Evaluation of Lymphocyte Response to the Induced Oxidative Stress in a Cohort of Ageing Subjects, including Semisupercentenarians and Their Offspring
The production of reactive oxygen species (ROS) may promote immunosenescence if not counterbalanced by the antioxidant systems. Cell membranes, proteins, and nucleic acids become the target of ROS and progressively lose their structure and functions. This process could lead to an impairment of the i...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2018/7109312 |
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| author | Federico Sizzano Sebastiano Collino Ornella Cominetti Daniela Monti Paolo Garagnani Rita Ostan Chiara Pirazzini Maria Giulia Bacalini Daniela Mari Giuseppe Passarino Claudio Franceschi Alessio Palini |
| author_facet | Federico Sizzano Sebastiano Collino Ornella Cominetti Daniela Monti Paolo Garagnani Rita Ostan Chiara Pirazzini Maria Giulia Bacalini Daniela Mari Giuseppe Passarino Claudio Franceschi Alessio Palini |
| author_sort | Federico Sizzano |
| collection | DOAJ |
| description | The production of reactive oxygen species (ROS) may promote immunosenescence if not counterbalanced by the antioxidant systems. Cell membranes, proteins, and nucleic acids become the target of ROS and progressively lose their structure and functions. This process could lead to an impairment of the immune response. However, little is known about the capability of the immune cells of elderly individuals to dynamically counteract the oxidative stress. Here, the response of the main lymphocyte subsets to the induced oxidative stress in semisupercentenarians (CENT), their offspring (OFF), elderly controls (CTRL), and young individuals (YO) was analyzed using flow cytometry. The results showed that the ratio of the ROS levels between the induced and noninduced (I/NI) oxidative stress conditions was higher in CTRL and OFF than in CENT and YO, in almost all T, B, and NK subsets. Moreover, the ratio of reduced glutathione levels between I/NI conditions was higher in OFF and CENT compared to the other groups in almost all the subsets. Finally, we observed significant correlations between the response to the induced oxidative stress and the degree of methylation in specific genes on the oxidative stress pathway. Globally, these data suggest that the capability to buffer dynamic changes in the oxidative environment could be a hallmark of longevity in humans. |
| format | Article |
| id | doaj-art-a28eb1f906eb4fb695a9a86e56ffb9e8 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-a28eb1f906eb4fb695a9a86e56ffb9e82025-02-03T01:28:06ZengWileyMediators of Inflammation0962-93511466-18612018-01-01201810.1155/2018/71093127109312Evaluation of Lymphocyte Response to the Induced Oxidative Stress in a Cohort of Ageing Subjects, including Semisupercentenarians and Their OffspringFederico Sizzano0Sebastiano Collino1Ornella Cominetti2Daniela Monti3Paolo Garagnani4Rita Ostan5Chiara Pirazzini6Maria Giulia Bacalini7Daniela Mari8Giuseppe Passarino9Claudio Franceschi10Alessio Palini11Flow Cytometry, Nestlè Institute of Health Sciences, EPFL Campus, 1010 Lausanne, SwitzerlandMolecular Biomarkers, Nestlè Institute of Health Sciences, EPFL Campus, 1010 Lausanne, SwitzerlandMolecular Biomarkers, Nestlè Institute of Health Sciences, EPFL Campus, 1010 Lausanne, SwitzerlandDepartment of Experimental and Clinical Biomedical Sciences, University of Florence, Viale Morgagni 50, 50134 Florence, ItalyDepartment of Experimental, Diagnostic and Specialty Medicine, Experimental Pathology, University of Bologna, Via S. Giacomo 12, 40126 Bologna, ItalyInterdepartmental Centre “L. Galvani” (CIG), University of Bologna, Via S. Giacomo 12, 40126 Bologna, ItalyIRCCS, Institute of Neurological Sciences of Bologna, Ospedale Bellaria, Via Altura 3, 40139 Bologna, ItalyIRCCS, Institute of Neurological Sciences of Bologna, Ospedale Bellaria, Via Altura 3, 40139 Bologna, ItalyGeriatric Unit, Department of Medical Sciences and Community Health, University of Milan, Via Pace 9, 20122 Milan, ItalyDepartment of Ecology, Biology, and Earth Sciences, University of Calabria, Rende, ItalyDepartment of Experimental, Diagnostic and Specialty Medicine, Experimental Pathology, University of Bologna, Via S. Giacomo 12, 40126 Bologna, ItalyFlow Cytometry, Nestlè Institute of Health Sciences, EPFL Campus, 1010 Lausanne, SwitzerlandThe production of reactive oxygen species (ROS) may promote immunosenescence if not counterbalanced by the antioxidant systems. Cell membranes, proteins, and nucleic acids become the target of ROS and progressively lose their structure and functions. This process could lead to an impairment of the immune response. However, little is known about the capability of the immune cells of elderly individuals to dynamically counteract the oxidative stress. Here, the response of the main lymphocyte subsets to the induced oxidative stress in semisupercentenarians (CENT), their offspring (OFF), elderly controls (CTRL), and young individuals (YO) was analyzed using flow cytometry. The results showed that the ratio of the ROS levels between the induced and noninduced (I/NI) oxidative stress conditions was higher in CTRL and OFF than in CENT and YO, in almost all T, B, and NK subsets. Moreover, the ratio of reduced glutathione levels between I/NI conditions was higher in OFF and CENT compared to the other groups in almost all the subsets. Finally, we observed significant correlations between the response to the induced oxidative stress and the degree of methylation in specific genes on the oxidative stress pathway. Globally, these data suggest that the capability to buffer dynamic changes in the oxidative environment could be a hallmark of longevity in humans.http://dx.doi.org/10.1155/2018/7109312 |
| spellingShingle | Federico Sizzano Sebastiano Collino Ornella Cominetti Daniela Monti Paolo Garagnani Rita Ostan Chiara Pirazzini Maria Giulia Bacalini Daniela Mari Giuseppe Passarino Claudio Franceschi Alessio Palini Evaluation of Lymphocyte Response to the Induced Oxidative Stress in a Cohort of Ageing Subjects, including Semisupercentenarians and Their Offspring Mediators of Inflammation |
| title | Evaluation of Lymphocyte Response to the Induced Oxidative Stress in a Cohort of Ageing Subjects, including Semisupercentenarians and Their Offspring |
| title_full | Evaluation of Lymphocyte Response to the Induced Oxidative Stress in a Cohort of Ageing Subjects, including Semisupercentenarians and Their Offspring |
| title_fullStr | Evaluation of Lymphocyte Response to the Induced Oxidative Stress in a Cohort of Ageing Subjects, including Semisupercentenarians and Their Offspring |
| title_full_unstemmed | Evaluation of Lymphocyte Response to the Induced Oxidative Stress in a Cohort of Ageing Subjects, including Semisupercentenarians and Their Offspring |
| title_short | Evaluation of Lymphocyte Response to the Induced Oxidative Stress in a Cohort of Ageing Subjects, including Semisupercentenarians and Their Offspring |
| title_sort | evaluation of lymphocyte response to the induced oxidative stress in a cohort of ageing subjects including semisupercentenarians and their offspring |
| url | http://dx.doi.org/10.1155/2018/7109312 |
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