Genetically engineered secretory horseradish peroxidase is a sensitive, stable, and affordable non-lytic reporter gene system for real-time promoter activity management
Abstract A light-producing secretory protein that is collectible through the supernatant of a culture medium is essential in a cell-based reporter gene system and allows for real-time monitoring of upstream events of a promoter. Compared to other secretory luciferases, Cypridina luciferase (CLuc) co...
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| Main Authors: | Mu-Shen Chang, Chia-Yi Lee, Yu-Yen Chang, Pin-Jie Li, Hsin-Yu Wu, En-Shuo Liu, Hsin-Kai Huang, Wen-Wei Lin |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-04-01
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| Series: | Journal of Biological Engineering |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13036-025-00508-w |
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