Glissandra oviformis n. sp.: a novel predatory flagellate illuminates the character evolution within the eukaryotic clade CRuMs

Culturing protists offers a powerful approach to exploring eukaryotic diversity, especially for deep-branching lineages. In this study, we cultured and described a novel protist species, named Glissandra oviformis n sp. within the poorly studied and unclassified genus Glissandra. While an SSU rDNA g...

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Main Authors: Euki Yazaki, Ryo Harada, Ryu Isogai, Kohei Bamba, Ken-ichiro Ishida, Yuji Inagaki, Takashi Shiratori
Format: Article
Language:English
Published: The Royal Society 2025-06-01
Series:Open Biology
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Online Access:https://royalsocietypublishing.org/doi/10.1098/rsob.250057
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author Euki Yazaki
Ryo Harada
Ryu Isogai
Kohei Bamba
Ken-ichiro Ishida
Yuji Inagaki
Takashi Shiratori
author_facet Euki Yazaki
Ryo Harada
Ryu Isogai
Kohei Bamba
Ken-ichiro Ishida
Yuji Inagaki
Takashi Shiratori
author_sort Euki Yazaki
collection DOAJ
description Culturing protists offers a powerful approach to exploring eukaryotic diversity, especially for deep-branching lineages. In this study, we cultured and described a novel protist species, named Glissandra oviformis n sp. within the poorly studied and unclassified genus Glissandra. While an SSU rDNA gene phylogeny failed to resolve its phylogenetic placement in the eukaryotic tree, a phylogenomic analysis of 340 proteins indicated G. oviformis as a member of the CRuMs clade. Prior to this study, this clade consisted of diverse heterotrophic amoeba and flagellates, and lacked clear synapomorphies. Ultrastructural observations revealed that G. oviformis shares the characteristics with some CRuMs members, including the pellicle underlying the plasma membrane and an internal sleeve surrounding the central pair of the axoneme at the flagellar transitional region. Our findings suggest potential shared characteristics and synapomorphies for CRuMs and contribute to a deeper understanding of the character evolution within this clade.
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institution OA Journals
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publishDate 2025-06-01
publisher The Royal Society
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series Open Biology
spelling doaj-art-a2a3bf93524c435aa18cc5eca344396c2025-08-20T02:32:22ZengThe Royal SocietyOpen Biology2046-24412025-06-0115610.1098/rsob.250057Glissandra oviformis n. sp.: a novel predatory flagellate illuminates the character evolution within the eukaryotic clade CRuMsEuki Yazaki0Ryo Harada1Ryu Isogai2Kohei Bamba3Ken-ichiro Ishida4Yuji Inagaki5Takashi Shiratori6National Agriculture and Food Research Organization Advanced Analysis Center, Tsukuba, Ibaraki Prefecture, JapanDepartment of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, CanadaGraduate School of Science and Technology, University of Tsukuba, Tsukuba, Ibaraki Prefecture, JapanGraduate School of Science and Technology, University of Tsukuba, Tsukuba, Ibaraki Prefecture, JapanInstitute of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki Prefecture, JapanCenter for Computational Sciences, University of Tsukuba, Tsukuba, Ibaraki Prefecture, JapanInstitute of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki Prefecture, JapanCulturing protists offers a powerful approach to exploring eukaryotic diversity, especially for deep-branching lineages. In this study, we cultured and described a novel protist species, named Glissandra oviformis n sp. within the poorly studied and unclassified genus Glissandra. While an SSU rDNA gene phylogeny failed to resolve its phylogenetic placement in the eukaryotic tree, a phylogenomic analysis of 340 proteins indicated G. oviformis as a member of the CRuMs clade. Prior to this study, this clade consisted of diverse heterotrophic amoeba and flagellates, and lacked clear synapomorphies. Ultrastructural observations revealed that G. oviformis shares the characteristics with some CRuMs members, including the pellicle underlying the plasma membrane and an internal sleeve surrounding the central pair of the axoneme at the flagellar transitional region. Our findings suggest potential shared characteristics and synapomorphies for CRuMs and contribute to a deeper understanding of the character evolution within this clade.https://royalsocietypublishing.org/doi/10.1098/rsob.250057CRuMsprotists with uncertain phylogenetic affiliationsphylogenomicsultrastructures
spellingShingle Euki Yazaki
Ryo Harada
Ryu Isogai
Kohei Bamba
Ken-ichiro Ishida
Yuji Inagaki
Takashi Shiratori
Glissandra oviformis n. sp.: a novel predatory flagellate illuminates the character evolution within the eukaryotic clade CRuMs
Open Biology
CRuMs
protists with uncertain phylogenetic affiliations
phylogenomics
ultrastructures
title Glissandra oviformis n. sp.: a novel predatory flagellate illuminates the character evolution within the eukaryotic clade CRuMs
title_full Glissandra oviformis n. sp.: a novel predatory flagellate illuminates the character evolution within the eukaryotic clade CRuMs
title_fullStr Glissandra oviformis n. sp.: a novel predatory flagellate illuminates the character evolution within the eukaryotic clade CRuMs
title_full_unstemmed Glissandra oviformis n. sp.: a novel predatory flagellate illuminates the character evolution within the eukaryotic clade CRuMs
title_short Glissandra oviformis n. sp.: a novel predatory flagellate illuminates the character evolution within the eukaryotic clade CRuMs
title_sort glissandra oviformis n sp a novel predatory flagellate illuminates the character evolution within the eukaryotic clade crums
topic CRuMs
protists with uncertain phylogenetic affiliations
phylogenomics
ultrastructures
url https://royalsocietypublishing.org/doi/10.1098/rsob.250057
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