Testosterone levels positively linked to muscle mass but not strength in adult males aged 20–59 years: a cross-sectional study

BackgroundThe relationship between testosterone levels and muscle mass and strength remains contentious. This study aimed to explore the relationship among total serum testosterone levels, muscle mass, and strength in young to middle-aged adults.MethodsThe analysis included 4,495 participants (age 3...

Full description

Saved in:
Bibliographic Details
Main Authors: Wei Zhang, Zhaohui Cui, Dayong Shen, Li Gao, Qingyun Li
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-04-01
Series:Frontiers in Physiology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fphys.2025.1512268/full
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849731606355378176
author Wei Zhang
Zhaohui Cui
Dayong Shen
Li Gao
Qingyun Li
author_facet Wei Zhang
Zhaohui Cui
Dayong Shen
Li Gao
Qingyun Li
author_sort Wei Zhang
collection DOAJ
description BackgroundThe relationship between testosterone levels and muscle mass and strength remains contentious. This study aimed to explore the relationship among total serum testosterone levels, muscle mass, and strength in young to middle-aged adults.MethodsThe analysis included 4,495 participants (age 39.2 ± 0.2 years, mean ± SE) from the National Health and Nutrition Examination Survey conducted between 2011 and 2014. Weighted regression models were used to assess the association of testosterone levels with muscle mass and strength.ResultsFor male participants, log2-transformed testosterone levels were positively associated with appendicular lean mass adjusted for body mass index (β: 0.05, 95% confidence interval [CI]: 0.03–0.07, P < 0.001) and negatively associated with low muscle mass (odds ratio: 0.40, 95% CI: 0.24–0.67, P = 0.006). However, no significant association was found between testosterone levels and grip strength (β: 1.16, 95% CI: 0.26 to 2.58, P = 0.086) or low muscle strength (odds ratio: 0.51, 95% CI: 0.25–1.04, P = 0.059). For female participants, no significant association was observed between testosterone levels and muscle mass (β: 0.01, 95% CI: 0.02 to −0.01, P = 0.294) or muscle strength (β: 0.14, 95% CI: 0.45 to 0.73, P = 0.508). Restricted cubic spline analysis revealed a linear relationship between total testosterone levels and appendicular lean mass adjusted for body mass index in male participants (nonlinear: P = 0.367).ConclusionOur study indicates that testosterone levels are positively associated with muscle mass but not with muscle strength in young to middle-aged males.
format Article
id doaj-art-a2ed0df435f34d3eaba644386c57fd44
institution DOAJ
issn 1664-042X
language English
publishDate 2025-04-01
publisher Frontiers Media S.A.
record_format Article
series Frontiers in Physiology
spelling doaj-art-a2ed0df435f34d3eaba644386c57fd442025-08-20T03:08:29ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2025-04-011610.3389/fphys.2025.15122681512268Testosterone levels positively linked to muscle mass but not strength in adult males aged 20–59 years: a cross-sectional studyWei Zhang0Zhaohui Cui1Dayong Shen2Li Gao3Qingyun Li4Department of Neurology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, ChinaDepartment of Orthopedic Surgery, Heze Municipal Hospital, Heze, ChinaDepartment of Neurology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, ChinaDepartment of Endocrinology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, ChinaDepartment of Neurology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, ChinaBackgroundThe relationship between testosterone levels and muscle mass and strength remains contentious. This study aimed to explore the relationship among total serum testosterone levels, muscle mass, and strength in young to middle-aged adults.MethodsThe analysis included 4,495 participants (age 39.2 ± 0.2 years, mean ± SE) from the National Health and Nutrition Examination Survey conducted between 2011 and 2014. Weighted regression models were used to assess the association of testosterone levels with muscle mass and strength.ResultsFor male participants, log2-transformed testosterone levels were positively associated with appendicular lean mass adjusted for body mass index (β: 0.05, 95% confidence interval [CI]: 0.03–0.07, P < 0.001) and negatively associated with low muscle mass (odds ratio: 0.40, 95% CI: 0.24–0.67, P = 0.006). However, no significant association was found between testosterone levels and grip strength (β: 1.16, 95% CI: 0.26 to 2.58, P = 0.086) or low muscle strength (odds ratio: 0.51, 95% CI: 0.25–1.04, P = 0.059). For female participants, no significant association was observed between testosterone levels and muscle mass (β: 0.01, 95% CI: 0.02 to −0.01, P = 0.294) or muscle strength (β: 0.14, 95% CI: 0.45 to 0.73, P = 0.508). Restricted cubic spline analysis revealed a linear relationship between total testosterone levels and appendicular lean mass adjusted for body mass index in male participants (nonlinear: P = 0.367).ConclusionOur study indicates that testosterone levels are positively associated with muscle mass but not with muscle strength in young to middle-aged males.https://www.frontiersin.org/articles/10.3389/fphys.2025.1512268/fullsex differencestestosteronemuscle massmuscle strengthNHANES
spellingShingle Wei Zhang
Zhaohui Cui
Dayong Shen
Li Gao
Qingyun Li
Testosterone levels positively linked to muscle mass but not strength in adult males aged 20–59 years: a cross-sectional study
Frontiers in Physiology
sex differences
testosterone
muscle mass
muscle strength
NHANES
title Testosterone levels positively linked to muscle mass but not strength in adult males aged 20–59 years: a cross-sectional study
title_full Testosterone levels positively linked to muscle mass but not strength in adult males aged 20–59 years: a cross-sectional study
title_fullStr Testosterone levels positively linked to muscle mass but not strength in adult males aged 20–59 years: a cross-sectional study
title_full_unstemmed Testosterone levels positively linked to muscle mass but not strength in adult males aged 20–59 years: a cross-sectional study
title_short Testosterone levels positively linked to muscle mass but not strength in adult males aged 20–59 years: a cross-sectional study
title_sort testosterone levels positively linked to muscle mass but not strength in adult males aged 20 59 years a cross sectional study
topic sex differences
testosterone
muscle mass
muscle strength
NHANES
url https://www.frontiersin.org/articles/10.3389/fphys.2025.1512268/full
work_keys_str_mv AT weizhang testosteronelevelspositivelylinkedtomusclemassbutnotstrengthinadultmalesaged2059yearsacrosssectionalstudy
AT zhaohuicui testosteronelevelspositivelylinkedtomusclemassbutnotstrengthinadultmalesaged2059yearsacrosssectionalstudy
AT dayongshen testosteronelevelspositivelylinkedtomusclemassbutnotstrengthinadultmalesaged2059yearsacrosssectionalstudy
AT ligao testosteronelevelspositivelylinkedtomusclemassbutnotstrengthinadultmalesaged2059yearsacrosssectionalstudy
AT qingyunli testosteronelevelspositivelylinkedtomusclemassbutnotstrengthinadultmalesaged2059yearsacrosssectionalstudy