The Functional Network of PrkC and Its Interaction Proteins in <i>Bacillus subtilis</i> Spores
In the food industry, food spoilage caused by spores is a pressing scientific challenge that needs to be addressed urgently, and spore germination is a key approach to solving this problem. Studies have shown that peptidoglycan-induced spore germination represents a novel mechanism of action, which...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-03-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/13/4/744 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850180341915975680 |
|---|---|
| author | Kangyi Mu Tianlin Cui Zequn Zhang Yicong Shi Chen Fang Li Dong Xiaosong Hu |
| author_facet | Kangyi Mu Tianlin Cui Zequn Zhang Yicong Shi Chen Fang Li Dong Xiaosong Hu |
| author_sort | Kangyi Mu |
| collection | DOAJ |
| description | In the food industry, food spoilage caused by spores is a pressing scientific challenge that needs to be addressed urgently, and spore germination is a key approach to solving this problem. Studies have shown that peptidoglycan-induced spore germination represents a novel mechanism of action, which can bind to the PASTA domain of the serine/threonine kinase PrkC. However, the signaling mechanism of peptidoglycan-induced spore germination remains unclear. This study focuses on <i>Bacillus subtilis</i>, using pull-down experiments to screen for proteins interacting with PrkC. There are 80 interaction proteins of PrkC that were identified in the spore. GO analysis reveals that PrkC-interacting proteins in the spore are mainly involved in metabolic processes, cell part and catalysis. KEGG results indicate that PrkC-interacting proteins in the spore are mainly involved in RNA degradation, quorum sensing, oxidative phosphorylation, etc. Additionally, proteins are categorized into six groups by function based on events that may be associated with post-germination triggered by peptidoglycan-induced activation of the PrkC signaling pathway, including “stimulate translation initiation” and “ATP synthesis and energy metabolism”. The experimental results provide a theoretical basis for further elucidating the signaling mechanism of PrkC, revealing the signaling pathway of peptidoglycan-induced spore germination, and identifying targeted inducers and repressors of spore germination. |
| format | Article |
| id | doaj-art-a2acffa69abb45048e3f4981ac6edbf3 |
| institution | OA Journals |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-a2acffa69abb45048e3f4981ac6edbf32025-08-20T02:18:14ZengMDPI AGMicroorganisms2076-26072025-03-0113474410.3390/microorganisms13040744The Functional Network of PrkC and Its Interaction Proteins in <i>Bacillus subtilis</i> SporesKangyi Mu0Tianlin Cui1Zequn Zhang2Yicong Shi3Chen Fang4Li Dong5Xiaosong Hu6Key Laboratory of Fruits and Vegetables Processing, Ministry of Agriculture, Engineering Research Centre for Fruits and Vegetables Processing, Ministry of Education, National, Engineering Research Center for Fruit and Vegetable Processing, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaKey Laboratory of Fruits and Vegetables Processing, Ministry of Agriculture, Engineering Research Centre for Fruits and Vegetables Processing, Ministry of Education, National, Engineering Research Center for Fruit and Vegetable Processing, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, ChinaKey Laboratory of Fruits and Vegetables Processing, Ministry of Agriculture, Engineering Research Centre for Fruits and Vegetables Processing, Ministry of Education, National, Engineering Research Center for Fruit and Vegetable Processing, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaKey Laboratory of Fruits and Vegetables Processing, Ministry of Agriculture, Engineering Research Centre for Fruits and Vegetables Processing, Ministry of Education, National, Engineering Research Center for Fruit and Vegetable Processing, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaKey Laboratory of Fruits and Vegetables Processing, Ministry of Agriculture, Engineering Research Centre for Fruits and Vegetables Processing, Ministry of Education, National, Engineering Research Center for Fruit and Vegetable Processing, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaKey Laboratory of Fruits and Vegetables Processing, Ministry of Agriculture, Engineering Research Centre for Fruits and Vegetables Processing, Ministry of Education, National, Engineering Research Center for Fruit and Vegetable Processing, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaIn the food industry, food spoilage caused by spores is a pressing scientific challenge that needs to be addressed urgently, and spore germination is a key approach to solving this problem. Studies have shown that peptidoglycan-induced spore germination represents a novel mechanism of action, which can bind to the PASTA domain of the serine/threonine kinase PrkC. However, the signaling mechanism of peptidoglycan-induced spore germination remains unclear. This study focuses on <i>Bacillus subtilis</i>, using pull-down experiments to screen for proteins interacting with PrkC. There are 80 interaction proteins of PrkC that were identified in the spore. GO analysis reveals that PrkC-interacting proteins in the spore are mainly involved in metabolic processes, cell part and catalysis. KEGG results indicate that PrkC-interacting proteins in the spore are mainly involved in RNA degradation, quorum sensing, oxidative phosphorylation, etc. Additionally, proteins are categorized into six groups by function based on events that may be associated with post-germination triggered by peptidoglycan-induced activation of the PrkC signaling pathway, including “stimulate translation initiation” and “ATP synthesis and energy metabolism”. The experimental results provide a theoretical basis for further elucidating the signaling mechanism of PrkC, revealing the signaling pathway of peptidoglycan-induced spore germination, and identifying targeted inducers and repressors of spore germination.https://www.mdpi.com/2076-2607/13/4/744<i>Bacillus subtilis</i>spore germinationPrkCinteraction protein |
| spellingShingle | Kangyi Mu Tianlin Cui Zequn Zhang Yicong Shi Chen Fang Li Dong Xiaosong Hu The Functional Network of PrkC and Its Interaction Proteins in <i>Bacillus subtilis</i> Spores Microorganisms <i>Bacillus subtilis</i> spore germination PrkC interaction protein |
| title | The Functional Network of PrkC and Its Interaction Proteins in <i>Bacillus subtilis</i> Spores |
| title_full | The Functional Network of PrkC and Its Interaction Proteins in <i>Bacillus subtilis</i> Spores |
| title_fullStr | The Functional Network of PrkC and Its Interaction Proteins in <i>Bacillus subtilis</i> Spores |
| title_full_unstemmed | The Functional Network of PrkC and Its Interaction Proteins in <i>Bacillus subtilis</i> Spores |
| title_short | The Functional Network of PrkC and Its Interaction Proteins in <i>Bacillus subtilis</i> Spores |
| title_sort | functional network of prkc and its interaction proteins in i bacillus subtilis i spores |
| topic | <i>Bacillus subtilis</i> spore germination PrkC interaction protein |
| url | https://www.mdpi.com/2076-2607/13/4/744 |
| work_keys_str_mv | AT kangyimu thefunctionalnetworkofprkcanditsinteractionproteinsinibacillussubtilisispores AT tianlincui thefunctionalnetworkofprkcanditsinteractionproteinsinibacillussubtilisispores AT zequnzhang thefunctionalnetworkofprkcanditsinteractionproteinsinibacillussubtilisispores AT yicongshi thefunctionalnetworkofprkcanditsinteractionproteinsinibacillussubtilisispores AT chenfang thefunctionalnetworkofprkcanditsinteractionproteinsinibacillussubtilisispores AT lidong thefunctionalnetworkofprkcanditsinteractionproteinsinibacillussubtilisispores AT xiaosonghu thefunctionalnetworkofprkcanditsinteractionproteinsinibacillussubtilisispores AT kangyimu functionalnetworkofprkcanditsinteractionproteinsinibacillussubtilisispores AT tianlincui functionalnetworkofprkcanditsinteractionproteinsinibacillussubtilisispores AT zequnzhang functionalnetworkofprkcanditsinteractionproteinsinibacillussubtilisispores AT yicongshi functionalnetworkofprkcanditsinteractionproteinsinibacillussubtilisispores AT chenfang functionalnetworkofprkcanditsinteractionproteinsinibacillussubtilisispores AT lidong functionalnetworkofprkcanditsinteractionproteinsinibacillussubtilisispores AT xiaosonghu functionalnetworkofprkcanditsinteractionproteinsinibacillussubtilisispores |