Aluminum phthalocyanine nanoparticles activation for local fluorescence spectroscopy in dentistry
Early diagnosis of caries and tooth enamel microcracks is of great importance for preventing the destruction of healthy tooth enamel. Inorder to detect microcracks in the enamel and pathogenic microflora foci that can cause caries, nanoform of aluminum phthalocyanine (AlPc) can be used as a marker....
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| Language: | Russian |
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Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis "Russian Photodynamic Association"
2018-10-01
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| Series: | Biomedical Photonics |
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| Online Access: | https://www.pdt-journal.com/jour/article/view/248 |
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| author | J. O. Zolotareva D. S. Farrakhova E. N. Kupriyanova V. B. Loschenov |
| author_facet | J. O. Zolotareva D. S. Farrakhova E. N. Kupriyanova V. B. Loschenov |
| author_sort | J. O. Zolotareva |
| collection | DOAJ |
| description | Early diagnosis of caries and tooth enamel microcracks is of great importance for preventing the destruction of healthy tooth enamel. Inorder to detect microcracks in the enamel and pathogenic microflora foci that can cause caries, nanoform of aluminum phthalocyanine (AlPc) can be used as a marker. In a colloidal solution, the nanoparticles do not fluoresce, unlike their molecular form. To convert the particle into its molecular form, it is necessary to have a solvent or specific environment (bacteria, macrophages, etc.). That is why the hydrophobic nanoparticles of aluminum phthalocyanine (nAlPc) can act as markers for detecting hidden pathogenic microflora during fluorescent diagnostics. Further reduction of the diagnosis time and increase the efficiency can be achieved by using biologically compatible surfactants as additional activators of nAlPc.In order to carry out local fluorescence spectroscopy of enamel microcracks and pathogenic microflora foci on the enamel surface, a model compound containing surfactants, auxiliary components and nAlPc colloid at a concentration of 10 mg/l was prepared.Studies on the interaction of the model compound with nAlPc and Protelan MST-35 with tooth enamel ex vivo have shown this surfactant to be a promising auxiliary activator of the nanoparticles, allowing conducting local fluorescence spectroscopy of the tooth enamel surface 3 min after application. In addition, statistical processing of the results showed the effectiveness of using the model compound for local fluorescence spectroscopy of the enamel surface in order to detect the enamel microcracks and the pathogenic microflora accumulation foci that can lead to the development of a cariogenic process. |
| format | Article |
| id | doaj-art-a2e2528e1be34673ab642990f45219d2 |
| institution | Kabale University |
| issn | 2413-9432 |
| language | Russian |
| publishDate | 2018-10-01 |
| publisher | Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis "Russian Photodynamic Association" |
| record_format | Article |
| series | Biomedical Photonics |
| spelling | doaj-art-a2e2528e1be34673ab642990f45219d22025-08-20T04:00:13ZrusNon-profit partnership for development of domestic photodynamic therapy and photodiagnosis "Russian Photodynamic Association"Biomedical Photonics2413-94322018-10-017342010.24931/2413-9432-2018-7-3-4-20143Aluminum phthalocyanine nanoparticles activation for local fluorescence spectroscopy in dentistryJ. O. Zolotareva0D. S. Farrakhova1E. N. Kupriyanova2V. B. Loschenov3National Research Nuclear University MEPhINational Research Nuclear University MEPhI; Prokhorov General Physics Institute of the Russian Academy of Sciences; Dentospek LLCSAHI «Dental Clinic No. 11 of the Moscow City Health Department»National Research Nuclear University MEPhI; Prokhorov General Physics Institute of the Russian Academy of Sciences; Dentospek LLCEarly diagnosis of caries and tooth enamel microcracks is of great importance for preventing the destruction of healthy tooth enamel. Inorder to detect microcracks in the enamel and pathogenic microflora foci that can cause caries, nanoform of aluminum phthalocyanine (AlPc) can be used as a marker. In a colloidal solution, the nanoparticles do not fluoresce, unlike their molecular form. To convert the particle into its molecular form, it is necessary to have a solvent or specific environment (bacteria, macrophages, etc.). That is why the hydrophobic nanoparticles of aluminum phthalocyanine (nAlPc) can act as markers for detecting hidden pathogenic microflora during fluorescent diagnostics. Further reduction of the diagnosis time and increase the efficiency can be achieved by using biologically compatible surfactants as additional activators of nAlPc.In order to carry out local fluorescence spectroscopy of enamel microcracks and pathogenic microflora foci on the enamel surface, a model compound containing surfactants, auxiliary components and nAlPc colloid at a concentration of 10 mg/l was prepared.Studies on the interaction of the model compound with nAlPc and Protelan MST-35 with tooth enamel ex vivo have shown this surfactant to be a promising auxiliary activator of the nanoparticles, allowing conducting local fluorescence spectroscopy of the tooth enamel surface 3 min after application. In addition, statistical processing of the results showed the effectiveness of using the model compound for local fluorescence spectroscopy of the enamel surface in order to detect the enamel microcracks and the pathogenic microflora accumulation foci that can lead to the development of a cariogenic process.https://www.pdt-journal.com/jour/article/view/248local fluorescence spectroscopynanoparticlesaluminum phthalocyaninefluorescenceenamel microcrackscaries |
| spellingShingle | J. O. Zolotareva D. S. Farrakhova E. N. Kupriyanova V. B. Loschenov Aluminum phthalocyanine nanoparticles activation for local fluorescence spectroscopy in dentistry Biomedical Photonics local fluorescence spectroscopy nanoparticles aluminum phthalocyanine fluorescence enamel microcracks caries |
| title | Aluminum phthalocyanine nanoparticles activation for local fluorescence spectroscopy in dentistry |
| title_full | Aluminum phthalocyanine nanoparticles activation for local fluorescence spectroscopy in dentistry |
| title_fullStr | Aluminum phthalocyanine nanoparticles activation for local fluorescence spectroscopy in dentistry |
| title_full_unstemmed | Aluminum phthalocyanine nanoparticles activation for local fluorescence spectroscopy in dentistry |
| title_short | Aluminum phthalocyanine nanoparticles activation for local fluorescence spectroscopy in dentistry |
| title_sort | aluminum phthalocyanine nanoparticles activation for local fluorescence spectroscopy in dentistry |
| topic | local fluorescence spectroscopy nanoparticles aluminum phthalocyanine fluorescence enamel microcracks caries |
| url | https://www.pdt-journal.com/jour/article/view/248 |
| work_keys_str_mv | AT jozolotareva aluminumphthalocyaninenanoparticlesactivationforlocalfluorescencespectroscopyindentistry AT dsfarrakhova aluminumphthalocyaninenanoparticlesactivationforlocalfluorescencespectroscopyindentistry AT enkupriyanova aluminumphthalocyaninenanoparticlesactivationforlocalfluorescencespectroscopyindentistry AT vbloschenov aluminumphthalocyaninenanoparticlesactivationforlocalfluorescencespectroscopyindentistry |