Clinical Factors Influencing Phenotype of HCMV-Specific CD8+ T Cells and HCMV-Induced Interferon-Gamma Production after Allogeneic Stem Cells Transplantation
Human cytomegalovirus (HCMV) infection causes significant morbidity and mortality after hematopoietic stem cell transplantation (HSCT). In this work, we characterized the phenotype and interferon-gamma (INF-γ) production of HCMV-specific T cells using QuantiFERON-HCMV assay in 26 patients 6 months a...
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| Format: | Article |
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Wiley
2013-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2013/347213 |
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| author | Inmaculada Gayoso Sara Cantisán Carolina Cerrato Joaquín Sánchez-García Carmen Martin Rafael Solana Antonio Torres-Gomez Julian Torre-Cisneros |
| author_facet | Inmaculada Gayoso Sara Cantisán Carolina Cerrato Joaquín Sánchez-García Carmen Martin Rafael Solana Antonio Torres-Gomez Julian Torre-Cisneros |
| author_sort | Inmaculada Gayoso |
| collection | DOAJ |
| description | Human cytomegalovirus (HCMV) infection causes significant morbidity and mortality after hematopoietic stem cell transplantation (HSCT). In this work, we characterized the phenotype and interferon-gamma (INF-γ) production of HCMV-specific T cells using QuantiFERON-HCMV assay in 26 patients 6 months after HSCT. We analysed whether these two parameters were associated with clinical variables. Our results showed that the patients receiving stem cells from donors ≥40 years old were 12 times more likely to have HCMV-specific CD8+ T cells with “differentiated phenotype” (CD45RA+CCR7+ ≤6.7% and CD28+ ≤30%) than patients grafted from donors <40 years old (OR=12; P=0.014). In addition, a detectable IFN-γ production in response to HCMV peptides (cutoff 0.2 IU/mL IFN-γ; “reactive” QuantiFERON-HCMV test) was statistically associated with HCMV replication after transplantation (OR=11; P=0.026), recipients ≥40 versus <40 years old (OR=11; P=0.026), and the use of peripheral blood versus bone marrow as stem cell source (OR=17.5; P=0.024). In conclusion, donor age is the only factor significantly associated with the presence of the “differentiated phenotype” in HCMV-specific CD8+ T cells, whereas HCMV replication after transplantation, recipient age, and stem cell source are the factors associated with the production of IFN-γ in response to HCMV epitopes. |
| format | Article |
| id | doaj-art-a310bc398d784dc9a691eff7a56be619 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-a310bc398d784dc9a691eff7a56be6192025-02-03T01:09:23ZengWileyClinical and Developmental Immunology1740-25221740-25302013-01-01201310.1155/2013/347213347213Clinical Factors Influencing Phenotype of HCMV-Specific CD8+ T Cells and HCMV-Induced Interferon-Gamma Production after Allogeneic Stem Cells TransplantationInmaculada Gayoso0Sara Cantisán1Carolina Cerrato2Joaquín Sánchez-García3Carmen Martin4Rafael Solana5Antonio Torres-Gomez6Julian Torre-Cisneros7Instituto Maimonides para la Investigacion Biomedica de Córdoba (IMIBIC), Reina Sofia University Hospital, 14004 Cordoba, SpainInstituto Maimonides para la Investigacion Biomedica de Córdoba (IMIBIC), Reina Sofia University Hospital, 14004 Cordoba, SpainInstituto Maimonides para la Investigacion Biomedica de Córdoba (IMIBIC), Reina Sofia University Hospital, 14004 Cordoba, SpainInstituto Maimonides para la Investigacion Biomedica de Córdoba (IMIBIC), Reina Sofia University Hospital, 14004 Cordoba, SpainInstituto Maimonides para la Investigacion Biomedica de Córdoba (IMIBIC), Reina Sofia University Hospital, 14004 Cordoba, SpainInstituto Maimonides para la Investigacion Biomedica de Córdoba (IMIBIC), Reina Sofia University Hospital, 14004 Cordoba, SpainInstituto Maimonides para la Investigacion Biomedica de Córdoba (IMIBIC), Reina Sofia University Hospital, 14004 Cordoba, SpainInstituto Maimonides para la Investigacion Biomedica de Córdoba (IMIBIC), Reina Sofia University Hospital, 14004 Cordoba, SpainHuman cytomegalovirus (HCMV) infection causes significant morbidity and mortality after hematopoietic stem cell transplantation (HSCT). In this work, we characterized the phenotype and interferon-gamma (INF-γ) production of HCMV-specific T cells using QuantiFERON-HCMV assay in 26 patients 6 months after HSCT. We analysed whether these two parameters were associated with clinical variables. Our results showed that the patients receiving stem cells from donors ≥40 years old were 12 times more likely to have HCMV-specific CD8+ T cells with “differentiated phenotype” (CD45RA+CCR7+ ≤6.7% and CD28+ ≤30%) than patients grafted from donors <40 years old (OR=12; P=0.014). In addition, a detectable IFN-γ production in response to HCMV peptides (cutoff 0.2 IU/mL IFN-γ; “reactive” QuantiFERON-HCMV test) was statistically associated with HCMV replication after transplantation (OR=11; P=0.026), recipients ≥40 versus <40 years old (OR=11; P=0.026), and the use of peripheral blood versus bone marrow as stem cell source (OR=17.5; P=0.024). In conclusion, donor age is the only factor significantly associated with the presence of the “differentiated phenotype” in HCMV-specific CD8+ T cells, whereas HCMV replication after transplantation, recipient age, and stem cell source are the factors associated with the production of IFN-γ in response to HCMV epitopes.http://dx.doi.org/10.1155/2013/347213 |
| spellingShingle | Inmaculada Gayoso Sara Cantisán Carolina Cerrato Joaquín Sánchez-García Carmen Martin Rafael Solana Antonio Torres-Gomez Julian Torre-Cisneros Clinical Factors Influencing Phenotype of HCMV-Specific CD8+ T Cells and HCMV-Induced Interferon-Gamma Production after Allogeneic Stem Cells Transplantation Clinical and Developmental Immunology |
| title | Clinical Factors Influencing Phenotype of HCMV-Specific CD8+ T Cells and HCMV-Induced Interferon-Gamma Production after Allogeneic Stem Cells Transplantation |
| title_full | Clinical Factors Influencing Phenotype of HCMV-Specific CD8+ T Cells and HCMV-Induced Interferon-Gamma Production after Allogeneic Stem Cells Transplantation |
| title_fullStr | Clinical Factors Influencing Phenotype of HCMV-Specific CD8+ T Cells and HCMV-Induced Interferon-Gamma Production after Allogeneic Stem Cells Transplantation |
| title_full_unstemmed | Clinical Factors Influencing Phenotype of HCMV-Specific CD8+ T Cells and HCMV-Induced Interferon-Gamma Production after Allogeneic Stem Cells Transplantation |
| title_short | Clinical Factors Influencing Phenotype of HCMV-Specific CD8+ T Cells and HCMV-Induced Interferon-Gamma Production after Allogeneic Stem Cells Transplantation |
| title_sort | clinical factors influencing phenotype of hcmv specific cd8 t cells and hcmv induced interferon gamma production after allogeneic stem cells transplantation |
| url | http://dx.doi.org/10.1155/2013/347213 |
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