Resistance phenotypes and genomic features of Mycobacterium seoulense isolates
BackgroundMycobacterium seoulense (M. seoulense) is an emerging pathogen increasingly associated with infections; however, its resistance phenotypes and genomic characteristics remain largely unknown.MethodsSeven M. seoulense isolates were collected from clinical samples. Drug susceptibility testing...
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Frontiers Media S.A.
2025-04-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1553591/full |
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| author | Jin Zhao Xinli Shen Lulu Jin Songjun Ji Xinling Pan |
| author_facet | Jin Zhao Xinli Shen Lulu Jin Songjun Ji Xinling Pan |
| author_sort | Jin Zhao |
| collection | DOAJ |
| description | BackgroundMycobacterium seoulense (M. seoulense) is an emerging pathogen increasingly associated with infections; however, its resistance phenotypes and genomic characteristics remain largely unknown.MethodsSeven M. seoulense isolates were collected from clinical samples. Drug susceptibility testing was conducted using Sensititre™ SLOMYCO2 susceptibility plates. Whole genome sequencing and supporting bioinformatics analyses were performed to analyze the genomic features.ResultsAll M. seoulense isolates (n=7) exhibited growth on 7H10 agar medium containing thiophenecarboxylic acid hydrazide or p-Nitrobenzoic acid, with marked diversity in growth rates in liquid culture. All strains exhibited high minimum inhibitor concentrations (MICs) for minocycline (>8 μg/mL), doxycycline (>8 μg/mL), and amikacin (16-32 μg/mL). The MICs for linezolid, rifabutin, moxifloxacin, ciprofloxacin, streptomycin, clarithromycin, and rifampicin varied among the isolates. High levels of genomic diversity were noted among these strains concerning genome-called single nucleotide polymorphisms and average nucleotide identity. In total, 4,282 genes were shared across all genomes, while 315 unique genes were restricted to one strain. Comparative genomic analysis identified two unique virulence genes encoding a catalase enzyme and a protein involved in capsule biosynthesis and transport. Additionally, all M. seoulense strains demonstrated the ability to survive within macrophages.ConclusionThe clinical M. seoulense isolates analyzed in this study exhibited varying levels of antibiotic susceptibility, suggesting the potential need for susceptibility testing to guide clinical treatment. Genomic features of these isolates indicated that they are likely pathogenic non-tuberculous mycobacterium, highlighting a need for closer epidemiological monitoring. |
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| language | English |
| publishDate | 2025-04-01 |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-a313ea1e57b84029bd04327d427be9f12025-08-20T02:25:43ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-04-011510.3389/fcimb.2025.15535911553591Resistance phenotypes and genomic features of Mycobacterium seoulense isolatesJin ZhaoXinli ShenLulu JinSongjun JiXinling PanBackgroundMycobacterium seoulense (M. seoulense) is an emerging pathogen increasingly associated with infections; however, its resistance phenotypes and genomic characteristics remain largely unknown.MethodsSeven M. seoulense isolates were collected from clinical samples. Drug susceptibility testing was conducted using Sensititre™ SLOMYCO2 susceptibility plates. Whole genome sequencing and supporting bioinformatics analyses were performed to analyze the genomic features.ResultsAll M. seoulense isolates (n=7) exhibited growth on 7H10 agar medium containing thiophenecarboxylic acid hydrazide or p-Nitrobenzoic acid, with marked diversity in growth rates in liquid culture. All strains exhibited high minimum inhibitor concentrations (MICs) for minocycline (>8 μg/mL), doxycycline (>8 μg/mL), and amikacin (16-32 μg/mL). The MICs for linezolid, rifabutin, moxifloxacin, ciprofloxacin, streptomycin, clarithromycin, and rifampicin varied among the isolates. High levels of genomic diversity were noted among these strains concerning genome-called single nucleotide polymorphisms and average nucleotide identity. In total, 4,282 genes were shared across all genomes, while 315 unique genes were restricted to one strain. Comparative genomic analysis identified two unique virulence genes encoding a catalase enzyme and a protein involved in capsule biosynthesis and transport. Additionally, all M. seoulense strains demonstrated the ability to survive within macrophages.ConclusionThe clinical M. seoulense isolates analyzed in this study exhibited varying levels of antibiotic susceptibility, suggesting the potential need for susceptibility testing to guide clinical treatment. Genomic features of these isolates indicated that they are likely pathogenic non-tuberculous mycobacterium, highlighting a need for closer epidemiological monitoring.https://www.frontiersin.org/articles/10.3389/fcimb.2025.1553591/fullM. seoulensewhole genome sequencingvirulence factordrug susceptibilityintracellular survival |
| spellingShingle | Jin Zhao Xinli Shen Lulu Jin Songjun Ji Xinling Pan Resistance phenotypes and genomic features of Mycobacterium seoulense isolates Frontiers in Cellular and Infection Microbiology M. seoulense whole genome sequencing virulence factor drug susceptibility intracellular survival |
| title | Resistance phenotypes and genomic features of Mycobacterium seoulense isolates |
| title_full | Resistance phenotypes and genomic features of Mycobacterium seoulense isolates |
| title_fullStr | Resistance phenotypes and genomic features of Mycobacterium seoulense isolates |
| title_full_unstemmed | Resistance phenotypes and genomic features of Mycobacterium seoulense isolates |
| title_short | Resistance phenotypes and genomic features of Mycobacterium seoulense isolates |
| title_sort | resistance phenotypes and genomic features of mycobacterium seoulense isolates |
| topic | M. seoulense whole genome sequencing virulence factor drug susceptibility intracellular survival |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1553591/full |
| work_keys_str_mv | AT jinzhao resistancephenotypesandgenomicfeaturesofmycobacteriumseoulenseisolates AT xinlishen resistancephenotypesandgenomicfeaturesofmycobacteriumseoulenseisolates AT lulujin resistancephenotypesandgenomicfeaturesofmycobacteriumseoulenseisolates AT songjunji resistancephenotypesandgenomicfeaturesofmycobacteriumseoulenseisolates AT xinlingpan resistancephenotypesandgenomicfeaturesofmycobacteriumseoulenseisolates |