Bladder Smooth Muscle Cells Differentiation from Dental Pulp Stem Cells: Future Potential for Bladder Tissue Engineering
Dental pulp stem cells (DPSCs) are multipotent cells capable of differentiating into multiple cell lines, thus providing an alternative source of cell for tissue engineering. Smooth muscle cell (SMC) regeneration is a crucial step in tissue engineering of the urinary bladder. It is known that DPSCs...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/6979368 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850170319085502464 |
|---|---|
| author | Bing Song Wenkai Jiang Amr Alraies Qian Liu Vijay Gudla Julia Oni Xiaoqing Wei Alastair Sloan Longxing Ni Meena Agarwal |
| author_facet | Bing Song Wenkai Jiang Amr Alraies Qian Liu Vijay Gudla Julia Oni Xiaoqing Wei Alastair Sloan Longxing Ni Meena Agarwal |
| author_sort | Bing Song |
| collection | DOAJ |
| description | Dental pulp stem cells (DPSCs) are multipotent cells capable of differentiating into multiple cell lines, thus providing an alternative source of cell for tissue engineering. Smooth muscle cell (SMC) regeneration is a crucial step in tissue engineering of the urinary bladder. It is known that DPSCs have the potential to differentiate into a smooth muscle phenotype in vitro with differentiation agents. However, most of these studies are focused on the vascular SMCs. The optimal approaches to induce human DPSCs to differentiate into bladder SMCs are still under investigation. We demonstrate in this study the ability of human DPSCs to differentiate into bladder SMCs in a growth environment containing bladder SMCs-conditioned medium with the addition of the transforming growth factor beta 1 (TGF-β1). After 14 days of exposure to this medium, the gene and protein expression of SMC-specific marker (α-SMA, desmin, and calponin) increased over time. In particular, myosin was present in differentiated cells after 11 days of induction, which indicated that the cells differentiated into the mature SMCs. These data suggested that human DPSCs could be used as an alternative and less invasive source of stem cells for smooth muscle regeneration, a technology that has applications for bladder tissue engineering. |
| format | Article |
| id | doaj-art-a2fa66d1d4f94a49b7d5c1f6d3745b89 |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-a2fa66d1d4f94a49b7d5c1f6d3745b892025-08-20T02:20:30ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/69793686979368Bladder Smooth Muscle Cells Differentiation from Dental Pulp Stem Cells: Future Potential for Bladder Tissue EngineeringBing Song0Wenkai Jiang1Amr Alraies2Qian Liu3Vijay Gudla4Julia Oni5Xiaoqing Wei6Alastair Sloan7Longxing Ni8Meena Agarwal9School of Dentistry, Cardiff Institute of Tissue Engineering and Repair, Cardiff University, Heath Park, Cardiff CF14 4XY, UKSchool of Dentistry, Cardiff Institute of Tissue Engineering and Repair, Cardiff University, Heath Park, Cardiff CF14 4XY, UKSchool of Dentistry, Cardiff Institute of Tissue Engineering and Repair, Cardiff University, Heath Park, Cardiff CF14 4XY, UKSchool of Dentistry, Cardiff Institute of Tissue Engineering and Repair, Cardiff University, Heath Park, Cardiff CF14 4XY, UKDepartment of Urology/Surgery, Cardiff University and University Hospital of Wales, Heath Park, Cardiff CF14 4XW, UKDepartment of Urology/Surgery, Cardiff University and University Hospital of Wales, Heath Park, Cardiff CF14 4XW, UKSchool of Dentistry, Cardiff Institute of Tissue Engineering and Repair, Cardiff University, Heath Park, Cardiff CF14 4XY, UKSchool of Dentistry, Cardiff Institute of Tissue Engineering and Repair, Cardiff University, Heath Park, Cardiff CF14 4XY, UKState Key Laboratory of Military Stomatology, Department of Operative Dentistry & Endodontics, School of Stomatology, Fourth Military Medical University, Shaanxi 710032, ChinaDepartment of Urology/Surgery, Cardiff University and University Hospital of Wales, Heath Park, Cardiff CF14 4XW, UKDental pulp stem cells (DPSCs) are multipotent cells capable of differentiating into multiple cell lines, thus providing an alternative source of cell for tissue engineering. Smooth muscle cell (SMC) regeneration is a crucial step in tissue engineering of the urinary bladder. It is known that DPSCs have the potential to differentiate into a smooth muscle phenotype in vitro with differentiation agents. However, most of these studies are focused on the vascular SMCs. The optimal approaches to induce human DPSCs to differentiate into bladder SMCs are still under investigation. We demonstrate in this study the ability of human DPSCs to differentiate into bladder SMCs in a growth environment containing bladder SMCs-conditioned medium with the addition of the transforming growth factor beta 1 (TGF-β1). After 14 days of exposure to this medium, the gene and protein expression of SMC-specific marker (α-SMA, desmin, and calponin) increased over time. In particular, myosin was present in differentiated cells after 11 days of induction, which indicated that the cells differentiated into the mature SMCs. These data suggested that human DPSCs could be used as an alternative and less invasive source of stem cells for smooth muscle regeneration, a technology that has applications for bladder tissue engineering.http://dx.doi.org/10.1155/2016/6979368 |
| spellingShingle | Bing Song Wenkai Jiang Amr Alraies Qian Liu Vijay Gudla Julia Oni Xiaoqing Wei Alastair Sloan Longxing Ni Meena Agarwal Bladder Smooth Muscle Cells Differentiation from Dental Pulp Stem Cells: Future Potential for Bladder Tissue Engineering Stem Cells International |
| title | Bladder Smooth Muscle Cells Differentiation from Dental Pulp Stem Cells: Future Potential for Bladder Tissue Engineering |
| title_full | Bladder Smooth Muscle Cells Differentiation from Dental Pulp Stem Cells: Future Potential for Bladder Tissue Engineering |
| title_fullStr | Bladder Smooth Muscle Cells Differentiation from Dental Pulp Stem Cells: Future Potential for Bladder Tissue Engineering |
| title_full_unstemmed | Bladder Smooth Muscle Cells Differentiation from Dental Pulp Stem Cells: Future Potential for Bladder Tissue Engineering |
| title_short | Bladder Smooth Muscle Cells Differentiation from Dental Pulp Stem Cells: Future Potential for Bladder Tissue Engineering |
| title_sort | bladder smooth muscle cells differentiation from dental pulp stem cells future potential for bladder tissue engineering |
| url | http://dx.doi.org/10.1155/2016/6979368 |
| work_keys_str_mv | AT bingsong bladdersmoothmusclecellsdifferentiationfromdentalpulpstemcellsfuturepotentialforbladdertissueengineering AT wenkaijiang bladdersmoothmusclecellsdifferentiationfromdentalpulpstemcellsfuturepotentialforbladdertissueengineering AT amralraies bladdersmoothmusclecellsdifferentiationfromdentalpulpstemcellsfuturepotentialforbladdertissueengineering AT qianliu bladdersmoothmusclecellsdifferentiationfromdentalpulpstemcellsfuturepotentialforbladdertissueengineering AT vijaygudla bladdersmoothmusclecellsdifferentiationfromdentalpulpstemcellsfuturepotentialforbladdertissueengineering AT juliaoni bladdersmoothmusclecellsdifferentiationfromdentalpulpstemcellsfuturepotentialforbladdertissueengineering AT xiaoqingwei bladdersmoothmusclecellsdifferentiationfromdentalpulpstemcellsfuturepotentialforbladdertissueengineering AT alastairsloan bladdersmoothmusclecellsdifferentiationfromdentalpulpstemcellsfuturepotentialforbladdertissueengineering AT longxingni bladdersmoothmusclecellsdifferentiationfromdentalpulpstemcellsfuturepotentialforbladdertissueengineering AT meenaagarwal bladdersmoothmusclecellsdifferentiationfromdentalpulpstemcellsfuturepotentialforbladdertissueengineering |