Screening the Resistance of Male <i>Aedes aegypti</i> and <i>Anopheles coluzzii</i> to Insecticides in the Context of Using Genetic Control Tools in Burkina Faso

Background: Genetic control tools, such as the sterile insect technique (SIT) and genetically modified mosquitoes (GMMs), require releasing males comparable to their wild counterparts. Ensuring that released males do not exhibit higher insecticide resistance is critical. This study assessed the phen...

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Main Authors: Hamidou Maiga, Abel Souro Millogo, Koama Bayili, Etienne Bilgo, Inoussa Toe, Roch Kounbobr Dabiré, Jeremy Bouyer, Abdoulaye Diabaté
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Insects
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Online Access:https://www.mdpi.com/2075-4450/16/3/315
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author Hamidou Maiga
Abel Souro Millogo
Koama Bayili
Etienne Bilgo
Inoussa Toe
Roch Kounbobr Dabiré
Jeremy Bouyer
Abdoulaye Diabaté
author_facet Hamidou Maiga
Abel Souro Millogo
Koama Bayili
Etienne Bilgo
Inoussa Toe
Roch Kounbobr Dabiré
Jeremy Bouyer
Abdoulaye Diabaté
author_sort Hamidou Maiga
collection DOAJ
description Background: Genetic control tools, such as the sterile insect technique (SIT) and genetically modified mosquitoes (GMMs), require releasing males comparable to their wild counterparts. Ensuring that released males do not exhibit higher insecticide resistance is critical. This study assessed the phenotypic characteristics and insecticide susceptibility of key dengue and malaria vector species. Methods: Phenotypic resistance to deltamethrin (0.05%) was tested in two-to-five-day-old male and female <i>Aedes aegypti</i> (Linnaeus, 1762) (Borabora and Bobo strains) and <i>Anopheles coluzzii</i> (Coetzee & Wilkerson, 2013) (Vallee du Kou strain) using WHO susceptibility guidelines. Wing measurements of live and dead mosquitoes were used to assess body size. Results: Mortality rates were similar between male and female <i>Ae. aegypti</i> (Bobo strain) and <i>An. coluzzii</i>, while <i>Ae. aegypti</i> Borabora was fully susceptible in both sexes. Females were consistently larger than males, with significantly larger live females than dead ones in the <i>Ae. aegypti</i> Bobo strain. Conclusion: This study highlights sex-specific differences in body size and insecticide susceptibility. Integrating these analyses into vector management programs is essential for the success and sustainability of SIT- and GMM-based interventions targeting malaria and dengue vectors. Implications for integrating genetic control strategies are discussed.
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spelling doaj-art-a2a80f1abf9d48c594455dd9bb7b7fad2025-08-20T01:48:41ZengMDPI AGInsects2075-44502025-03-0116331510.3390/insects16030315Screening the Resistance of Male <i>Aedes aegypti</i> and <i>Anopheles coluzzii</i> to Insecticides in the Context of Using Genetic Control Tools in Burkina FasoHamidou Maiga0Abel Souro Millogo1Koama Bayili2Etienne Bilgo3Inoussa Toe4Roch Kounbobr Dabiré5Jeremy Bouyer6Abdoulaye Diabaté7Institut de Recherche en Sciences de la Santé, Bobo-Dioulasso 01 BP 545, Burkina FasoInstitut de Recherche en Sciences de la Santé, Bobo-Dioulasso 01 BP 545, Burkina FasoInstitut de Recherche en Sciences de la Santé, Bobo-Dioulasso 01 BP 545, Burkina FasoInstitut de Recherche en Sciences de la Santé, Bobo-Dioulasso 01 BP 545, Burkina FasoInstitut de Recherche en Sciences de la Santé, Bobo-Dioulasso 01 BP 545, Burkina FasoInstitut de Recherche en Sciences de la Santé, Bobo-Dioulasso 01 BP 545, Burkina FasoASTRE, Cirad-Département BIOS, INRAE, Université de Montpellier, Plateforme Technologique CYROI, Sainte-31 Clotilde, 97490 La Réunion, FranceInstitut de Recherche en Sciences de la Santé, Bobo-Dioulasso 01 BP 545, Burkina FasoBackground: Genetic control tools, such as the sterile insect technique (SIT) and genetically modified mosquitoes (GMMs), require releasing males comparable to their wild counterparts. Ensuring that released males do not exhibit higher insecticide resistance is critical. This study assessed the phenotypic characteristics and insecticide susceptibility of key dengue and malaria vector species. Methods: Phenotypic resistance to deltamethrin (0.05%) was tested in two-to-five-day-old male and female <i>Aedes aegypti</i> (Linnaeus, 1762) (Borabora and Bobo strains) and <i>Anopheles coluzzii</i> (Coetzee & Wilkerson, 2013) (Vallee du Kou strain) using WHO susceptibility guidelines. Wing measurements of live and dead mosquitoes were used to assess body size. Results: Mortality rates were similar between male and female <i>Ae. aegypti</i> (Bobo strain) and <i>An. coluzzii</i>, while <i>Ae. aegypti</i> Borabora was fully susceptible in both sexes. Females were consistently larger than males, with significantly larger live females than dead ones in the <i>Ae. aegypti</i> Bobo strain. Conclusion: This study highlights sex-specific differences in body size and insecticide susceptibility. Integrating these analyses into vector management programs is essential for the success and sustainability of SIT- and GMM-based interventions targeting malaria and dengue vectors. Implications for integrating genetic control strategies are discussed.https://www.mdpi.com/2075-4450/16/3/315mosquito controlsterile insect techniquemalesdeltamethrin
spellingShingle Hamidou Maiga
Abel Souro Millogo
Koama Bayili
Etienne Bilgo
Inoussa Toe
Roch Kounbobr Dabiré
Jeremy Bouyer
Abdoulaye Diabaté
Screening the Resistance of Male <i>Aedes aegypti</i> and <i>Anopheles coluzzii</i> to Insecticides in the Context of Using Genetic Control Tools in Burkina Faso
Insects
mosquito control
sterile insect technique
males
deltamethrin
title Screening the Resistance of Male <i>Aedes aegypti</i> and <i>Anopheles coluzzii</i> to Insecticides in the Context of Using Genetic Control Tools in Burkina Faso
title_full Screening the Resistance of Male <i>Aedes aegypti</i> and <i>Anopheles coluzzii</i> to Insecticides in the Context of Using Genetic Control Tools in Burkina Faso
title_fullStr Screening the Resistance of Male <i>Aedes aegypti</i> and <i>Anopheles coluzzii</i> to Insecticides in the Context of Using Genetic Control Tools in Burkina Faso
title_full_unstemmed Screening the Resistance of Male <i>Aedes aegypti</i> and <i>Anopheles coluzzii</i> to Insecticides in the Context of Using Genetic Control Tools in Burkina Faso
title_short Screening the Resistance of Male <i>Aedes aegypti</i> and <i>Anopheles coluzzii</i> to Insecticides in the Context of Using Genetic Control Tools in Burkina Faso
title_sort screening the resistance of male i aedes aegypti i and i anopheles coluzzii i to insecticides in the context of using genetic control tools in burkina faso
topic mosquito control
sterile insect technique
males
deltamethrin
url https://www.mdpi.com/2075-4450/16/3/315
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