Avian Haemosporidian Parasites in Three Wild Columbids from Germany

Birds are hosts to a diverse assemblage of vector-transmitted haemosporidian parasites. However, the true genetic diversity and many host–parasite interactions are still unknown, particularly in under-represented species groups such as wild doves and pigeons (Columbiformes). In this study, we examin...

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Main Authors: Yvonne R. Schumm, Celine Frank, Uta Gerz, Hannes Ruß, Benjamin Metzger, Petra Quillfeldt
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Microorganisms
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Online Access:https://www.mdpi.com/2076-2607/13/6/1305
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author Yvonne R. Schumm
Celine Frank
Uta Gerz
Hannes Ruß
Benjamin Metzger
Petra Quillfeldt
author_facet Yvonne R. Schumm
Celine Frank
Uta Gerz
Hannes Ruß
Benjamin Metzger
Petra Quillfeldt
author_sort Yvonne R. Schumm
collection DOAJ
description Birds are hosts to a diverse assemblage of vector-transmitted haemosporidian parasites. However, the true genetic diversity and many host–parasite interactions are still unknown, particularly in under-represented species groups such as wild doves and pigeons (Columbiformes). In this study, we examined the prevalence and lineage diversity of haemosporidian genera <i>Plasmodium</i>, <i>Leucocytozoon</i>, and <i>Haemoproteus</i> in three species of wild columbids, sampled in Germany. Examinations were performed by applying molecular methods (nested PCR and one-step multiplex PCR) and blood smear examination, and their respective advantages and disadvantages are discussed. In the case of the European Turtle Dove <i>Streptopelia turtur</i>, samples were collected along a west–east gradient throughout Germany, covering migratory birds from the Western and Central-Eastern flyway of this species. Although no infection was detected in the Stock Dove <i>Columba oenas</i> samples, 53% of Turtle Dove and 86% of Common Woodpigeon <i>Columba palumbus</i> harbored a parasite of at least one haemosporidian genus, revealing previously unknown lineage–host interactions. We were not able to demonstrate a correlation between infection status (presence or absence of infection based on PCR results) and parasitemia with condition based on the heterophil to lymphocyte ratio (H/L ratio). Neither lineage occurrence nor prevalence of infection followed any geographically specific patterns. Thus, haemosporidian lineages found in Turtle Doves could not be used as a marker of geographic origin that would allow the tracking of their nonbreeding distribution.
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spelling doaj-art-a30b5be53bd44bb881fb8772ae89cfb72025-08-20T02:21:06ZengMDPI AGMicroorganisms2076-26072025-06-01136130510.3390/microorganisms13061305Avian Haemosporidian Parasites in Three Wild Columbids from GermanyYvonne R. Schumm0Celine Frank1Uta Gerz2Hannes Ruß3Benjamin Metzger4Petra Quillfeldt5Department of Animal Ecology & Systematics, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392 Giessen, GermanyDepartment of Animal Ecology & Systematics, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392 Giessen, GermanyDepartment of Animal Ecology & Systematics, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392 Giessen, GermanyDepartment of Animal Ecology & Systematics, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392 Giessen, GermanyIndependent Researcher, 26/1 Immaculate Conception Street, GZR 1141 Gzira, MaltaDepartment of Animal Ecology & Systematics, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392 Giessen, GermanyBirds are hosts to a diverse assemblage of vector-transmitted haemosporidian parasites. However, the true genetic diversity and many host–parasite interactions are still unknown, particularly in under-represented species groups such as wild doves and pigeons (Columbiformes). In this study, we examined the prevalence and lineage diversity of haemosporidian genera <i>Plasmodium</i>, <i>Leucocytozoon</i>, and <i>Haemoproteus</i> in three species of wild columbids, sampled in Germany. Examinations were performed by applying molecular methods (nested PCR and one-step multiplex PCR) and blood smear examination, and their respective advantages and disadvantages are discussed. In the case of the European Turtle Dove <i>Streptopelia turtur</i>, samples were collected along a west–east gradient throughout Germany, covering migratory birds from the Western and Central-Eastern flyway of this species. Although no infection was detected in the Stock Dove <i>Columba oenas</i> samples, 53% of Turtle Dove and 86% of Common Woodpigeon <i>Columba palumbus</i> harbored a parasite of at least one haemosporidian genus, revealing previously unknown lineage–host interactions. We were not able to demonstrate a correlation between infection status (presence or absence of infection based on PCR results) and parasitemia with condition based on the heterophil to lymphocyte ratio (H/L ratio). Neither lineage occurrence nor prevalence of infection followed any geographically specific patterns. Thus, haemosporidian lineages found in Turtle Doves could not be used as a marker of geographic origin that would allow the tracking of their nonbreeding distribution.https://www.mdpi.com/2076-2607/13/6/1305avian hostblood parasite infectionH/L ratiohaemosporidian lineageEuropean Turtle Dove
spellingShingle Yvonne R. Schumm
Celine Frank
Uta Gerz
Hannes Ruß
Benjamin Metzger
Petra Quillfeldt
Avian Haemosporidian Parasites in Three Wild Columbids from Germany
Microorganisms
avian host
blood parasite infection
H/L ratio
haemosporidian lineage
European Turtle Dove
title Avian Haemosporidian Parasites in Three Wild Columbids from Germany
title_full Avian Haemosporidian Parasites in Three Wild Columbids from Germany
title_fullStr Avian Haemosporidian Parasites in Three Wild Columbids from Germany
title_full_unstemmed Avian Haemosporidian Parasites in Three Wild Columbids from Germany
title_short Avian Haemosporidian Parasites in Three Wild Columbids from Germany
title_sort avian haemosporidian parasites in three wild columbids from germany
topic avian host
blood parasite infection
H/L ratio
haemosporidian lineage
European Turtle Dove
url https://www.mdpi.com/2076-2607/13/6/1305
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