Occlusal hypofunction affects the gene expression of osteoblast and primary cilia in the alveolar bone: An experimental study
[Objective:] To investigate whether the gene expression changes of the primary cilia and osteoblast in the alveolar bone are associated with occlusal hypofunction. [Methods:] The unloading model was established in the right mandible on 6-week-old wild type (WT) male mice and the left side was used a...
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Editorial Office of Journal of Oral and Maxillofacial Surgery
2023-08-01
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| Series: | Kouqiang hemian waike zazhi |
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| Online Access: | https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2023.04.001 |
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| _version_ | 1849225118477189120 |
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| author | HU Lingwei SUN Yao |
| author_facet | HU Lingwei SUN Yao |
| author_sort | HU Lingwei |
| collection | DOAJ |
| description | [Objective:] To investigate whether the gene expression changes of the primary cilia and osteoblast in the alveolar bone are associated with occlusal hypofunction. [Methods:] The unloading model was established in the right mandible on 6-week-old wild type (WT) male mice and the left side was used as a self-control study. Samples were sacrificed 2 weeks after the operation, and the parameters of alveolar bone were scanned and analyzed by micro-CT. The changes in the number and position of osteoblasts and osteoclasts were observed histologically, and real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of osteoblastogenesis and osteoclastogenesis. The changes in the number and location of primary cilia were observed by co-staining, and the expression of cilium-related genes was detected. The Pearson correlation analysis was used to detect the expression of cilium-related genes and osteoblastogenesis, and the colocalization was observed by co-staining. [Results:] Analysis of the unloading side revealed a significant decrease in bone volume/total volume (BV/TV) (P<0.05). The number of osteoblasts decreased while the number of osteoclasts increased significantly (P<0.01). Both the occurrence rate of primary cilia and expression of cilium-related genes showed a decreasing trend (P<0.05). Furthermore, the expression of osteoblastogenesis and intraflagellar transport genes were significantly correlated (P<0.05). Co-staining results showed that osteoblasts were located on the top of root furcation, and there were primary cilia on the surface. [Conclusion:] The parameters of alveolar bone on the experimental side were significantly decreased and bone resorption accelerated on the unloading side. Both the number of osteoblasts and primary cilia as well as the expression of related genes decreased significantly and the expression levels of related genes were positively correlated. |
| format | Article |
| id | doaj-art-a285b3924f7f4efe887c260edb652f8a |
| institution | Kabale University |
| issn | 1005-4979 |
| language | zho |
| publishDate | 2023-08-01 |
| publisher | Editorial Office of Journal of Oral and Maxillofacial Surgery |
| record_format | Article |
| series | Kouqiang hemian waike zazhi |
| spelling | doaj-art-a285b3924f7f4efe887c260edb652f8a2025-08-25T06:10:14ZzhoEditorial Office of Journal of Oral and Maxillofacial SurgeryKouqiang hemian waike zazhi1005-49792023-08-0133420321010.12439/kqhm.1005-4979.2023.04.001Occlusal hypofunction affects the gene expression of osteoblast and primary cilia in the alveolar bone: An experimental studyHU Lingwei0SUN Yao1Department of Oral Implantology, Stomatological Hospital and Dental School of Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, ChinaDepartment of Oral Implantology, Stomatological Hospital and Dental School of Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, China[Objective:] To investigate whether the gene expression changes of the primary cilia and osteoblast in the alveolar bone are associated with occlusal hypofunction. [Methods:] The unloading model was established in the right mandible on 6-week-old wild type (WT) male mice and the left side was used as a self-control study. Samples were sacrificed 2 weeks after the operation, and the parameters of alveolar bone were scanned and analyzed by micro-CT. The changes in the number and position of osteoblasts and osteoclasts were observed histologically, and real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of osteoblastogenesis and osteoclastogenesis. The changes in the number and location of primary cilia were observed by co-staining, and the expression of cilium-related genes was detected. The Pearson correlation analysis was used to detect the expression of cilium-related genes and osteoblastogenesis, and the colocalization was observed by co-staining. [Results:] Analysis of the unloading side revealed a significant decrease in bone volume/total volume (BV/TV) (P<0.05). The number of osteoblasts decreased while the number of osteoclasts increased significantly (P<0.01). Both the occurrence rate of primary cilia and expression of cilium-related genes showed a decreasing trend (P<0.05). Furthermore, the expression of osteoblastogenesis and intraflagellar transport genes were significantly correlated (P<0.05). Co-staining results showed that osteoblasts were located on the top of root furcation, and there were primary cilia on the surface. [Conclusion:] The parameters of alveolar bone on the experimental side were significantly decreased and bone resorption accelerated on the unloading side. Both the number of osteoblasts and primary cilia as well as the expression of related genes decreased significantly and the expression levels of related genes were positively correlated.https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2023.04.001occlusal hypofunctionosteoblastprimary ciliaalveolar bone remolding |
| spellingShingle | HU Lingwei SUN Yao Occlusal hypofunction affects the gene expression of osteoblast and primary cilia in the alveolar bone: An experimental study Kouqiang hemian waike zazhi occlusal hypofunction osteoblast primary cilia alveolar bone remolding |
| title | Occlusal hypofunction affects the gene expression of osteoblast and primary cilia in the alveolar bone: An experimental study |
| title_full | Occlusal hypofunction affects the gene expression of osteoblast and primary cilia in the alveolar bone: An experimental study |
| title_fullStr | Occlusal hypofunction affects the gene expression of osteoblast and primary cilia in the alveolar bone: An experimental study |
| title_full_unstemmed | Occlusal hypofunction affects the gene expression of osteoblast and primary cilia in the alveolar bone: An experimental study |
| title_short | Occlusal hypofunction affects the gene expression of osteoblast and primary cilia in the alveolar bone: An experimental study |
| title_sort | occlusal hypofunction affects the gene expression of osteoblast and primary cilia in the alveolar bone an experimental study |
| topic | occlusal hypofunction osteoblast primary cilia alveolar bone remolding |
| url | https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2023.04.001 |
| work_keys_str_mv | AT hulingwei occlusalhypofunctionaffectsthegeneexpressionofosteoblastandprimaryciliainthealveolarboneanexperimentalstudy AT sunyao occlusalhypofunctionaffectsthegeneexpressionofosteoblastandprimaryciliainthealveolarboneanexperimentalstudy |