Resistance profile of Escherichia coli strains in micro-basins with different soil use and occupation, in the Northwest of Rio Grande do Sul, Brazil
Escherichia coli, a highly adaptive microorganism, has gained scientific interest due to its rapid mutation and resistance to antimicrobial treatments. The primary sources that contribute to its rapid spread and the emergence of infections remain hotly debated. Thus, the objective of this research w...
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Associação Brasileira de Engenharia Sanitária e Ambiental
2025-04-01
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| Series: | Revista Brasileira de Ciências Ambientais |
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| Online Access: | https://www.rbciamb.com.br/Publicacoes_RBCIAMB/article/view/2175 |
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| author | Ubiratan Alegransi Bones Genesio Mario da Rosa Kauane Andressa Flach Jefferson Alves da Costa Junior Nelson Emanoel Freitas Endres |
| author_facet | Ubiratan Alegransi Bones Genesio Mario da Rosa Kauane Andressa Flach Jefferson Alves da Costa Junior Nelson Emanoel Freitas Endres |
| author_sort | Ubiratan Alegransi Bones |
| collection | DOAJ |
| description | Escherichia coli, a highly adaptive microorganism, has gained scientific interest due to its rapid mutation and resistance to antimicrobial treatments. The primary sources that contribute to its rapid spread and the emergence of infections remain hotly debated. Thus, the objective of this research was to provide a baseline, as the first study in the region that analyzes the microbiological quality of water in micro-basins with different characteristics of land use and occupation. Intending to identify whether there is resistance in the isolated strains of E. coli and what their possible origins are, the study provides a behavior modeling of the studied area. To this end, three sampling campaigns were carried out, from which these microorganisms were isolated and identified by polymerase chain reaction, and were subsequently subjected to susceptibility tests with nine antibacterials widely used in the treatment of infections caused by this bacterial species. Theresultsrevealed that the watershed receiving urban sanitary effluents presented the highest contamination levels, with persistent multidrug-resistant strains throughout the collection period. Among all strains, 80% were resistant to ampicillin, while 33.3% showed resistance to cefazolin. Levofloxacinwas the most effective antibiotic, with 100% susceptibility. Micro-basins with predominant agricultural and pasture activities demonstrated less resistance than urban-impacted areas. Thishighlights the urgent need for constant monitoring and further studies to uncover the main determinants of this urgent ecological and public health problem.
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| format | Article |
| id | doaj-art-a307f03ec2fb40dd8ee16d2e9c3c3d70 |
| institution | DOAJ |
| issn | 1808-4524 2176-9478 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Associação Brasileira de Engenharia Sanitária e Ambiental |
| record_format | Article |
| series | Revista Brasileira de Ciências Ambientais |
| spelling | doaj-art-a307f03ec2fb40dd8ee16d2e9c3c3d702025-08-20T03:10:32ZengAssociação Brasileira de Engenharia Sanitária e AmbientalRevista Brasileira de Ciências Ambientais1808-45242176-94782025-04-0160e2175e217510.5327/Z2176-947821751822Resistance profile of Escherichia coli strains in micro-basins with different soil use and occupation, in the Northwest of Rio Grande do Sul, BrazilUbiratan Alegransi Bones0https://orcid.org/0000-0002-2220-5686Genesio Mario da Rosa1https://orcid.org/0000-0003-1247-2286Kauane Andressa Flach2https://orcid.org/0000-0001-8915-3289Jefferson Alves da Costa Junior3https://orcid.org/0000-0001-7572-3930Nelson Emanoel Freitas Endres4https://orcid.org/0009-0005-0574-1267Universidade Federal de Santa Maria (UFSM) - BrazilUniversidade Federal de Santa Maria (UFSM) - BrazilUniversidade Federal de Santa Maria (UFSM) - BrazilUniversidade Federal de Santa Maria (UFSM) - BrazilUniversidade Federal de Santa Maria (UFSM) - BrazilEscherichia coli, a highly adaptive microorganism, has gained scientific interest due to its rapid mutation and resistance to antimicrobial treatments. The primary sources that contribute to its rapid spread and the emergence of infections remain hotly debated. Thus, the objective of this research was to provide a baseline, as the first study in the region that analyzes the microbiological quality of water in micro-basins with different characteristics of land use and occupation. Intending to identify whether there is resistance in the isolated strains of E. coli and what their possible origins are, the study provides a behavior modeling of the studied area. To this end, three sampling campaigns were carried out, from which these microorganisms were isolated and identified by polymerase chain reaction, and were subsequently subjected to susceptibility tests with nine antibacterials widely used in the treatment of infections caused by this bacterial species. Theresultsrevealed that the watershed receiving urban sanitary effluents presented the highest contamination levels, with persistent multidrug-resistant strains throughout the collection period. Among all strains, 80% were resistant to ampicillin, while 33.3% showed resistance to cefazolin. Levofloxacinwas the most effective antibiotic, with 100% susceptibility. Micro-basins with predominant agricultural and pasture activities demonstrated less resistance than urban-impacted areas. Thishighlights the urgent need for constant monitoring and further studies to uncover the main determinants of this urgent ecological and public health problem. .https://www.rbciamb.com.br/Publicacoes_RBCIAMB/article/view/2175bacterial resistance; water contamination; sanitary effluents |
| spellingShingle | Ubiratan Alegransi Bones Genesio Mario da Rosa Kauane Andressa Flach Jefferson Alves da Costa Junior Nelson Emanoel Freitas Endres Resistance profile of Escherichia coli strains in micro-basins with different soil use and occupation, in the Northwest of Rio Grande do Sul, Brazil Revista Brasileira de Ciências Ambientais bacterial resistance; water contamination; sanitary effluents |
| title | Resistance profile of Escherichia coli strains in micro-basins with different soil use and occupation, in the Northwest of Rio Grande do Sul, Brazil |
| title_full | Resistance profile of Escherichia coli strains in micro-basins with different soil use and occupation, in the Northwest of Rio Grande do Sul, Brazil |
| title_fullStr | Resistance profile of Escherichia coli strains in micro-basins with different soil use and occupation, in the Northwest of Rio Grande do Sul, Brazil |
| title_full_unstemmed | Resistance profile of Escherichia coli strains in micro-basins with different soil use and occupation, in the Northwest of Rio Grande do Sul, Brazil |
| title_short | Resistance profile of Escherichia coli strains in micro-basins with different soil use and occupation, in the Northwest of Rio Grande do Sul, Brazil |
| title_sort | resistance profile of escherichia coli strains in micro basins with different soil use and occupation in the northwest of rio grande do sul brazil |
| topic | bacterial resistance; water contamination; sanitary effluents |
| url | https://www.rbciamb.com.br/Publicacoes_RBCIAMB/article/view/2175 |
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