Precise copper ion release and recovery in polycaprolactone nanofiber scaffold: an antibacterial and osteogenic synergistic strategy for guided bone regeneration
IntroductionInfection control and bone regeneration remain major challenges in orthopedic therapy. To address these issues, we developed a multifunctional guided bone regeneration (GBR) nanofibrous material based on electrospun polycaprolactone (PCL). This material combines antibacterial and osteoge...
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Frontiers Media S.A.
2025-08-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2025.1650537/full |
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| author | Tongbin Liu Tongbin Liu Akram Hassan Matheel Zohair Yousif Alrawas Caiyun Cui Zaihan Ariffin |
| author_facet | Tongbin Liu Tongbin Liu Akram Hassan Matheel Zohair Yousif Alrawas Caiyun Cui Zaihan Ariffin |
| author_sort | Tongbin Liu |
| collection | DOAJ |
| description | IntroductionInfection control and bone regeneration remain major challenges in orthopedic therapy. To address these issues, we developed a multifunctional guided bone regeneration (GBR) nanofibrous material based on electrospun polycaprolactone (PCL). This material combines antibacterial and osteogenic properties using polydopamine (PDA) and copper ions (CuCu2+).MethodsPCL nanofibers were produced via electrospinning, and Cu2+ ions were introduced through PDA-mediated surface modification to enable pH-responsive binding and controlled release. The material’s physicochemical properties were evaluated through structural analysis, mechanical testing, and release kinetics. Biological performance was tested using antibacterial assays and osteoblast (MC3T3-E1) cell cultures, including assessments of cell proliferation and key osteogenic gene expression (Runx2, Osx, ALP, OCN).ResultsThe PCL-PDA-Cu composite showed strong structural integrity and mechanical stability. At a Cu2+ concentration of 0.1 M, it demonstrated: 1) strong antibacterial activity; 2) improved osteoblast proliferation; and 3) increased expression of osteogenic genes. The pH-dependent release system maintained effective Cu2+ levels while reducing cytotoxicity.DiscussionBy integrating PDA-mediated Cu2+ coordination with PCL nanofibers, we created a multifunctional platform that balances antimicrobial defense and bone regeneration. This controlled ion delivery strategy shows great promise for bone tissue engineering, especially in infection-prone environments. |
| format | Article |
| id | doaj-art-a2aa4000ff374ee69918ddfb01e755ae |
| institution | Kabale University |
| issn | 2296-634X |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj-art-a2aa4000ff374ee69918ddfb01e755ae2025-08-20T04:00:48ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2025-08-011310.3389/fcell.2025.16505371650537Precise copper ion release and recovery in polycaprolactone nanofiber scaffold: an antibacterial and osteogenic synergistic strategy for guided bone regenerationTongbin Liu0Tongbin Liu1Akram Hassan2Matheel Zohair Yousif Alrawas3Caiyun Cui4Zaihan Ariffin5School of Dental Sciences, Universiti Sains Malaysia Health Campus, Kubang Kerian, MalaysiaSchool of Stomatology, Qilu Medical University, Zibo City, Shandong, ChinaSchool of Dental Sciences, Universiti Sains Malaysia Health Campus, Kubang Kerian, MalaysiaSchool of Dental Sciences, Universiti Sains Malaysia Health Campus, Kubang Kerian, MalaysiaDepartment of Stomatology, Binzhou Medical University Hospital, Binzhou, Shandong, ChinaSchool of Dental Sciences, Universiti Sains Malaysia Health Campus, Kubang Kerian, MalaysiaIntroductionInfection control and bone regeneration remain major challenges in orthopedic therapy. To address these issues, we developed a multifunctional guided bone regeneration (GBR) nanofibrous material based on electrospun polycaprolactone (PCL). This material combines antibacterial and osteogenic properties using polydopamine (PDA) and copper ions (CuCu2+).MethodsPCL nanofibers were produced via electrospinning, and Cu2+ ions were introduced through PDA-mediated surface modification to enable pH-responsive binding and controlled release. The material’s physicochemical properties were evaluated through structural analysis, mechanical testing, and release kinetics. Biological performance was tested using antibacterial assays and osteoblast (MC3T3-E1) cell cultures, including assessments of cell proliferation and key osteogenic gene expression (Runx2, Osx, ALP, OCN).ResultsThe PCL-PDA-Cu composite showed strong structural integrity and mechanical stability. At a Cu2+ concentration of 0.1 M, it demonstrated: 1) strong antibacterial activity; 2) improved osteoblast proliferation; and 3) increased expression of osteogenic genes. The pH-dependent release system maintained effective Cu2+ levels while reducing cytotoxicity.DiscussionBy integrating PDA-mediated Cu2+ coordination with PCL nanofibers, we created a multifunctional platform that balances antimicrobial defense and bone regeneration. This controlled ion delivery strategy shows great promise for bone tissue engineering, especially in infection-prone environments.https://www.frontiersin.org/articles/10.3389/fcell.2025.1650537/fullelectrospinningpolycaprolactonepolydopaminecopper ionsantibacterial propertiesosteogenesis |
| spellingShingle | Tongbin Liu Tongbin Liu Akram Hassan Matheel Zohair Yousif Alrawas Caiyun Cui Zaihan Ariffin Precise copper ion release and recovery in polycaprolactone nanofiber scaffold: an antibacterial and osteogenic synergistic strategy for guided bone regeneration Frontiers in Cell and Developmental Biology electrospinning polycaprolactone polydopamine copper ions antibacterial properties osteogenesis |
| title | Precise copper ion release and recovery in polycaprolactone nanofiber scaffold: an antibacterial and osteogenic synergistic strategy for guided bone regeneration |
| title_full | Precise copper ion release and recovery in polycaprolactone nanofiber scaffold: an antibacterial and osteogenic synergistic strategy for guided bone regeneration |
| title_fullStr | Precise copper ion release and recovery in polycaprolactone nanofiber scaffold: an antibacterial and osteogenic synergistic strategy for guided bone regeneration |
| title_full_unstemmed | Precise copper ion release and recovery in polycaprolactone nanofiber scaffold: an antibacterial and osteogenic synergistic strategy for guided bone regeneration |
| title_short | Precise copper ion release and recovery in polycaprolactone nanofiber scaffold: an antibacterial and osteogenic synergistic strategy for guided bone regeneration |
| title_sort | precise copper ion release and recovery in polycaprolactone nanofiber scaffold an antibacterial and osteogenic synergistic strategy for guided bone regeneration |
| topic | electrospinning polycaprolactone polydopamine copper ions antibacterial properties osteogenesis |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2025.1650537/full |
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