Cordyceps cicadae ameliorates inflammatory responses, oxidative stress, and fibrosis by targeting the PI3K/mTOR‐mediated autophagy pathway in the renal of MRL/lpr mice
Abstract Background The vast majority of systemic lupus erythematosus patients develop lupus nephritis (LN) with severe renal manifestations, such as inflammatory responses, oxidative stress, and fibrosis. The purpose of this research was to investigate Cordyceps cicadae as a potential therapeutic t...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-01-01
|
| Series: | Immunity, Inflammation and Disease |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/iid3.1168 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849423340682346496 |
|---|---|
| author | Feng Yang Yanyan Zhang Lei Dong Zhichao Song |
| author_facet | Feng Yang Yanyan Zhang Lei Dong Zhichao Song |
| author_sort | Feng Yang |
| collection | DOAJ |
| description | Abstract Background The vast majority of systemic lupus erythematosus patients develop lupus nephritis (LN) with severe renal manifestations, such as inflammatory responses, oxidative stress, and fibrosis. The purpose of this research was to investigate Cordyceps cicadae as a potential therapeutic target for treating inflammatory responses, oxidative stress, and fibrosis in LN. Methods The effects of C. cicadae on lupus symptoms in mice with LN were determined. MRL/lpr mice were treated with C. cicadae (4 g/kg/day, i.e., CC group, n = 8) or an equal volume of saline (model group, n = 8), and MRL/MP mice were treated with an equal volume of saline (control group, n = 8). Renal function indices, renal pathology, inflammatory markers, oxidative stress markers, and renal interstitial fibrosis levels were evaluated after C. cicadae treatment. Western blot analysis was performed to investigate the effect of C. cicadae on the expression of fibrosis biomarkers and the phosphatidylinositol 3‐kinase (PI3K)/mammalian target of rapamycin (mTOR)‐mediated autophagy pathway in the renal tissues of mice. Results C. cicadae ameliorated renal lesions, the inflammatory response, and oxidative stress damage in MRL/lpr mice. C. cicadae treatment inhibited renal fibrosis (16.31 ± 4.16 vs. 31.25 ± 5.61) and downregulated the expression of the fibrosis biomarkers alpha‐smooth muscle actin, fibronectin, and collagen I (COL I) in the kidneys of MRL/lpr mice. In addition, further research showed that the PI3K/mTOR‐mediated autophagy pathway was involved in C. cicadae‐mediated effects on renal fibrosis in MRL/lpr mice. Furthermore, the therapeutic effect of C. cicadae on repairing renal fibrosis and damage in MRL/lpr mice was abolished by the PI3K agonist 740 Y‐P. Conclusions The findings of the present research showed that C. cicadae could alleviate inflammatory responses, oxidative stress, and fibrosis in the renal tissues of mice with LN by targeting the PI3K/mTOR‐mediated autophagy pathway. |
| format | Article |
| id | doaj-art-a330cb6431bf4ed89bdbd2e080c88285 |
| institution | Kabale University |
| issn | 2050-4527 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Immunity, Inflammation and Disease |
| spelling | doaj-art-a330cb6431bf4ed89bdbd2e080c882852025-08-20T03:30:37ZengWileyImmunity, Inflammation and Disease2050-45272024-01-01121n/an/a10.1002/iid3.1168Cordyceps cicadae ameliorates inflammatory responses, oxidative stress, and fibrosis by targeting the PI3K/mTOR‐mediated autophagy pathway in the renal of MRL/lpr miceFeng Yang0Yanyan Zhang1Lei Dong2Zhichao Song3Department of Rheumatology Yantai Hospital of Traditional Chinese Medicine Yantai City Shandong ChinaDepartment of Rheumatology Yantai Hospital of Traditional Chinese Medicine Yantai City Shandong ChinaDepartment of Rheumatology Yantai Hospital of Traditional Chinese Medicine Yantai City Shandong ChinaDepartment of Rheumatology Yantai Hospital of Traditional Chinese Medicine Yantai City Shandong ChinaAbstract Background The vast majority of systemic lupus erythematosus patients develop lupus nephritis (LN) with severe renal manifestations, such as inflammatory responses, oxidative stress, and fibrosis. The purpose of this research was to investigate Cordyceps cicadae as a potential therapeutic target for treating inflammatory responses, oxidative stress, and fibrosis in LN. Methods The effects of C. cicadae on lupus symptoms in mice with LN were determined. MRL/lpr mice were treated with C. cicadae (4 g/kg/day, i.e., CC group, n = 8) or an equal volume of saline (model group, n = 8), and MRL/MP mice were treated with an equal volume of saline (control group, n = 8). Renal function indices, renal pathology, inflammatory markers, oxidative stress markers, and renal interstitial fibrosis levels were evaluated after C. cicadae treatment. Western blot analysis was performed to investigate the effect of C. cicadae on the expression of fibrosis biomarkers and the phosphatidylinositol 3‐kinase (PI3K)/mammalian target of rapamycin (mTOR)‐mediated autophagy pathway in the renal tissues of mice. Results C. cicadae ameliorated renal lesions, the inflammatory response, and oxidative stress damage in MRL/lpr mice. C. cicadae treatment inhibited renal fibrosis (16.31 ± 4.16 vs. 31.25 ± 5.61) and downregulated the expression of the fibrosis biomarkers alpha‐smooth muscle actin, fibronectin, and collagen I (COL I) in the kidneys of MRL/lpr mice. In addition, further research showed that the PI3K/mTOR‐mediated autophagy pathway was involved in C. cicadae‐mediated effects on renal fibrosis in MRL/lpr mice. Furthermore, the therapeutic effect of C. cicadae on repairing renal fibrosis and damage in MRL/lpr mice was abolished by the PI3K agonist 740 Y‐P. Conclusions The findings of the present research showed that C. cicadae could alleviate inflammatory responses, oxidative stress, and fibrosis in the renal tissues of mice with LN by targeting the PI3K/mTOR‐mediated autophagy pathway.https://doi.org/10.1002/iid3.1168autophagyCordyceps cicadaelupus nephritisPI3K/mTORrenal fibrosis |
| spellingShingle | Feng Yang Yanyan Zhang Lei Dong Zhichao Song Cordyceps cicadae ameliorates inflammatory responses, oxidative stress, and fibrosis by targeting the PI3K/mTOR‐mediated autophagy pathway in the renal of MRL/lpr mice Immunity, Inflammation and Disease autophagy Cordyceps cicadae lupus nephritis PI3K/mTOR renal fibrosis |
| title | Cordyceps cicadae ameliorates inflammatory responses, oxidative stress, and fibrosis by targeting the PI3K/mTOR‐mediated autophagy pathway in the renal of MRL/lpr mice |
| title_full | Cordyceps cicadae ameliorates inflammatory responses, oxidative stress, and fibrosis by targeting the PI3K/mTOR‐mediated autophagy pathway in the renal of MRL/lpr mice |
| title_fullStr | Cordyceps cicadae ameliorates inflammatory responses, oxidative stress, and fibrosis by targeting the PI3K/mTOR‐mediated autophagy pathway in the renal of MRL/lpr mice |
| title_full_unstemmed | Cordyceps cicadae ameliorates inflammatory responses, oxidative stress, and fibrosis by targeting the PI3K/mTOR‐mediated autophagy pathway in the renal of MRL/lpr mice |
| title_short | Cordyceps cicadae ameliorates inflammatory responses, oxidative stress, and fibrosis by targeting the PI3K/mTOR‐mediated autophagy pathway in the renal of MRL/lpr mice |
| title_sort | cordyceps cicadae ameliorates inflammatory responses oxidative stress and fibrosis by targeting the pi3k mtor mediated autophagy pathway in the renal of mrl lpr mice |
| topic | autophagy Cordyceps cicadae lupus nephritis PI3K/mTOR renal fibrosis |
| url | https://doi.org/10.1002/iid3.1168 |
| work_keys_str_mv | AT fengyang cordycepscicadaeamelioratesinflammatoryresponsesoxidativestressandfibrosisbytargetingthepi3kmtormediatedautophagypathwayintherenalofmrllprmice AT yanyanzhang cordycepscicadaeamelioratesinflammatoryresponsesoxidativestressandfibrosisbytargetingthepi3kmtormediatedautophagypathwayintherenalofmrllprmice AT leidong cordycepscicadaeamelioratesinflammatoryresponsesoxidativestressandfibrosisbytargetingthepi3kmtormediatedautophagypathwayintherenalofmrllprmice AT zhichaosong cordycepscicadaeamelioratesinflammatoryresponsesoxidativestressandfibrosisbytargetingthepi3kmtormediatedautophagypathwayintherenalofmrllprmice |