Metabolomic profiling of ZrO2 nanoparticles in MC3T3‐E1 cells
Abstract The authors' previous study showed that zirconium oxide nanoparticles (ZrO2 NPs) induce toxic effects in MC3T3‐E1 cells; however, its toxicological mechanism is still unclear. Liquid chromatography–mass spectrometry/time‐of‐flight mass spectrometry was used to reveal the metabolite pro...
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| Format: | Article |
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Wiley
2021-12-01
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| Series: | IET Nanobiotechnology |
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| Online Access: | https://doi.org/10.1049/nbt2.12067 |
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| author | Mingfu Ye Linhu Wang Zhang Wu Wenjun Liu |
| author_facet | Mingfu Ye Linhu Wang Zhang Wu Wenjun Liu |
| author_sort | Mingfu Ye |
| collection | DOAJ |
| description | Abstract The authors' previous study showed that zirconium oxide nanoparticles (ZrO2 NPs) induce toxic effects in MC3T3‐E1 cells; however, its toxicological mechanism is still unclear. Liquid chromatography–mass spectrometry/time‐of‐flight mass spectrometry was used to reveal the metabolite profile and toxicological mechanism of MC3T3‐E1 cells in response to ZrO2 NPs. The results demonstrated that MC3T3‐E1 cells treated with ZrO2 NPs for 24 and 48 h presented different metabolic characteristics. Following ZrO2 NP treatment for 24 h, 96 upregulated and 129 downregulated metabolites in the positive ion mode, as well as 91 upregulated and 326 downregulated metabolites in the negative ion mode were identified. Following ZrO2 NP treatment for 48 h, 33 upregulated and 174 downregulated metabolites were identified in the positive ion mode, whereas 37 upregulated and 302 downregulated metabolites were confirmed in the negative ion mode. Among them, 42 differential metabolites were recognised as potential metabolites contributing to the induced toxic effects of ZrO2 NPs in MC3T3‐E1 cells. Most of the differential metabolites were lysophosphatidylcholine and lysophosphatidylethanolamide, indicating that exposure to ZrO2 NPs may have a profound impact on human cellular function by impairing the membrane system. The results also provide new clues for the toxicological mechanism of ZrO2 NP dental materials. |
| format | Article |
| id | doaj-art-a3464963c3874ea8afef8f248ce165e2 |
| institution | Kabale University |
| issn | 1751-8741 1751-875X |
| language | English |
| publishDate | 2021-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | IET Nanobiotechnology |
| spelling | doaj-art-a3464963c3874ea8afef8f248ce165e22025-08-20T03:38:16ZengWileyIET Nanobiotechnology1751-87411751-875X2021-12-0115968769710.1049/nbt2.12067Metabolomic profiling of ZrO2 nanoparticles in MC3T3‐E1 cellsMingfu Ye0Linhu Wang1Zhang Wu2Wenjun Liu3Department of Oral Implantology Stomatological Hospital of Xiamen Medical College Xiamen Key Laboratory of Stomatological Disease Diagnosis and Treatment Xiamen ChinaDepartment of Stomatology General Hospital of Central Theater Command Wuhan ChinaDepartment of Prosthodontics Stomatological Hospital of Xiamen Medical College Xiamen Key Laboratory of Stomatological Disease Diagnosis and Treatment Xiamen ChinaDepartment of Oral Implantology Stomatological Hospital of Xiamen Medical College Xiamen Key Laboratory of Stomatological Disease Diagnosis and Treatment Xiamen ChinaAbstract The authors' previous study showed that zirconium oxide nanoparticles (ZrO2 NPs) induce toxic effects in MC3T3‐E1 cells; however, its toxicological mechanism is still unclear. Liquid chromatography–mass spectrometry/time‐of‐flight mass spectrometry was used to reveal the metabolite profile and toxicological mechanism of MC3T3‐E1 cells in response to ZrO2 NPs. The results demonstrated that MC3T3‐E1 cells treated with ZrO2 NPs for 24 and 48 h presented different metabolic characteristics. Following ZrO2 NP treatment for 24 h, 96 upregulated and 129 downregulated metabolites in the positive ion mode, as well as 91 upregulated and 326 downregulated metabolites in the negative ion mode were identified. Following ZrO2 NP treatment for 48 h, 33 upregulated and 174 downregulated metabolites were identified in the positive ion mode, whereas 37 upregulated and 302 downregulated metabolites were confirmed in the negative ion mode. Among them, 42 differential metabolites were recognised as potential metabolites contributing to the induced toxic effects of ZrO2 NPs in MC3T3‐E1 cells. Most of the differential metabolites were lysophosphatidylcholine and lysophosphatidylethanolamide, indicating that exposure to ZrO2 NPs may have a profound impact on human cellular function by impairing the membrane system. The results also provide new clues for the toxicological mechanism of ZrO2 NP dental materials.https://doi.org/10.1049/nbt2.12067molecular biophysicsbiochemistrytoxicologybiomedical materialschromatographycellular biophysics |
| spellingShingle | Mingfu Ye Linhu Wang Zhang Wu Wenjun Liu Metabolomic profiling of ZrO2 nanoparticles in MC3T3‐E1 cells IET Nanobiotechnology molecular biophysics biochemistry toxicology biomedical materials chromatography cellular biophysics |
| title | Metabolomic profiling of ZrO2 nanoparticles in MC3T3‐E1 cells |
| title_full | Metabolomic profiling of ZrO2 nanoparticles in MC3T3‐E1 cells |
| title_fullStr | Metabolomic profiling of ZrO2 nanoparticles in MC3T3‐E1 cells |
| title_full_unstemmed | Metabolomic profiling of ZrO2 nanoparticles in MC3T3‐E1 cells |
| title_short | Metabolomic profiling of ZrO2 nanoparticles in MC3T3‐E1 cells |
| title_sort | metabolomic profiling of zro2 nanoparticles in mc3t3 e1 cells |
| topic | molecular biophysics biochemistry toxicology biomedical materials chromatography cellular biophysics |
| url | https://doi.org/10.1049/nbt2.12067 |
| work_keys_str_mv | AT mingfuye metabolomicprofilingofzro2nanoparticlesinmc3t3e1cells AT linhuwang metabolomicprofilingofzro2nanoparticlesinmc3t3e1cells AT zhangwu metabolomicprofilingofzro2nanoparticlesinmc3t3e1cells AT wenjunliu metabolomicprofilingofzro2nanoparticlesinmc3t3e1cells |