Therapeutic Effects of Momordica charantia L. Ethanolic Extract on Acetic Acid-Induced Ulcerative Colitis in Rats
Objective: This study aims to investigate the effect of Momordica charantia L. (MoC) ethanolic extract on ulcerative colitis (UC) and was explored in vitro and in vivo. Materials and Methods: The rats were divided into control (C), saline-treated colitis (AA), MoC extract-treated colitis (AA+MoC), a...
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Istanbul University Press
2021-12-01
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| Series: | European Journal of Biology |
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| Online Access: | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/F2099924AC284EEDB455A60C234F56D0 |
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| author | Dilek Özbeyli Ali Şen Aslı Aykaç Kerem Teralı Özlem Tuğçe Çilingir Kaya İsmail Şenkardeş Göksel Şener |
| author_facet | Dilek Özbeyli Ali Şen Aslı Aykaç Kerem Teralı Özlem Tuğçe Çilingir Kaya İsmail Şenkardeş Göksel Şener |
| author_sort | Dilek Özbeyli |
| collection | DOAJ |
| description | Objective: This study aims to investigate the effect of Momordica charantia L. (MoC) ethanolic extract on ulcerative colitis (UC) and was explored in vitro and in vivo. Materials and Methods: The rats were divided into control (C), saline-treated colitis (AA), MoC extract-treated colitis (AA+MoC), and sulfasalazine (SS)-treated colitis (AA+SS) groups. Colitis was induced by acetic acid. MoC extract, SS or saline were given to the related groups for 3 days. Interleukine-1β, malondialdehyde, glutathione levels, myeloperoxidase activity, bax/bcl-2 ratio, caspase-9 and caspase-3 levels were measured in colon and macroscopic and histopathologic examinations were done. Total phenolic/flavonoid content and biological activity of MoC were evaluated by in vitro analysis. Results: Compared to the control group, with acetic acid application interleukin-1β levels, myeloperoxidase activity, malondialdehyde levels, bax/bcl-2 ratio, caspase-9 and caspase-3 levels were significantly upregulated, while glutathione levels were significantly decreased in the AA group. In contrast, MoC and SS treatments reduced interleukin-1β, malondialdehyde levels, myeloperoxidase activity, bax/bcl-2 ratio, and caspase-9 and caspase-3 levels. Glutathione levels increased upon MoC or SS treatment. Increased macroscopic and microscopic scoring with AA improved with MoC or SS treatment, but the MoC or SS treated groups had higher score values than the control. Also, in vitro results showed that MoC exhibited 2,2-diphenyl-1- picrylhydrazyl and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid radical scavenging activity as well as significant antilipoxygenase activity. In addition, MoC extract showed a potent anti-inflammatory activity compared to standard indomethacin. Conclusion: Our biochemical, in vitro and histopathologic analysis indicate that MoC is likely to prove beneficial in UC therapy. |
| format | Article |
| id | doaj-art-a2eb6faa3cd14292b1d7f0251c198d4d |
| institution | OA Journals |
| issn | 2618-6144 |
| language | English |
| publishDate | 2021-12-01 |
| publisher | Istanbul University Press |
| record_format | Article |
| series | European Journal of Biology |
| spelling | doaj-art-a2eb6faa3cd14292b1d7f0251c198d4d2025-08-20T02:10:56ZengIstanbul University PressEuropean Journal of Biology2618-61442021-12-0180211912810.26650/EurJBiol.2021.1016222123456Therapeutic Effects of Momordica charantia L. Ethanolic Extract on Acetic Acid-Induced Ulcerative Colitis in RatsDilek Özbeyli0https://orcid.org/0000-0002-4141-6913Ali Şen1https://orcid.org/0000-0002-2144-5741Aslı Aykaç2https://orcid.org/0000-0002-4885-5070Kerem Teralı3https://orcid.org/0000-0002-9964-6383Özlem Tuğçe Çilingir Kaya4https://orcid.org/0000-0002-2591-9174İsmail Şenkardeş5https://orcid.org/0000-0002-2656-0319Göksel Şener6https://orcid.org/0000-0001-7444-6193Marmara Üniversitesi, İstanbul, TürkiyeMarmara Üniversitesi, İstanbul, TürkiyeYakın Doğu Üniversitesi, Nicosia, CyprusGirne American University, Kyrenia, CyprusMarmara Üniversitesi, İstanbul, TürkiyeMarmara Üniversitesi, İstanbul, TürkiyeFenerbahçe Üniversitesi, Istanbul, TurkiyeObjective: This study aims to investigate the effect of Momordica charantia L. (MoC) ethanolic extract on ulcerative colitis (UC) and was explored in vitro and in vivo. Materials and Methods: The rats were divided into control (C), saline-treated colitis (AA), MoC extract-treated colitis (AA+MoC), and sulfasalazine (SS)-treated colitis (AA+SS) groups. Colitis was induced by acetic acid. MoC extract, SS or saline were given to the related groups for 3 days. Interleukine-1β, malondialdehyde, glutathione levels, myeloperoxidase activity, bax/bcl-2 ratio, caspase-9 and caspase-3 levels were measured in colon and macroscopic and histopathologic examinations were done. Total phenolic/flavonoid content and biological activity of MoC were evaluated by in vitro analysis. Results: Compared to the control group, with acetic acid application interleukin-1β levels, myeloperoxidase activity, malondialdehyde levels, bax/bcl-2 ratio, caspase-9 and caspase-3 levels were significantly upregulated, while glutathione levels were significantly decreased in the AA group. In contrast, MoC and SS treatments reduced interleukin-1β, malondialdehyde levels, myeloperoxidase activity, bax/bcl-2 ratio, and caspase-9 and caspase-3 levels. Glutathione levels increased upon MoC or SS treatment. Increased macroscopic and microscopic scoring with AA improved with MoC or SS treatment, but the MoC or SS treated groups had higher score values than the control. Also, in vitro results showed that MoC exhibited 2,2-diphenyl-1- picrylhydrazyl and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid radical scavenging activity as well as significant antilipoxygenase activity. In addition, MoC extract showed a potent anti-inflammatory activity compared to standard indomethacin. Conclusion: Our biochemical, in vitro and histopathologic analysis indicate that MoC is likely to prove beneficial in UC therapy.https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/F2099924AC284EEDB455A60C234F56D0momordica charantia l.ulcerative colitisapoptosisoxidative stressradical scavenging activityanti-lipoxygenase activity |
| spellingShingle | Dilek Özbeyli Ali Şen Aslı Aykaç Kerem Teralı Özlem Tuğçe Çilingir Kaya İsmail Şenkardeş Göksel Şener Therapeutic Effects of Momordica charantia L. Ethanolic Extract on Acetic Acid-Induced Ulcerative Colitis in Rats European Journal of Biology momordica charantia l. ulcerative colitis apoptosis oxidative stress radical scavenging activity anti-lipoxygenase activity |
| title | Therapeutic Effects of Momordica charantia L. Ethanolic Extract on Acetic Acid-Induced Ulcerative Colitis in Rats |
| title_full | Therapeutic Effects of Momordica charantia L. Ethanolic Extract on Acetic Acid-Induced Ulcerative Colitis in Rats |
| title_fullStr | Therapeutic Effects of Momordica charantia L. Ethanolic Extract on Acetic Acid-Induced Ulcerative Colitis in Rats |
| title_full_unstemmed | Therapeutic Effects of Momordica charantia L. Ethanolic Extract on Acetic Acid-Induced Ulcerative Colitis in Rats |
| title_short | Therapeutic Effects of Momordica charantia L. Ethanolic Extract on Acetic Acid-Induced Ulcerative Colitis in Rats |
| title_sort | therapeutic effects of momordica charantia l ethanolic extract on acetic acid induced ulcerative colitis in rats |
| topic | momordica charantia l. ulcerative colitis apoptosis oxidative stress radical scavenging activity anti-lipoxygenase activity |
| url | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/F2099924AC284EEDB455A60C234F56D0 |
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