Effects of exosomes from human dental pulp stem cells on the biological behavior of human fibroblasts
Abstract The aim of this study was to investigate the effect of dental pulp stem cell-derived exosomes (DPSCs-Exos) on the biological behaviour of fibroblasts, particularly on keloid fibroblasts (KFs) and normal skin fibroblasts (NFs), with a view to providing new insights into cellular regenerative...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-78388-1 |
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| author | Guan-Yu Chen Ling-ling Fu Hui-ping Ye Ping Cheng Hong-chao Feng Ming Yan |
| author_facet | Guan-Yu Chen Ling-ling Fu Hui-ping Ye Ping Cheng Hong-chao Feng Ming Yan |
| author_sort | Guan-Yu Chen |
| collection | DOAJ |
| description | Abstract The aim of this study was to investigate the effect of dental pulp stem cell-derived exosomes (DPSCs-Exos) on the biological behaviour of fibroblasts, particularly on keloid fibroblasts (KFs) and normal skin fibroblasts (NFs), with a view to providing new insights into cellular regenerative medicine. We obtained DPSCs-Exos by ultracentrifugation and co-cultured it with KFs and NFs. We detected its effect on cell proliferation using the CCK-8 assay; cell migration ability by cell scratch and Transwell assays; extracellular matrix synthesis using the hydroxyproline content assay; the expression levels of genes associated with fibrosis by PCR assay; and the expression levels of proteins related to fibrosis in the cells using the Western Blot method. DPSCs-Exos was able to be taken up by fibroblasts after addition to the culture medium and affected the biological behavior of NFs and KFs. DPSCs-Exos promoted the proliferation of NFs, inhibited the migration and extracellular matrix synthesis of KFs. In addition, DPSCs-Exos was able to inhibit the expression of fibrosis-related genes and proteins in KFs. This study highlights the role of DPSCs-Exos in regulating the biological behaviour of fibroblasts, providing new insights for future applications in the field of cell-free regenerative medicine. |
| format | Article |
| id | doaj-art-a290f292b45049208aa07e7fe7cf06af |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-a290f292b45049208aa07e7fe7cf06af2025-01-12T12:23:22ZengNature PortfolioScientific Reports2045-23222025-01-0115111710.1038/s41598-024-78388-1Effects of exosomes from human dental pulp stem cells on the biological behavior of human fibroblastsGuan-Yu Chen0Ling-ling Fu1Hui-ping Ye2Ping Cheng3Hong-chao Feng4Ming Yan5College of Stomatology, Guizhou Medical UniversityDepartment of Oral Implantology, Guiyang Hospital of StomatologyDepartment of Otolaryngology, Guizhou Provincial People’s HospitalDepartment of Clinical Laboratory, Guiyang Hospital of StomatologyCollege of Stomatology, Guizhou Medical UniversityDepartment of Oral and Maxillofacial Surgery, Guiyang Hospital of StomatologyAbstract The aim of this study was to investigate the effect of dental pulp stem cell-derived exosomes (DPSCs-Exos) on the biological behaviour of fibroblasts, particularly on keloid fibroblasts (KFs) and normal skin fibroblasts (NFs), with a view to providing new insights into cellular regenerative medicine. We obtained DPSCs-Exos by ultracentrifugation and co-cultured it with KFs and NFs. We detected its effect on cell proliferation using the CCK-8 assay; cell migration ability by cell scratch and Transwell assays; extracellular matrix synthesis using the hydroxyproline content assay; the expression levels of genes associated with fibrosis by PCR assay; and the expression levels of proteins related to fibrosis in the cells using the Western Blot method. DPSCs-Exos was able to be taken up by fibroblasts after addition to the culture medium and affected the biological behavior of NFs and KFs. DPSCs-Exos promoted the proliferation of NFs, inhibited the migration and extracellular matrix synthesis of KFs. In addition, DPSCs-Exos was able to inhibit the expression of fibrosis-related genes and proteins in KFs. This study highlights the role of DPSCs-Exos in regulating the biological behaviour of fibroblasts, providing new insights for future applications in the field of cell-free regenerative medicine.https://doi.org/10.1038/s41598-024-78388-1Dental pulp stem cellsExosomesFibroblastsFibrosisBiological behaviour |
| spellingShingle | Guan-Yu Chen Ling-ling Fu Hui-ping Ye Ping Cheng Hong-chao Feng Ming Yan Effects of exosomes from human dental pulp stem cells on the biological behavior of human fibroblasts Scientific Reports Dental pulp stem cells Exosomes Fibroblasts Fibrosis Biological behaviour |
| title | Effects of exosomes from human dental pulp stem cells on the biological behavior of human fibroblasts |
| title_full | Effects of exosomes from human dental pulp stem cells on the biological behavior of human fibroblasts |
| title_fullStr | Effects of exosomes from human dental pulp stem cells on the biological behavior of human fibroblasts |
| title_full_unstemmed | Effects of exosomes from human dental pulp stem cells on the biological behavior of human fibroblasts |
| title_short | Effects of exosomes from human dental pulp stem cells on the biological behavior of human fibroblasts |
| title_sort | effects of exosomes from human dental pulp stem cells on the biological behavior of human fibroblasts |
| topic | Dental pulp stem cells Exosomes Fibroblasts Fibrosis Biological behaviour |
| url | https://doi.org/10.1038/s41598-024-78388-1 |
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