Human Myeloma Cell Lines Induce Osteoblast Downregulation of CD99 Which Is Involved in Osteoblast Formation and Activity
CD99 is a transmembrane glycoprotein expressed in physiological conditions by cells of different tissues, including osteoblasts (OBs). High or low CD99 levels have been detected in various pathological conditions, and the supernatant of some carcinoma cell lines can modulate CD99 expression in OB-li...
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| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/156787 |
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| _version_ | 1850171155959250944 |
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| author | Angela Oranger Giacomina Brunetti Claudia Carbone Graziana Colaianni Teresa Mongelli Isabella Gigante Roberto Tamma Giorgio Mori Adriana Di Benedetto Marika Sciandra Selena Ventura Katia Scotlandi Silvia Colucci Maria Grano |
| author_facet | Angela Oranger Giacomina Brunetti Claudia Carbone Graziana Colaianni Teresa Mongelli Isabella Gigante Roberto Tamma Giorgio Mori Adriana Di Benedetto Marika Sciandra Selena Ventura Katia Scotlandi Silvia Colucci Maria Grano |
| author_sort | Angela Oranger |
| collection | DOAJ |
| description | CD99 is a transmembrane glycoprotein expressed in physiological conditions by cells of different tissues, including osteoblasts (OBs). High or low CD99 levels have been detected in various pathological conditions, and the supernatant of some carcinoma cell lines can modulate CD99 expression in OB-like cells. In the present work we demonstrate for the first time that two different human myeloma cell lines (H929 and U266) and, in a less degree, their conditioned media significantly downregulate CD99 expression in normal human OBs during the differentiation process. In the same experimental conditions the OBs display a less differentiated phenotype as demonstrated by the decreased expression of RUNX2 and Collagen I. On the contrary, when CD99 was activated by using a specific agonist antibody, the OBs become more active as demonstrated by the upregulation of Alkaline Phosphatase, Collagen I, RUNX2, and JUND expression. Furthermore, we demonstrate that the activation of CD99 is able to induce the phosphorylation of ERK 1/2 and AKT intracellular signal transduction molecules in the OBs. |
| format | Article |
| id | doaj-art-a28b0de44f7145b7a0c8ed4981eb47c6 |
| institution | OA Journals |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-a28b0de44f7145b7a0c8ed4981eb47c62025-08-20T02:20:19ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/156787156787Human Myeloma Cell Lines Induce Osteoblast Downregulation of CD99 Which Is Involved in Osteoblast Formation and ActivityAngela Oranger0Giacomina Brunetti1Claudia Carbone2Graziana Colaianni3Teresa Mongelli4Isabella Gigante5Roberto Tamma6Giorgio Mori7Adriana Di Benedetto8Marika Sciandra9Selena Ventura10Katia Scotlandi11Silvia Colucci12Maria Grano13Department of Basic Medical Sciences, Neurosciences and Sense Organs, Section of Human Anatomy and Histology, University of Bari, Piazza Giulio Cesare 11, 70124 Bari, ItalyDepartment of Basic Medical Sciences, Neurosciences and Sense Organs, Section of Human Anatomy and Histology, University of Bari, Piazza Giulio Cesare 11, 70124 Bari, ItalyDepartment of Basic Medical Sciences, Neurosciences and Sense Organs, Section of Human Anatomy and Histology, University of Bari, Piazza Giulio Cesare 11, 70124 Bari, ItalyDepartment of Basic Medical Sciences, Neurosciences and Sense Organs, Section of Human Anatomy and Histology, University of Bari, Piazza Giulio Cesare 11, 70124 Bari, ItalyDepartment of Basic Medical Sciences, Neurosciences and Sense Organs, Section of Human Anatomy and Histology, University of Bari, Piazza Giulio Cesare 11, 70124 Bari, ItalyDepartment of Basic Medical Sciences, Neurosciences and Sense Organs, Section of Human Anatomy and Histology, University of Bari, Piazza Giulio Cesare 11, 70124 Bari, ItalyDepartment of Basic Medical Sciences, Neurosciences and Sense Organs, Section of Human Anatomy and Histology, University of Bari, Piazza Giulio Cesare 11, 70124 Bari, ItalyDepartment of Clinical and Experimental Medicine, Medical School, University of Foggia, Viale Luigi Pinto 1, 71100 Foggia, ItalyDepartment of Basic Medical Sciences, Neurosciences and Sense Organs, Section of Human Anatomy and Histology, University of Bari, Piazza Giulio Cesare 11, 70124 Bari, ItalyCRS Development of Biomolecular Therapies, Laboratory of Experimental Oncology, Orthopaedic Institute of Rizzoli, Via di Barbiano 1/10, 40136 Bologna, ItalyCRS Development of Biomolecular Therapies, Laboratory of Experimental Oncology, Orthopaedic Institute of Rizzoli, Via di Barbiano 1/10, 40136 Bologna, ItalyCRS Development of Biomolecular Therapies, Laboratory of Experimental Oncology, Orthopaedic Institute of Rizzoli, Via di Barbiano 1/10, 40136 Bologna, ItalyDepartment of Basic Medical Sciences, Neurosciences and Sense Organs, Section of Human Anatomy and Histology, University of Bari, Piazza Giulio Cesare 11, 70124 Bari, ItalyDepartment of Basic Medical Sciences, Neurosciences and Sense Organs, Section of Human Anatomy and Histology, University of Bari, Piazza Giulio Cesare 11, 70124 Bari, ItalyCD99 is a transmembrane glycoprotein expressed in physiological conditions by cells of different tissues, including osteoblasts (OBs). High or low CD99 levels have been detected in various pathological conditions, and the supernatant of some carcinoma cell lines can modulate CD99 expression in OB-like cells. In the present work we demonstrate for the first time that two different human myeloma cell lines (H929 and U266) and, in a less degree, their conditioned media significantly downregulate CD99 expression in normal human OBs during the differentiation process. In the same experimental conditions the OBs display a less differentiated phenotype as demonstrated by the decreased expression of RUNX2 and Collagen I. On the contrary, when CD99 was activated by using a specific agonist antibody, the OBs become more active as demonstrated by the upregulation of Alkaline Phosphatase, Collagen I, RUNX2, and JUND expression. Furthermore, we demonstrate that the activation of CD99 is able to induce the phosphorylation of ERK 1/2 and AKT intracellular signal transduction molecules in the OBs.http://dx.doi.org/10.1155/2015/156787 |
| spellingShingle | Angela Oranger Giacomina Brunetti Claudia Carbone Graziana Colaianni Teresa Mongelli Isabella Gigante Roberto Tamma Giorgio Mori Adriana Di Benedetto Marika Sciandra Selena Ventura Katia Scotlandi Silvia Colucci Maria Grano Human Myeloma Cell Lines Induce Osteoblast Downregulation of CD99 Which Is Involved in Osteoblast Formation and Activity Journal of Immunology Research |
| title | Human Myeloma Cell Lines Induce Osteoblast Downregulation of CD99 Which Is Involved in Osteoblast Formation and Activity |
| title_full | Human Myeloma Cell Lines Induce Osteoblast Downregulation of CD99 Which Is Involved in Osteoblast Formation and Activity |
| title_fullStr | Human Myeloma Cell Lines Induce Osteoblast Downregulation of CD99 Which Is Involved in Osteoblast Formation and Activity |
| title_full_unstemmed | Human Myeloma Cell Lines Induce Osteoblast Downregulation of CD99 Which Is Involved in Osteoblast Formation and Activity |
| title_short | Human Myeloma Cell Lines Induce Osteoblast Downregulation of CD99 Which Is Involved in Osteoblast Formation and Activity |
| title_sort | human myeloma cell lines induce osteoblast downregulation of cd99 which is involved in osteoblast formation and activity |
| url | http://dx.doi.org/10.1155/2015/156787 |
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