma |
| author_facet | Kouhei Kamasaka Naoki Abekawa Koji Takeda Kentaro Noi Mami Matsuda Keiji Matsumoto Noboru Yumoto Akihiko Kondo Tomohisa Hasunuma |
| author_sort | Kouhei Kamasaka |
| collection | DOAJ |
| description | Abstract Knallgas bacteria, including Cupriavidus necator H16, are promising cell factories for converting CO2 into high-value compounds under autotrophic conditions. C. necator H16 synthesizes polyhydroxyalkanoates (PHA), a class of biodegradable plastics. However, the trade-off between cell proliferation and PHA production often limits productivity as a result of competition for cellular resources. Real-time monitoring of both processes is crucial for optimizing this balance. However, optical density (OD), a conventional metric for monitoring proliferation, is unreliable in organisms that accumulate intracellular products such as PHA. Traditional methods such as chromatography require complex sample preparation and are not suitable for real-time analysis. This study demonstrated that the cell counts and volume measured using a Coulter counter are reliable indicators of proliferation and PHA production. This approach enables rapid and accurate monitoring and supports the optimization of material production through microbial fermentation. |
| format | Article |
| id | doaj-art-a363b4188f054fc389cf01b73aa7dbb5 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-a363b4188f054fc389cf01b73aa7dbb52025-08-24T11:21:53ZengNature PortfolioScientific Reports2045-23222025-08-0115111010.1038/s41598-025-16567-4Monitoring proliferation and material production of Cupriavidus necator H16 using cell count and volume measurementKouhei Kamasaka0Naoki Abekawa1Koji Takeda2Kentaro Noi3Mami Matsuda4Keiji Matsumoto5Noboru Yumoto6Akihiko Kondo7Tomohisa Hasunuma8Engineering Biology Research Center, Kobe UniversityGraduate School of Science, Technology and Innovation, Kobe UniversityEngineering Biology Research Center, Kobe UniversityEngineering Biology Research Center, Kobe UniversityEngineering Biology Research Center, Kobe UniversityEngineering Biology Research Center, Kobe UniversityEngineering Biology Research Center, Kobe UniversityEngineering Biology Research Center, Kobe UniversityEngineering Biology Research Center, Kobe UniversityAbstract Knallgas bacteria, including Cupriavidus necator H16, are promising cell factories for converting CO2 into high-value compounds under autotrophic conditions. C. necator H16 synthesizes polyhydroxyalkanoates (PHA), a class of biodegradable plastics. However, the trade-off between cell proliferation and PHA production often limits productivity as a result of competition for cellular resources. Real-time monitoring of both processes is crucial for optimizing this balance. However, optical density (OD), a conventional metric for monitoring proliferation, is unreliable in organisms that accumulate intracellular products such as PHA. Traditional methods such as chromatography require complex sample preparation and are not suitable for real-time analysis. This study demonstrated that the cell counts and volume measured using a Coulter counter are reliable indicators of proliferation and PHA production. This approach enables rapid and accurate monitoring and supports the optimization of material production through microbial fermentation.https://doi.org/10.1038/s41598-025-16567-4Cupriavidus necatorPolyhydroxyalkanoateCell volumeProliferationOptical density |
| spellingShingle | Kouhei Kamasaka Naoki Abekawa Koji Takeda Kentaro Noi Mami Matsuda Keiji Matsumoto Noboru Yumoto Akihiko Kondo Tomohisa Hasunuma Monitoring proliferation and material production of Cupriavidus necator H16 using cell count and volume measurement Scientific Reports Cupriavidus necator Polyhydroxyalkanoate Cell volume Proliferation Optical density |
| title | Monitoring proliferation and material production of Cupriavidus necator H16 using cell count and volume measurement |
| title_full | Monitoring proliferation and material production of Cupriavidus necator H16 using cell count and volume measurement |
| title_fullStr | Monitoring proliferation and material production of Cupriavidus necator H16 using cell count and volume measurement |
| title_full_unstemmed | Monitoring proliferation and material production of Cupriavidus necator H16 using cell count and volume measurement |
| title_short | Monitoring proliferation and material production of Cupriavidus necator H16 using cell count and volume measurement |
| title_sort | monitoring proliferation and material production of cupriavidus necator h16 using cell count and volume measurement |
| topic | Cupriavidus necator Polyhydroxyalkanoate Cell volume Proliferation Optical density |
| url | https://doi.org/10.1038/s41598-025-16567-4 |
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