Computational design of binding proteins to EGFR domain II.
We developed a process to produce novel interactions between two previously unrelated proteins. This process selects protein scaffolds and designs protein interfaces that bind to a surface patch of interest on a target protein. Scaffolds with shapes complementary to the target surface patch were scr...
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| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0092513&type=printable |
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| _version_ | 1850191438800748544 |
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| author | Yoon Sup Choi Soomin Yoon Kyung-Lock Kim Jiho Yoo Parkyong Song Minsoo Kim Young-Eun Shin Won Jun Yang Jung-eun Noh Hyun-Soo Cho Sanguk Kim Junho Chung Sung Ho Ryu |
| author_facet | Yoon Sup Choi Soomin Yoon Kyung-Lock Kim Jiho Yoo Parkyong Song Minsoo Kim Young-Eun Shin Won Jun Yang Jung-eun Noh Hyun-Soo Cho Sanguk Kim Junho Chung Sung Ho Ryu |
| author_sort | Yoon Sup Choi |
| collection | DOAJ |
| description | We developed a process to produce novel interactions between two previously unrelated proteins. This process selects protein scaffolds and designs protein interfaces that bind to a surface patch of interest on a target protein. Scaffolds with shapes complementary to the target surface patch were screened using an exhaustive computational search of the human proteome and optimized by directed evolution using phage display. This method was applied to successfully design scaffolds that bind to epidermal growth factor receptor (EGFR) domain II, the interface of EGFR dimerization, with high reactivity toward the target surface patch of EGFR domain II. One potential application of these tailor-made protein interactions is the development of therapeutic agents against specific protein targets. |
| format | Article |
| id | doaj-art-a2d3722fb48247248b0db2e254f5bea7 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-a2d3722fb48247248b0db2e254f5bea72025-08-20T02:14:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9251310.1371/journal.pone.0092513Computational design of binding proteins to EGFR domain II.Yoon Sup ChoiSoomin YoonKyung-Lock KimJiho YooParkyong SongMinsoo KimYoung-Eun ShinWon Jun YangJung-eun NohHyun-Soo ChoSanguk KimJunho ChungSung Ho RyuWe developed a process to produce novel interactions between two previously unrelated proteins. This process selects protein scaffolds and designs protein interfaces that bind to a surface patch of interest on a target protein. Scaffolds with shapes complementary to the target surface patch were screened using an exhaustive computational search of the human proteome and optimized by directed evolution using phage display. This method was applied to successfully design scaffolds that bind to epidermal growth factor receptor (EGFR) domain II, the interface of EGFR dimerization, with high reactivity toward the target surface patch of EGFR domain II. One potential application of these tailor-made protein interactions is the development of therapeutic agents against specific protein targets.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0092513&type=printable |
| spellingShingle | Yoon Sup Choi Soomin Yoon Kyung-Lock Kim Jiho Yoo Parkyong Song Minsoo Kim Young-Eun Shin Won Jun Yang Jung-eun Noh Hyun-Soo Cho Sanguk Kim Junho Chung Sung Ho Ryu Computational design of binding proteins to EGFR domain II. PLoS ONE |
| title | Computational design of binding proteins to EGFR domain II. |
| title_full | Computational design of binding proteins to EGFR domain II. |
| title_fullStr | Computational design of binding proteins to EGFR domain II. |
| title_full_unstemmed | Computational design of binding proteins to EGFR domain II. |
| title_short | Computational design of binding proteins to EGFR domain II. |
| title_sort | computational design of binding proteins to egfr domain ii |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0092513&type=printable |
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