Human iPSC-Derived Neuron and Oligodendrocyte Co-culture as a Small-Molecule Screening Assay for Myelination
Neurons and oligodendrocytes are the building blocks of the brain. Neurons form synaptic connections and transmit signals, while oligodendrocytes, including oligodendrocyte precursor cells (OPCs) and their derivatives, are vital for central nervous system maintenance and myelination. The demand for...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bio-protocol LLC
2025-05-01
|
| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=5227&type=0 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849722394098270208 |
|---|---|
| author | Stefanie Chie Zsofia Szentpetery Melanie Generali Tanja Kuhlmann Giancarlo Natalucci Maria Miletta |
| author_facet | Stefanie Chie Zsofia Szentpetery Melanie Generali Tanja Kuhlmann Giancarlo Natalucci Maria Miletta |
| author_sort | Stefanie Chie |
| collection | DOAJ |
| description | Neurons and oligodendrocytes are the building blocks of the brain. Neurons form synaptic connections and transmit signals, while oligodendrocytes, including oligodendrocyte precursor cells (OPCs) and their derivatives, are vital for central nervous system maintenance and myelination. The demand for human-specific neuron-oligodendrocyte model systems to study these interactions has grown, yet co-culture protocols remain limited. Recent advancements in the field provide methods for deriving co-cultures of neurons and OPCs from human induced pluripotent stem cells (hiPSC), each with distinct benefits and challenges. This study presents a time-efficient, reproducible method to derive neurons and O4-expressing oligodendrocytes, followed by a straightforward co-culture system that minimizes astrocyte differentiation and ensures robust neuron and oligodendrocyte populations. |
| format | Article |
| id | doaj-art-a29bdce49d01416587b56cb641da0629 |
| institution | DOAJ |
| issn | 2331-8325 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Bio-protocol LLC |
| record_format | Article |
| series | Bio-Protocol |
| spelling | doaj-art-a29bdce49d01416587b56cb641da06292025-08-20T03:11:21ZengBio-protocol LLCBio-Protocol2331-83252025-05-0115910.21769/BioProtoc.5227Human iPSC-Derived Neuron and Oligodendrocyte Co-culture as a Small-Molecule Screening Assay for MyelinationStefanie Chie0Zsofia Szentpetery1Melanie Generali2Tanja Kuhlmann3Giancarlo Natalucci4Maria Miletta5Larsson-Rosenquist Foundation Center for Neurodevelopment, Growth and Nutrition of the Newborn, Department of Neonatology, University of Zurich and University Hospital Zurich, Zurich, SwitzerlandZNZ PhD program, University of Zurich, Zurich, SwitzerlandLarsson-Rosenquist Foundation Center for Neurodevelopment, Growth and Nutrition of the Newborn, Department of Neonatology, University of Zurich and University Hospital Zurich, Zurich, SwitzerlandInstitute for Regenerative Medicine (IREM), University of Zurich, Zurich, SwitzerlandInstitute of Neuropathology, University Hospital Münster, Münster, GermanyLarsson-Rosenquist Foundation Center for Neurodevelopment, Growth and Nutrition of the Newborn, Department of Neonatology, University of Zurich and University Hospital Zurich, Zurich, SwitzerlandNewborn Research Zurich, Department of Neonatology, University Hospital Zurich, Zurich, SwitzerlandLarsson-Rosenquist Foundation Center for Neurodevelopment, Growth and Nutrition of the Newborn, Department of Neonatology, University of Zurich and University Hospital Zurich, Zurich, SwitzerlandNeurons and oligodendrocytes are the building blocks of the brain. Neurons form synaptic connections and transmit signals, while oligodendrocytes, including oligodendrocyte precursor cells (OPCs) and their derivatives, are vital for central nervous system maintenance and myelination. The demand for human-specific neuron-oligodendrocyte model systems to study these interactions has grown, yet co-culture protocols remain limited. Recent advancements in the field provide methods for deriving co-cultures of neurons and OPCs from human induced pluripotent stem cells (hiPSC), each with distinct benefits and challenges. This study presents a time-efficient, reproducible method to derive neurons and O4-expressing oligodendrocytes, followed by a straightforward co-culture system that minimizes astrocyte differentiation and ensures robust neuron and oligodendrocyte populations.https://bio-protocol.org/en/bpdetail?id=5227&type=0 |
| spellingShingle | Stefanie Chie Zsofia Szentpetery Melanie Generali Tanja Kuhlmann Giancarlo Natalucci Maria Miletta Human iPSC-Derived Neuron and Oligodendrocyte Co-culture as a Small-Molecule Screening Assay for Myelination Bio-Protocol |
| title | Human iPSC-Derived Neuron and Oligodendrocyte Co-culture as a Small-Molecule Screening Assay for Myelination |
| title_full | Human iPSC-Derived Neuron and Oligodendrocyte Co-culture as a Small-Molecule Screening Assay for Myelination |
| title_fullStr | Human iPSC-Derived Neuron and Oligodendrocyte Co-culture as a Small-Molecule Screening Assay for Myelination |
| title_full_unstemmed | Human iPSC-Derived Neuron and Oligodendrocyte Co-culture as a Small-Molecule Screening Assay for Myelination |
| title_short | Human iPSC-Derived Neuron and Oligodendrocyte Co-culture as a Small-Molecule Screening Assay for Myelination |
| title_sort | human ipsc derived neuron and oligodendrocyte co culture as a small molecule screening assay for myelination |
| url | https://bio-protocol.org/en/bpdetail?id=5227&type=0 |
| work_keys_str_mv | AT stefaniechie humanipscderivedneuronandoligodendrocytecocultureasasmallmoleculescreeningassayformyelination AT zsofiaszentpetery humanipscderivedneuronandoligodendrocytecocultureasasmallmoleculescreeningassayformyelination AT melaniegenerali humanipscderivedneuronandoligodendrocytecocultureasasmallmoleculescreeningassayformyelination AT tanjakuhlmann humanipscderivedneuronandoligodendrocytecocultureasasmallmoleculescreeningassayformyelination AT giancarlonatalucci humanipscderivedneuronandoligodendrocytecocultureasasmallmoleculescreeningassayformyelination AT mariamiletta humanipscderivedneuronandoligodendrocytecocultureasasmallmoleculescreeningassayformyelination |