Evaluation of Exogenous Proteases in Broiler Diets

ABSTRACT This study aimed to evaluate the effects of different exogenous proteases in broiler diets on performance, metabolizable energy, and the crude protein (CP) digestibility values of diets. A total of 1440, 384, and 432 birds were distributed in a completely randomized design in the performanc...

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Main Authors: BF Almeida, CH Oliveira, KMM Dias, RD Bernardes, SO Borges, KM Gomes, LP Castro, RV Nunes, AA Calderano
Format: Article
Language:English
Published: Fundação APINCO de Ciência e Tecnologia Avícolas 2025-05-01
Series:Brazilian Journal of Poultry Science
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2025000100315&lng=en&tlng=en
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author BF Almeida
CH Oliveira
KMM Dias
RD Bernardes
SO Borges
KM Gomes
LP Castro
RV Nunes
AA Calderano
author_facet BF Almeida
CH Oliveira
KMM Dias
RD Bernardes
SO Borges
KM Gomes
LP Castro
RV Nunes
AA Calderano
author_sort BF Almeida
collection DOAJ
description ABSTRACT This study aimed to evaluate the effects of different exogenous proteases in broiler diets on performance, metabolizable energy, and the crude protein (CP) digestibility values of diets. A total of 1440, 384, and 432 birds were distributed in a completely randomized design in the performance, metabolism, and digestibility experiments, respectively. The treatments applied to the three experiments were: T1 - positive control (PC; basal diet, 23,23% CP in starter phase and 20,58% CP in grower/finisher phase); T2 - negative control (NC; 18,54% CP in starter phase and 18,46% CP in grower/finisher phase); T3 - NC added with Protease A; T4 - NC added with Protease B; T5 - NC added with Protease C; T6 - NC added with Protease D; T7 - NC added with Protease E; and T8 - NC added with Protease F, plus a treatment with a protein-free diet in the digestibility experiment. Reducing the protein level in the diet reduced BWG and worsened FCR. The proteases did not improve any of the performance parameters evaluated, except for protease C, which improved BWG, but not at a sufficient level to recover the lost performance, and not equal to CP. In addition, there were no differences in the metabolizable energy values. Furthermore, the proteases did not improve the standardized ileal digestibility of crude protein of diets with reduced protein. In conclusion, the supplementation of the tested proteases does not recover the growth performance lost by reducing the crude protein in the diet.
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spelling doaj-art-a314e071d141421da5ee8fc554a6f2f72025-08-20T02:24:24ZengFundação APINCO de Ciência e Tecnologia AvícolasBrazilian Journal of Poultry Science1806-90612025-05-0127110.1590/1806-9061-2024-1981Evaluation of Exogenous Proteases in Broiler DietsBF Almeidahttps://orcid.org/0000-0003-1894-0562CH Oliveirahttps://orcid.org/0000-0001-8054-5550KMM Diashttps://orcid.org/0000-0002-1757-3011RD Bernardeshttps://orcid.org/0000-0001-7013-0650SO Borgeshttps://orcid.org/0000-0002-7695-7760KM Gomeshttps://orcid.org/0000-0002-2180-5209LP Castrohttps://orcid.org/0000-0003-4501-9226RV Nuneshttps://orcid.org/0000-0002-9376-2826AA Calderanohttps://orcid.org/0000-0002-2282-3580ABSTRACT This study aimed to evaluate the effects of different exogenous proteases in broiler diets on performance, metabolizable energy, and the crude protein (CP) digestibility values of diets. A total of 1440, 384, and 432 birds were distributed in a completely randomized design in the performance, metabolism, and digestibility experiments, respectively. The treatments applied to the three experiments were: T1 - positive control (PC; basal diet, 23,23% CP in starter phase and 20,58% CP in grower/finisher phase); T2 - negative control (NC; 18,54% CP in starter phase and 18,46% CP in grower/finisher phase); T3 - NC added with Protease A; T4 - NC added with Protease B; T5 - NC added with Protease C; T6 - NC added with Protease D; T7 - NC added with Protease E; and T8 - NC added with Protease F, plus a treatment with a protein-free diet in the digestibility experiment. Reducing the protein level in the diet reduced BWG and worsened FCR. The proteases did not improve any of the performance parameters evaluated, except for protease C, which improved BWG, but not at a sufficient level to recover the lost performance, and not equal to CP. In addition, there were no differences in the metabolizable energy values. Furthermore, the proteases did not improve the standardized ileal digestibility of crude protein of diets with reduced protein. In conclusion, the supplementation of the tested proteases does not recover the growth performance lost by reducing the crude protein in the diet.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2025000100315&lng=en&tlng=enPoultryperformancelow-protein dietprotease
spellingShingle BF Almeida
CH Oliveira
KMM Dias
RD Bernardes
SO Borges
KM Gomes
LP Castro
RV Nunes
AA Calderano
Evaluation of Exogenous Proteases in Broiler Diets
Brazilian Journal of Poultry Science
Poultry
performance
low-protein diet
protease
title Evaluation of Exogenous Proteases in Broiler Diets
title_full Evaluation of Exogenous Proteases in Broiler Diets
title_fullStr Evaluation of Exogenous Proteases in Broiler Diets
title_full_unstemmed Evaluation of Exogenous Proteases in Broiler Diets
title_short Evaluation of Exogenous Proteases in Broiler Diets
title_sort evaluation of exogenous proteases in broiler diets
topic Poultry
performance
low-protein diet
protease
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2025000100315&lng=en&tlng=en
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AT rdbernardes evaluationofexogenousproteasesinbroilerdiets
AT soborges evaluationofexogenousproteasesinbroilerdiets
AT kmgomes evaluationofexogenousproteasesinbroilerdiets
AT lpcastro evaluationofexogenousproteasesinbroilerdiets
AT rvnunes evaluationofexogenousproteasesinbroilerdiets
AT aacalderano evaluationofexogenousproteasesinbroilerdiets