Determination of Methomyl Residue in Tobacco Samples by Heart-Cutting Two-Dimensional Liquid Chromatography with Tandem Mass Spectrometry
A novel heart-cutting two-dimensional liquid chromatography coupled with tandem mass spectrometry (2D-LC-MS/MS) was developed for the qualitative and quantitative analysis of methomyl residue in tobacco. Compared to traditional methodologies, fairly high sensitivity and stability were achieved, and...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2020/8813142 |
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| author | Shihao Shen Min Chen Tiannan Wang Ting Fei Dianhai Yang Miaoling Cao Da Wu |
| author_facet | Shihao Shen Min Chen Tiannan Wang Ting Fei Dianhai Yang Miaoling Cao Da Wu |
| author_sort | Shihao Shen |
| collection | DOAJ |
| description | A novel heart-cutting two-dimensional liquid chromatography coupled with tandem mass spectrometry (2D-LC-MS/MS) was developed for the qualitative and quantitative analysis of methomyl residue in tobacco. Compared to traditional methodologies, fairly high sensitivity and stability were achieved, and the sample procedure was simplified in the two-dimensional liquid chromatography (2D-LC) method. Although methomyl had poor retention performance in most of the reversed-phase liquid chromatography (RPLC) columns, an effective RP/RP strategy was successfully facilitated. An XB-Phenyl column was employed in the first dimension to effectively remove thousands of interference compounds in the matrix. In the second dimension, an ADME column was applied for further separation. After optimization of the separation conditions, a six-way valve was utilized for direct transformation of the target fraction from the 1st column to the 2nd column. A dynamic range of 2.5 ng/mL to 500 ng/mL was achieved with correlation coefficient (r2) greater than 0.9995. The limit of detection and limit of quantification were determined to be 0.69 and 2.30 ng/mL, respectively. The 2D-LC method shows high sensitivity, good reproducibility, and recovery for methomyl in tobacco samples. Therefore, the new method was quite suitable for routine analysis. |
| format | Article |
| id | doaj-art-a2a61e28a7614f2da58dbd9042962739 |
| institution | OA Journals |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-a2a61e28a7614f2da58dbd90429627392025-08-20T02:20:37ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792020-01-01202010.1155/2020/88131428813142Determination of Methomyl Residue in Tobacco Samples by Heart-Cutting Two-Dimensional Liquid Chromatography with Tandem Mass SpectrometryShihao Shen0Min Chen1Tiannan Wang2Ting Fei3Dianhai Yang4Miaoling Cao5Da Wu6College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, ChinaTechnology R&D Center, Shanghai Tobacco Group Co., Ltd., 717 Changyang Road, Shanghai 200082, ChinaTechnology R&D Center, Shanghai Tobacco Group Co., Ltd., 717 Changyang Road, Shanghai 200082, ChinaTechnology R&D Center, Shanghai Tobacco Group Co., Ltd., 717 Changyang Road, Shanghai 200082, ChinaCollege of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, ChinaTechnology R&D Center, Shanghai Tobacco Group Co., Ltd., 717 Changyang Road, Shanghai 200082, ChinaTechnology R&D Center, Shanghai Tobacco Group Co., Ltd., 717 Changyang Road, Shanghai 200082, ChinaA novel heart-cutting two-dimensional liquid chromatography coupled with tandem mass spectrometry (2D-LC-MS/MS) was developed for the qualitative and quantitative analysis of methomyl residue in tobacco. Compared to traditional methodologies, fairly high sensitivity and stability were achieved, and the sample procedure was simplified in the two-dimensional liquid chromatography (2D-LC) method. Although methomyl had poor retention performance in most of the reversed-phase liquid chromatography (RPLC) columns, an effective RP/RP strategy was successfully facilitated. An XB-Phenyl column was employed in the first dimension to effectively remove thousands of interference compounds in the matrix. In the second dimension, an ADME column was applied for further separation. After optimization of the separation conditions, a six-way valve was utilized for direct transformation of the target fraction from the 1st column to the 2nd column. A dynamic range of 2.5 ng/mL to 500 ng/mL was achieved with correlation coefficient (r2) greater than 0.9995. The limit of detection and limit of quantification were determined to be 0.69 and 2.30 ng/mL, respectively. The 2D-LC method shows high sensitivity, good reproducibility, and recovery for methomyl in tobacco samples. Therefore, the new method was quite suitable for routine analysis.http://dx.doi.org/10.1155/2020/8813142 |
| spellingShingle | Shihao Shen Min Chen Tiannan Wang Ting Fei Dianhai Yang Miaoling Cao Da Wu Determination of Methomyl Residue in Tobacco Samples by Heart-Cutting Two-Dimensional Liquid Chromatography with Tandem Mass Spectrometry International Journal of Analytical Chemistry |
| title | Determination of Methomyl Residue in Tobacco Samples by Heart-Cutting Two-Dimensional Liquid Chromatography with Tandem Mass Spectrometry |
| title_full | Determination of Methomyl Residue in Tobacco Samples by Heart-Cutting Two-Dimensional Liquid Chromatography with Tandem Mass Spectrometry |
| title_fullStr | Determination of Methomyl Residue in Tobacco Samples by Heart-Cutting Two-Dimensional Liquid Chromatography with Tandem Mass Spectrometry |
| title_full_unstemmed | Determination of Methomyl Residue in Tobacco Samples by Heart-Cutting Two-Dimensional Liquid Chromatography with Tandem Mass Spectrometry |
| title_short | Determination of Methomyl Residue in Tobacco Samples by Heart-Cutting Two-Dimensional Liquid Chromatography with Tandem Mass Spectrometry |
| title_sort | determination of methomyl residue in tobacco samples by heart cutting two dimensional liquid chromatography with tandem mass spectrometry |
| url | http://dx.doi.org/10.1155/2020/8813142 |
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