Oxyresveratrol enhances hair regeneration in human dermal papilla cell and androgenetic alopecia mouse model

Abstract Alopecia, or hair loss, is a common dermatological condition caused by multiple factors. Oxyresveratrol (ORV), a compound derived from the heartwood of Artocarpus lakoocha, is recognized for its potent antioxidant properties, with recent studies highlighting its anti-inflammatory effect acr...

Full description

Saved in:
Bibliographic Details
Main Authors: Hung Gia Tran, Aussavashai Shuayprom, Alisa Ruchusatsawat, Kornvalee Meesilpavikkai, Virgil A. S. H. Dalm, Kriangsak Ruchusatsawat, Jongkonnee Wongpiyabovorn
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Scientific Reports
Subjects:
Online Access:https://doi.org/10.1038/s41598-025-02581-z
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850269218450178048
author Hung Gia Tran
Aussavashai Shuayprom
Alisa Ruchusatsawat
Kornvalee Meesilpavikkai
Virgil A. S. H. Dalm
Kriangsak Ruchusatsawat
Jongkonnee Wongpiyabovorn
author_facet Hung Gia Tran
Aussavashai Shuayprom
Alisa Ruchusatsawat
Kornvalee Meesilpavikkai
Virgil A. S. H. Dalm
Kriangsak Ruchusatsawat
Jongkonnee Wongpiyabovorn
author_sort Hung Gia Tran
collection DOAJ
description Abstract Alopecia, or hair loss, is a common dermatological condition caused by multiple factors. Oxyresveratrol (ORV), a compound derived from the heartwood of Artocarpus lakoocha, is recognized for its potent antioxidant properties, with recent studies highlighting its anti-inflammatory effect across various cell types. This study aims to explore the therapeutic potential of ORV in treating alopecia. We evaluated the effects of ORV on Human Follicle Dermal Papilla Cells (HFDPCs) and an androgenetic alopecia (AGA) mouse model. Oxidative stress in HFDPCs was induced using hydrogen peroxide (H2O2), and dihydrotestosterone (DHT) was used to simulate AGA in both HFDPCs and C57BL/6NJcl mice. Our finding demonstrated that ORV significantly enhanced HFDPCs proliferation. In H2O2-induced oxidative stress conditions, pretreatment with ORV decreased reactive oxygen species (ROS) levels and reduced the production of pro-inflammatory cytokine. In the AGA model, ORV inhibited β-Catenin phosphorylation in HFDPCs, thereby promoting hair growth and maintaining skin thickness, hair bulb size, and count in mice. Overall, ORV demonstrated anti-inflammatory and hair-regenerative effects in both in vitro and in vivo models of alopecia. These findings suggest that ORV is a promising candidate for the treatment of hair loss.
format Article
id doaj-art-a2aab9c8a5d047c5b5e0d6afabb3e66a
institution OA Journals
issn 2045-2322
language English
publishDate 2025-05-01
publisher Nature Portfolio
record_format Article
series Scientific Reports
spelling doaj-art-a2aab9c8a5d047c5b5e0d6afabb3e66a2025-08-20T01:53:12ZengNature PortfolioScientific Reports2045-23222025-05-0115111310.1038/s41598-025-02581-zOxyresveratrol enhances hair regeneration in human dermal papilla cell and androgenetic alopecia mouse modelHung Gia Tran0Aussavashai Shuayprom1Alisa Ruchusatsawat2Kornvalee Meesilpavikkai3Virgil A. S. H. Dalm4Kriangsak Ruchusatsawat5Jongkonnee Wongpiyabovorn6Center of Excellence in Immune-Mediated Diseases, Division of Immunology, Department of Microbiology, Faculty of Medicine, Chulalongkorn UniversityRegional Medical Sciences Center 3 Nakhonsawan, Department of Medical Sciences, Ministry of Public HealthEngineering Science Classroom, King Mongkut’s University of TechnologyDepartment of Microbiology, Faculty of Medicine, Chulalongkorn UniversityDepartment of Immunology, Laboratory Medical Immunology, Erasmus University Medical CenterMedical Sciences Technical Office, Department of Medical Sciences, Ministry of Public HealthCenter of Excellence in Immune-Mediated Diseases, Division of Immunology, Department of Microbiology, Faculty of Medicine, Chulalongkorn UniversityAbstract Alopecia, or hair loss, is a common dermatological condition caused by multiple factors. Oxyresveratrol (ORV), a compound derived from the heartwood of Artocarpus lakoocha, is recognized for its potent antioxidant properties, with recent studies highlighting its anti-inflammatory effect across various cell types. This study aims to explore the therapeutic potential of ORV in treating alopecia. We evaluated the effects of ORV on Human Follicle Dermal Papilla Cells (HFDPCs) and an androgenetic alopecia (AGA) mouse model. Oxidative stress in HFDPCs was induced using hydrogen peroxide (H2O2), and dihydrotestosterone (DHT) was used to simulate AGA in both HFDPCs and C57BL/6NJcl mice. Our finding demonstrated that ORV significantly enhanced HFDPCs proliferation. In H2O2-induced oxidative stress conditions, pretreatment with ORV decreased reactive oxygen species (ROS) levels and reduced the production of pro-inflammatory cytokine. In the AGA model, ORV inhibited β-Catenin phosphorylation in HFDPCs, thereby promoting hair growth and maintaining skin thickness, hair bulb size, and count in mice. Overall, ORV demonstrated anti-inflammatory and hair-regenerative effects in both in vitro and in vivo models of alopecia. These findings suggest that ORV is a promising candidate for the treatment of hair loss.https://doi.org/10.1038/s41598-025-02581-zAlopeciaAnti-inflammationHair regenerationDihydrotestosteroneOxyresveratrol
spellingShingle Hung Gia Tran
Aussavashai Shuayprom
Alisa Ruchusatsawat
Kornvalee Meesilpavikkai
Virgil A. S. H. Dalm
Kriangsak Ruchusatsawat
Jongkonnee Wongpiyabovorn
Oxyresveratrol enhances hair regeneration in human dermal papilla cell and androgenetic alopecia mouse model
Scientific Reports
Alopecia
Anti-inflammation
Hair regeneration
Dihydrotestosterone
Oxyresveratrol
title Oxyresveratrol enhances hair regeneration in human dermal papilla cell and androgenetic alopecia mouse model
title_full Oxyresveratrol enhances hair regeneration in human dermal papilla cell and androgenetic alopecia mouse model
title_fullStr Oxyresveratrol enhances hair regeneration in human dermal papilla cell and androgenetic alopecia mouse model
title_full_unstemmed Oxyresveratrol enhances hair regeneration in human dermal papilla cell and androgenetic alopecia mouse model
title_short Oxyresveratrol enhances hair regeneration in human dermal papilla cell and androgenetic alopecia mouse model
title_sort oxyresveratrol enhances hair regeneration in human dermal papilla cell and androgenetic alopecia mouse model
topic Alopecia
Anti-inflammation
Hair regeneration
Dihydrotestosterone
Oxyresveratrol
url https://doi.org/10.1038/s41598-025-02581-z
work_keys_str_mv AT hunggiatran oxyresveratrolenhanceshairregenerationinhumandermalpapillacellandandrogeneticalopeciamousemodel
AT aussavashaishuayprom oxyresveratrolenhanceshairregenerationinhumandermalpapillacellandandrogeneticalopeciamousemodel
AT alisaruchusatsawat oxyresveratrolenhanceshairregenerationinhumandermalpapillacellandandrogeneticalopeciamousemodel
AT kornvaleemeesilpavikkai oxyresveratrolenhanceshairregenerationinhumandermalpapillacellandandrogeneticalopeciamousemodel
AT virgilashdalm oxyresveratrolenhanceshairregenerationinhumandermalpapillacellandandrogeneticalopeciamousemodel
AT kriangsakruchusatsawat oxyresveratrolenhanceshairregenerationinhumandermalpapillacellandandrogeneticalopeciamousemodel
AT jongkonneewongpiyabovorn oxyresveratrolenhanceshairregenerationinhumandermalpapillacellandandrogeneticalopeciamousemodel