Evaluation of the Selenotranscriptome Expression in Two Hepatocellular Carcinoma Cell Lines
Hepatocellular carcinoma (HCC) is the most common type of liver cancer and is still one of the most fatal cancers. Hence, it needs to identify always new putative markers to improve its diagnosis and prognosis. Since the selenium is able to fight the oxidative damage which is one of the major origin...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2015/419561 |
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| author | Stefano Guariniello Giovanni Di Bernardo Giovanni Colonna Marcella Cammarota Giuseppe Castello Susan Costantini |
| author_facet | Stefano Guariniello Giovanni Di Bernardo Giovanni Colonna Marcella Cammarota Giuseppe Castello Susan Costantini |
| author_sort | Stefano Guariniello |
| collection | DOAJ |
| description | Hepatocellular carcinoma (HCC) is the most common type of liver cancer and is still one of the most fatal cancers. Hence, it needs to identify always new putative markers to improve its diagnosis and prognosis. Since the selenium is able to fight the oxidative damage which is one of the major origins of cell damage as well as cancer, we have recently focused our attention on selenoprotein family and their involvement in HCC. In the present paper we have carried out a global analysis of the selenotranscriptome expression in HepG2 and Huh7 cells compared to the normal human hepatocytes by reverse transcription-qPCR (RT-qPCR). Our data showed that in both cells there are three downregulated (DIO1, DIO2, and SELO) and ten upregulated (GPX4, GPX7, SELK, SELM, SELN, SELT, SELV, SEP15, SEPW1, and TrxR1) genes. Additionally, interactomic studies were carried out to evaluate the ability of these down- and upregulated genes to interact between them as well as to identify putative HUB nodes representing the centers of correlation able to exercise a direct control over the coordinated genes. |
| format | Article |
| id | doaj-art-a2fd5de799254fb3a112a98f2dae9d8c |
| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-a2fd5de799254fb3a112a98f2dae9d8c2025-02-03T05:49:33ZengWileyAnalytical Cellular Pathology2210-71772210-71852015-01-01201510.1155/2015/419561419561Evaluation of the Selenotranscriptome Expression in Two Hepatocellular Carcinoma Cell LinesStefano Guariniello0Giovanni Di Bernardo1Giovanni Colonna2Marcella Cammarota3Giuseppe Castello4Susan Costantini5Dipartimento di Biochimica, Biofisica e Patologia Generale, Seconda Università degli Studi di Napoli, 80138 Napoli, ItalyDipartimento di Medicina Sperimentale, Seconda Università degli Studi di Napoli, 80138 Napoli, ItalyServizio di Informatica Medica, Azienda Ospedaliera Universitaria, Seconda Università di Napoli, 80138 Napoli, ItalyDipartimento di Medicina Sperimentale, Seconda Università degli Studi di Napoli, 80138 Napoli, ItalyCROM, Istituto Nazionale Tumori “Fondazione G. Pascale”, IRCCS, 80131 Napoli, ItalyCROM, Istituto Nazionale Tumori “Fondazione G. Pascale”, IRCCS, 80131 Napoli, ItalyHepatocellular carcinoma (HCC) is the most common type of liver cancer and is still one of the most fatal cancers. Hence, it needs to identify always new putative markers to improve its diagnosis and prognosis. Since the selenium is able to fight the oxidative damage which is one of the major origins of cell damage as well as cancer, we have recently focused our attention on selenoprotein family and their involvement in HCC. In the present paper we have carried out a global analysis of the selenotranscriptome expression in HepG2 and Huh7 cells compared to the normal human hepatocytes by reverse transcription-qPCR (RT-qPCR). Our data showed that in both cells there are three downregulated (DIO1, DIO2, and SELO) and ten upregulated (GPX4, GPX7, SELK, SELM, SELN, SELT, SELV, SEP15, SEPW1, and TrxR1) genes. Additionally, interactomic studies were carried out to evaluate the ability of these down- and upregulated genes to interact between them as well as to identify putative HUB nodes representing the centers of correlation able to exercise a direct control over the coordinated genes.http://dx.doi.org/10.1155/2015/419561 |
| spellingShingle | Stefano Guariniello Giovanni Di Bernardo Giovanni Colonna Marcella Cammarota Giuseppe Castello Susan Costantini Evaluation of the Selenotranscriptome Expression in Two Hepatocellular Carcinoma Cell Lines Analytical Cellular Pathology |
| title | Evaluation of the Selenotranscriptome Expression in Two Hepatocellular Carcinoma Cell Lines |
| title_full | Evaluation of the Selenotranscriptome Expression in Two Hepatocellular Carcinoma Cell Lines |
| title_fullStr | Evaluation of the Selenotranscriptome Expression in Two Hepatocellular Carcinoma Cell Lines |
| title_full_unstemmed | Evaluation of the Selenotranscriptome Expression in Two Hepatocellular Carcinoma Cell Lines |
| title_short | Evaluation of the Selenotranscriptome Expression in Two Hepatocellular Carcinoma Cell Lines |
| title_sort | evaluation of the selenotranscriptome expression in two hepatocellular carcinoma cell lines |
| url | http://dx.doi.org/10.1155/2015/419561 |
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