Aspects of Electrochemical Biosensors Using Affinity Assays
In recent decades, the utilization of biomarkers has gained increasing attention. The timely identification and quantification of proteins, nucleic acids, and small molecules associated with a medical condition, infection, or contaminant have become increasingly crucial across a variety of fields, i...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-03-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/15/3/166 |
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| author | Thor Pedersen Leonid Gurevich Nils E. Magnusson |
| author_facet | Thor Pedersen Leonid Gurevich Nils E. Magnusson |
| author_sort | Thor Pedersen |
| collection | DOAJ |
| description | In recent decades, the utilization of biomarkers has gained increasing attention. The timely identification and quantification of proteins, nucleic acids, and small molecules associated with a medical condition, infection, or contaminant have become increasingly crucial across a variety of fields, including medicine, food safety, and quality/environmental control. State-of-the-art biomarker detection methods predominantly rely on standard immunoassay techniques, requiring specialized laboratory equipment and trained personnel. This impedes the broad commercial implementation of biosensors in, e.g., Point-of-Care (PoC) settings where ease of operation, portability, and cost-efficiency are prioritized. Small, robust electrochemical biosensors are a promising alternative for analyzing biomarkers in complex samples within PoC environments. Therefore, creating and designing optimized sensing surfaces, immobilization strategies, and efficient signal generation are crucial for improving biosensor systems, which in turn can have real-world impact. In the present paper, we reviewed common electrode types and geometries used in electrochemical biosensors and the immobilization approaches, discussed the advantages and drawbacks of different electrochemical detection methods, and presented different labeling strategies for signal generation and enhancement. |
| format | Article |
| id | doaj-art-a2dbfe16e2d745d690dfedad61d04e00 |
| institution | DOAJ |
| issn | 2079-6374 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-a2dbfe16e2d745d690dfedad61d04e002025-08-20T02:42:42ZengMDPI AGBiosensors2079-63742025-03-0115316610.3390/bios15030166Aspects of Electrochemical Biosensors Using Affinity AssaysThor Pedersen0Leonid Gurevich1Nils E. Magnusson2Department of Materials and Production, Aalborg University, Fibigerstræde 16, 9220 Aalborg, DenmarkDepartment of Materials and Production, Aalborg University, Fibigerstræde 16, 9220 Aalborg, DenmarkBiostrip APS, Lindevangsvej 10, 8240 Risskov, DenmarkIn recent decades, the utilization of biomarkers has gained increasing attention. The timely identification and quantification of proteins, nucleic acids, and small molecules associated with a medical condition, infection, or contaminant have become increasingly crucial across a variety of fields, including medicine, food safety, and quality/environmental control. State-of-the-art biomarker detection methods predominantly rely on standard immunoassay techniques, requiring specialized laboratory equipment and trained personnel. This impedes the broad commercial implementation of biosensors in, e.g., Point-of-Care (PoC) settings where ease of operation, portability, and cost-efficiency are prioritized. Small, robust electrochemical biosensors are a promising alternative for analyzing biomarkers in complex samples within PoC environments. Therefore, creating and designing optimized sensing surfaces, immobilization strategies, and efficient signal generation are crucial for improving biosensor systems, which in turn can have real-world impact. In the present paper, we reviewed common electrode types and geometries used in electrochemical biosensors and the immobilization approaches, discussed the advantages and drawbacks of different electrochemical detection methods, and presented different labeling strategies for signal generation and enhancement.https://www.mdpi.com/2079-6374/15/3/166biosensornanomaterialselectrochemistryscreen-printed electrodeselectrochemical labelselectrochemical biosensor |
| spellingShingle | Thor Pedersen Leonid Gurevich Nils E. Magnusson Aspects of Electrochemical Biosensors Using Affinity Assays Biosensors biosensor nanomaterials electrochemistry screen-printed electrodes electrochemical labels electrochemical biosensor |
| title | Aspects of Electrochemical Biosensors Using Affinity Assays |
| title_full | Aspects of Electrochemical Biosensors Using Affinity Assays |
| title_fullStr | Aspects of Electrochemical Biosensors Using Affinity Assays |
| title_full_unstemmed | Aspects of Electrochemical Biosensors Using Affinity Assays |
| title_short | Aspects of Electrochemical Biosensors Using Affinity Assays |
| title_sort | aspects of electrochemical biosensors using affinity assays |
| topic | biosensor nanomaterials electrochemistry screen-printed electrodes electrochemical labels electrochemical biosensor |
| url | https://www.mdpi.com/2079-6374/15/3/166 |
| work_keys_str_mv | AT thorpedersen aspectsofelectrochemicalbiosensorsusingaffinityassays AT leonidgurevich aspectsofelectrochemicalbiosensorsusingaffinityassays AT nilsemagnusson aspectsofelectrochemicalbiosensorsusingaffinityassays |