Biochar-based fertilizer increases soil nutrients and enhances tea quality: a metabolomics-based analysis

IntroductionBiochar-based fertilizers (BF) have emerged as a promising strategy to improve soil physicochemical and biological properties, thereby enhancing tea yield and quality.MethodsA field experiment was conducted using two types of BF- ordinary BF (BF1, containing 15% biochar) and optimized BF...

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Main Authors: Zhenyu Yang, Zetao Ren, Xiangzhen Zhu, Wenyan Yang, Zhengqian Ye, Luming Tian, Jiawei Ma
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-05-01
Series:Frontiers in Plant Science
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Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2025.1552759/full
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author Zhenyu Yang
Zetao Ren
Xiangzhen Zhu
Wenyan Yang
Zhengqian Ye
Luming Tian
Jiawei Ma
author_facet Zhenyu Yang
Zetao Ren
Xiangzhen Zhu
Wenyan Yang
Zhengqian Ye
Luming Tian
Jiawei Ma
author_sort Zhenyu Yang
collection DOAJ
description IntroductionBiochar-based fertilizers (BF) have emerged as a promising strategy to improve soil physicochemical and biological properties, thereby enhancing tea yield and quality.MethodsA field experiment was conducted using two types of BF- ordinary BF (BF1, containing 15% biochar) and optimized BF (BF2, containing 30% biochar)- applied for either 1 or 2 years. The effects on the soil nutrients, tea plant growth, tea quality, and metabolomics profiles were assessed.ResultsResults showed that BF application significantly increased soil pH and nutrient availability, as well as the bud length and hundred-bud weight (p < 0.05). Notably, BF2 applied for 2 years significantly increased the content of free amino acids, total flavonoids, soluble sugar, while reduced the phenol-ammonia ratio (p < 0.05), thereby improving tea quality. Further metabolomics analysis revealed that BF2 treatment significantly elevated the levels of amino acids, including theanine, threonine, proline, valine, and glutamic acid, while decreasing catechins including C, EC, and EGCG, thus leading to reduced bitterness and astringency and enhanced freshness. Besides, differential metabolites were mainly involved in amino acid and flavonoid biosynthesis pathway.DiscussionTaken together, prolonged BF2 application significantly improved soil fertility, promoted tea growth, and enriched flavor-related metabolites, offering valuable insights for optimizing fertilization strategies in tea plantations.
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publisher Frontiers Media S.A.
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spelling doaj-art-a277bc7d85de40e3ac16f5c2fa823c232025-08-20T03:21:46ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-05-011610.3389/fpls.2025.15527591552759Biochar-based fertilizer increases soil nutrients and enhances tea quality: a metabolomics-based analysisZhenyu Yang0Zetao Ren1Xiangzhen Zhu2Wenyan Yang3Zhengqian Ye4Luming Tian5Jiawei Ma6College of Tea Science and Tea Culture, Zhejiang A & F University, Hangzhou, Zhejiang, ChinaCollege of Resources and Environment, Zhejiang A & F University, Hangzhou, Zhejiang, ChinaCollege of Tea Science and Tea Culture, Zhejiang A & F University, Hangzhou, Zhejiang, ChinaCollege of Tea Science and Tea Culture, Zhejiang A & F University, Hangzhou, Zhejiang, ChinaCollege of Resources and Environment, Zhejiang A & F University, Hangzhou, Zhejiang, ChinaSoil, Fertilizer, Plant Protection and Energy Center of Qingyuan Agriculture and rural Bureau, Lishui, Zhejiang, ChinaCollege of Tea Science and Tea Culture, Zhejiang A & F University, Hangzhou, Zhejiang, ChinaIntroductionBiochar-based fertilizers (BF) have emerged as a promising strategy to improve soil physicochemical and biological properties, thereby enhancing tea yield and quality.MethodsA field experiment was conducted using two types of BF- ordinary BF (BF1, containing 15% biochar) and optimized BF (BF2, containing 30% biochar)- applied for either 1 or 2 years. The effects on the soil nutrients, tea plant growth, tea quality, and metabolomics profiles were assessed.ResultsResults showed that BF application significantly increased soil pH and nutrient availability, as well as the bud length and hundred-bud weight (p < 0.05). Notably, BF2 applied for 2 years significantly increased the content of free amino acids, total flavonoids, soluble sugar, while reduced the phenol-ammonia ratio (p < 0.05), thereby improving tea quality. Further metabolomics analysis revealed that BF2 treatment significantly elevated the levels of amino acids, including theanine, threonine, proline, valine, and glutamic acid, while decreasing catechins including C, EC, and EGCG, thus leading to reduced bitterness and astringency and enhanced freshness. Besides, differential metabolites were mainly involved in amino acid and flavonoid biosynthesis pathway.DiscussionTaken together, prolonged BF2 application significantly improved soil fertility, promoted tea growth, and enriched flavor-related metabolites, offering valuable insights for optimizing fertilization strategies in tea plantations.https://www.frontiersin.org/articles/10.3389/fpls.2025.1552759/fullbiochar-based fertilizersoil nutrientstea plant growthtea qualitymetabolomics
spellingShingle Zhenyu Yang
Zetao Ren
Xiangzhen Zhu
Wenyan Yang
Zhengqian Ye
Luming Tian
Jiawei Ma
Biochar-based fertilizer increases soil nutrients and enhances tea quality: a metabolomics-based analysis
Frontiers in Plant Science
biochar-based fertilizer
soil nutrients
tea plant growth
tea quality
metabolomics
title Biochar-based fertilizer increases soil nutrients and enhances tea quality: a metabolomics-based analysis
title_full Biochar-based fertilizer increases soil nutrients and enhances tea quality: a metabolomics-based analysis
title_fullStr Biochar-based fertilizer increases soil nutrients and enhances tea quality: a metabolomics-based analysis
title_full_unstemmed Biochar-based fertilizer increases soil nutrients and enhances tea quality: a metabolomics-based analysis
title_short Biochar-based fertilizer increases soil nutrients and enhances tea quality: a metabolomics-based analysis
title_sort biochar based fertilizer increases soil nutrients and enhances tea quality a metabolomics based analysis
topic biochar-based fertilizer
soil nutrients
tea plant growth
tea quality
metabolomics
url https://www.frontiersin.org/articles/10.3389/fpls.2025.1552759/full
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