Biofilm formation by clinically isolated Staphylococcus Aureus from India
Introduction: Staphylococcal biofilms are prominent cause for acute and chronic infection both in hospital and community settings across the world. Current study explores biofilm formation by Staphylococcus aureus isolates from clinical samples by different methods. Methodology: Standard techniq...
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The Journal of Infection in Developing Countries
2018-12-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/10671 |
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| author | Alasthimannahalli Gangadhara Triveni Mendem Suresh Kumar Chavadi Manjunath Channappa T Shivannavar Subhaschandra M Gaddad |
| author_facet | Alasthimannahalli Gangadhara Triveni Mendem Suresh Kumar Chavadi Manjunath Channappa T Shivannavar Subhaschandra M Gaddad |
| author_sort | Alasthimannahalli Gangadhara Triveni |
| collection | DOAJ |
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Introduction: Staphylococcal biofilms are prominent cause for acute and chronic infection both in hospital and community settings across the world. Current study explores biofilm formation by Staphylococcus aureus isolates from clinical samples by different methods.
Methodology: Standard techniques used for the characterization of S.aureus. Qualitative and quantitative biofilm formation was assessed by Congo red Agar, Tube and Microtiter plate methods.
Results: A total of 188 clinical isolates of S.aureus were screened for biofilm formation and 72 (38.29%) of them were found to be biofilm producers, 34 (18.08%) strong, 38 (20.21%) moderate. The remaining 116 (61.7%) were weak/ non biofilm producers. Maximum biofilm formers were recorded in pus samples (39.06%), followed by isolates from blood (38.23%) and urine (34.61%). Statistical analysis for the formation of biofilm indicated that Microtiter plate method is the most sensitive and specific method for screening biofilm production.
Conclusions: Biofilm formation is one of the influential virulence factor in staphylococcal pathogenesis and persistence. Microtiter plate and Congo red agar remain as reliable methods for the qualitative and quantitative estimation of biofilm formation. Monitoring of biofilm formation in various etiological agents will help in determining the severity of infection.
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| format | Article |
| id | doaj-art-a3321ae0fbae4704be26dacbdf3b9d00 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2018-12-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-a3321ae0fbae4704be26dacbdf3b9d002025-08-20T03:48:47ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802018-12-01121210.3855/jidc.10671Biofilm formation by clinically isolated Staphylococcus Aureus from IndiaAlasthimannahalli Gangadhara Triveni0Mendem Suresh Kumar1Chavadi Manjunath2Channappa T Shivannavar3Subhaschandra M Gaddad4Gulbarga University, Kalaburagi-585106, Karnataka, IndiaGulbarga University, Kalaburagi-585106, Karnataka, IndiaGulbarga University, Kalaburagi-585106, Karnataka, IndiaGulbarga University, Kalaburagi-585106, Karnataka, IndiaGulbarga University, Kalaburagi-585106, Karnataka, India Introduction: Staphylococcal biofilms are prominent cause for acute and chronic infection both in hospital and community settings across the world. Current study explores biofilm formation by Staphylococcus aureus isolates from clinical samples by different methods. Methodology: Standard techniques used for the characterization of S.aureus. Qualitative and quantitative biofilm formation was assessed by Congo red Agar, Tube and Microtiter plate methods. Results: A total of 188 clinical isolates of S.aureus were screened for biofilm formation and 72 (38.29%) of them were found to be biofilm producers, 34 (18.08%) strong, 38 (20.21%) moderate. The remaining 116 (61.7%) were weak/ non biofilm producers. Maximum biofilm formers were recorded in pus samples (39.06%), followed by isolates from blood (38.23%) and urine (34.61%). Statistical analysis for the formation of biofilm indicated that Microtiter plate method is the most sensitive and specific method for screening biofilm production. Conclusions: Biofilm formation is one of the influential virulence factor in staphylococcal pathogenesis and persistence. Microtiter plate and Congo red agar remain as reliable methods for the qualitative and quantitative estimation of biofilm formation. Monitoring of biofilm formation in various etiological agents will help in determining the severity of infection. https://jidc.org/index.php/journal/article/view/10671BiofilmsStaphylococcus aureusbiofilm detection methods |
| spellingShingle | Alasthimannahalli Gangadhara Triveni Mendem Suresh Kumar Chavadi Manjunath Channappa T Shivannavar Subhaschandra M Gaddad Biofilm formation by clinically isolated Staphylococcus Aureus from India Journal of Infection in Developing Countries Biofilms Staphylococcus aureus biofilm detection methods |
| title | Biofilm formation by clinically isolated Staphylococcus Aureus from India |
| title_full | Biofilm formation by clinically isolated Staphylococcus Aureus from India |
| title_fullStr | Biofilm formation by clinically isolated Staphylococcus Aureus from India |
| title_full_unstemmed | Biofilm formation by clinically isolated Staphylococcus Aureus from India |
| title_short | Biofilm formation by clinically isolated Staphylococcus Aureus from India |
| title_sort | biofilm formation by clinically isolated staphylococcus aureus from india |
| topic | Biofilms Staphylococcus aureus biofilm detection methods |
| url | https://jidc.org/index.php/journal/article/view/10671 |
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