Characterization of fermentation liquid from mangrove leaves Avicennia marina and its inhibitory potential for bacterium causing ice-ice disease
ABSTRACT Fermentation liquid from mangrove leaves Avicennia marina contains microorganisms, nutrients, and secondary metabolites. This study aimed to identify bacteria and the compounds in fermentation liquid of mangrove leaves A. marina and measured their inhibitory capacity against pathog...
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| Format: | Article |
| Language: | English |
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Asosiasi Sains Akuakultur Indonesia
2020-01-01
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| Series: | Jurnal Akuakultur Indonesia |
| Online Access: | https://journal.ipb.ac.id/index.php/jai/article/view/29256 |
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| author | Samsu Adi Rahman Sukenda Widanarni Alimuddin Julie Ekasari |
| author_facet | Samsu Adi Rahman Sukenda Widanarni Alimuddin Julie Ekasari |
| author_sort | Samsu Adi Rahman |
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ABSTRACT
Fermentation liquid from mangrove leaves Avicennia marina contains microorganisms, nutrients, and secondary metabolites. This study aimed to identify bacteria and the compounds in fermentation liquid of mangrove leaves A. marina and measured their inhibitory capacity against pathogenic bacteria Stenotrophomonas maltophilia which causes ice-ice disease in seaweed. Molecular analysis which aimed the 16S rRNA gene showed that the bacteria in fermentation liquid consisted of eight types of Bacillus, Bacillus subtilis MSAR-01, Bacillus megaterium MSAR-02, Bacillus firmus MSAR-03, Bacillus thuringiensis MSAR-04, Bacillus subterranerus MSAR-05, Bacillus vietnamensis MSAR-06, Bacillus sp. MSAR-07, Bacillus circulans MSAR-08, with the best inhibitory power indicated by B. subtilis MSAR-01, B. vietnamensis MSAR-06, and Bacillus sp. MSAR-07. The administration of lactic acid, bacteriocin, total fermentation liquid, and supernatant as much as 15 mL produce inhibition to S. maltophilia indicated better result than using one or a combination of several types of bacterial isolates. The inhibition of single bacterial enriched fermentation and supernatant liquids was better than bacterial combination enrichment.
Keywords: Avicennia marina, fermentation, ice-ice, mangrove
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| format | Article |
| id | doaj-art-a358fbf8f6d944089668495d1f138f20 |
| institution | Kabale University |
| issn | 1412-5269 2354-6700 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Asosiasi Sains Akuakultur Indonesia |
| record_format | Article |
| series | Jurnal Akuakultur Indonesia |
| spelling | doaj-art-a358fbf8f6d944089668495d1f138f202025-01-24T17:56:56ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002020-01-0119110.19027/jai.19.1.1-929256Characterization of fermentation liquid from mangrove leaves Avicennia marina and its inhibitory potential for bacterium causing ice-ice diseaseSamsu Adi Rahman0Sukenda1Widanarni2Alimuddin3Julie Ekasari4Study Program of Aquaculture, Faculty of Fisheries, University of Muhammadiyah Luwuk, Luwuk, IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Bogor, IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Bogor, IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Bogor, IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Bogor, Indonesia ABSTRACT Fermentation liquid from mangrove leaves Avicennia marina contains microorganisms, nutrients, and secondary metabolites. This study aimed to identify bacteria and the compounds in fermentation liquid of mangrove leaves A. marina and measured their inhibitory capacity against pathogenic bacteria Stenotrophomonas maltophilia which causes ice-ice disease in seaweed. Molecular analysis which aimed the 16S rRNA gene showed that the bacteria in fermentation liquid consisted of eight types of Bacillus, Bacillus subtilis MSAR-01, Bacillus megaterium MSAR-02, Bacillus firmus MSAR-03, Bacillus thuringiensis MSAR-04, Bacillus subterranerus MSAR-05, Bacillus vietnamensis MSAR-06, Bacillus sp. MSAR-07, Bacillus circulans MSAR-08, with the best inhibitory power indicated by B. subtilis MSAR-01, B. vietnamensis MSAR-06, and Bacillus sp. MSAR-07. The administration of lactic acid, bacteriocin, total fermentation liquid, and supernatant as much as 15 mL produce inhibition to S. maltophilia indicated better result than using one or a combination of several types of bacterial isolates. The inhibition of single bacterial enriched fermentation and supernatant liquids was better than bacterial combination enrichment. Keywords: Avicennia marina, fermentation, ice-ice, mangrove https://journal.ipb.ac.id/index.php/jai/article/view/29256 |
| spellingShingle | Samsu Adi Rahman Sukenda Widanarni Alimuddin Julie Ekasari Characterization of fermentation liquid from mangrove leaves Avicennia marina and its inhibitory potential for bacterium causing ice-ice disease Jurnal Akuakultur Indonesia |
| title | Characterization of fermentation liquid from mangrove leaves Avicennia marina and its inhibitory potential for bacterium causing ice-ice disease |
| title_full | Characterization of fermentation liquid from mangrove leaves Avicennia marina and its inhibitory potential for bacterium causing ice-ice disease |
| title_fullStr | Characterization of fermentation liquid from mangrove leaves Avicennia marina and its inhibitory potential for bacterium causing ice-ice disease |
| title_full_unstemmed | Characterization of fermentation liquid from mangrove leaves Avicennia marina and its inhibitory potential for bacterium causing ice-ice disease |
| title_short | Characterization of fermentation liquid from mangrove leaves Avicennia marina and its inhibitory potential for bacterium causing ice-ice disease |
| title_sort | characterization of fermentation liquid from mangrove leaves avicennia marina and its inhibitory potential for bacterium causing ice ice disease |
| url | https://journal.ipb.ac.id/index.php/jai/article/view/29256 |
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